Open Access
Metabolites, volume 13, issue 11, pages 1128
Analysis of 4-Hydroxyphenyllactic Acid and Other Diagnostically Important Metabolites of α-Amino Acids in Human Blood Serum Using a Validated and Sensitive Ultra-High-Pressure Liquid Chromatography-Tandem Mass Spectrometry Method
Pavel D Sobolev
1
,
Natalia A. Burnakova
1
,
Natalia V. Beloborodova
2
,
Alexander I Revelsky
3
,
Alisa K. Pautova
2
1
Exacte Labs Bioanalytical Laboratory, 20-2 Nauchny Proezd, 117246 Moscow, Russia
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Publication type: Journal Article
Publication date: 2023-11-03
Journal:
Metabolites
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 4.1
ISSN: 22181989, 22181989
Biochemistry
Molecular Biology
Endocrinology, Diabetes and Metabolism
Abstract
The profile of and dynamic concentration changes in tyrosine, phenylalanine, and tryptophan metabolites in blood are of great interest since they could be considered potential biomarkers of different disorders. Some aromatic metabolites, such as 4-hydroxyphenyllactic, 4-hydroxyphenylacetic, phenyllactic, and 4-hydroxybenzoic acids have previously demonstrated their diagnostic significance in critically ill patients and patients with post-COVID-19 syndrome. In this study, a sensitive method, including serum protein precipitation with methanol and ultra-high-pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection, was developed and validated for six phenyl- and five indole-containing acids in human serum. The liquid–liquid extraction was also examined, but it demonstrated unsatisfactory results based on analyte recoveries and the matrix effect. However, the recoveries for all analytes reached 100% and matrix effects were not observed using protein precipitation. This made it possible to use deionized water as a blank matrix. The lower limits of quantitation (LLOQs) were from 0.02 to 0.25 μmol/L. The validated method was used for the analysis of serum samples of healthy volunteers (n = 48) to reveal the reference values of the target analytes. The concentrations of the most clinically significant metabolite 4-hydroxyphenyllactic acid, which were revealed using UPLC-MS/MS and a previously developed gas chromatography-mass spectrometry method, were completely comparable. The proposed UPLC-MS/MS protocol can be used in the routine clinical practice of medical centers.
Top-30
Citations by journals
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Russian Chemical Reviews
1 publication, 100%
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Citations by publishers
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 100%
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Sobolev P. D. et al. Analysis of 4-Hydroxyphenyllactic Acid and Other Diagnostically Important Metabolites of α-Amino Acids in Human Blood Serum Using a Validated and Sensitive Ultra-High-Pressure Liquid Chromatography-Tandem Mass Spectrometry Method // Metabolites. 2023. Vol. 13. No. 11. p. 1128.
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Sobolev P. D., Burnakova N. A., Beloborodova N. V., Revelsky A. I., Pautova A. K. Analysis of 4-Hydroxyphenyllactic Acid and Other Diagnostically Important Metabolites of α-Amino Acids in Human Blood Serum Using a Validated and Sensitive Ultra-High-Pressure Liquid Chromatography-Tandem Mass Spectrometry Method // Metabolites. 2023. Vol. 13. No. 11. p. 1128.
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TY - JOUR
DO - 10.3390/metabo13111128
UR - https://doi.org/10.3390/metabo13111128
TI - Analysis of 4-Hydroxyphenyllactic Acid and Other Diagnostically Important Metabolites of α-Amino Acids in Human Blood Serum Using a Validated and Sensitive Ultra-High-Pressure Liquid Chromatography-Tandem Mass Spectrometry Method
T2 - Metabolites
AU - Sobolev, Pavel D
AU - Burnakova, Natalia A.
AU - Beloborodova, Natalia V.
AU - Revelsky, Alexander I
AU - Pautova, Alisa K.
PY - 2023
DA - 2023/11/03 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1128
IS - 11
VL - 13
SN - 2218-1989
SN - 2218-1989
ER -
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@article{2023_Sobolev,
author = {Pavel D Sobolev and Natalia A. Burnakova and Natalia V. Beloborodova and Alexander I Revelsky and Alisa K. Pautova},
title = {Analysis of 4-Hydroxyphenyllactic Acid and Other Diagnostically Important Metabolites of α-Amino Acids in Human Blood Serum Using a Validated and Sensitive Ultra-High-Pressure Liquid Chromatography-Tandem Mass Spectrometry Method},
journal = {Metabolites},
year = {2023},
volume = {13},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390/metabo13111128},
number = {11},
pages = {1128},
doi = {10.3390/metabo13111128}
}
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MLA
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Sobolev, Pavel D., et al. “Analysis of 4-Hydroxyphenyllactic Acid and Other Diagnostically Important Metabolites of α-Amino Acids in Human Blood Serum Using a Validated and Sensitive Ultra-High-Pressure Liquid Chromatography-Tandem Mass Spectrometry Method.” Metabolites, vol. 13, no. 11, Nov. 2023, p. 1128. https://doi.org/10.3390/metabo13111128.