Open Access
Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips
Tatyana Pleshakova
1
,
Anna Kaysheva
1
,
Ivan Shumov
1
,
Vadim Ziborov
1, 2
,
Jana Bayzyanova
3
,
Vladimir Konev
3
,
Vasiliy Uchaikin
3
,
Alexander Archakov
1
,
Yuri A. Ivanov
1
Publication type: Journal Article
Publication date: 2019-02-15
PubMed ID:
30781415
Electrical and Electronic Engineering
Mechanical Engineering
Control and Systems Engineering
Abstract
In the present study, we demonstrate atomic force microscopy (AFM)-based detection of hepatitis C virus (HCV) particles in serum samples using a chip with aptamer-functionalized surface (apta-based AFM chip). The target particles, containing core antigen of HCV (HCVcoreAg protein), were biospecifically captured onto the chip surface from 1 mL of test solution containing 10 µL of serum collected from a hepatitis C patient. The registration of aptamer/antigen complexes on the chip surface was performed by AFM. The aptamers used in the present study were initially developed for therapeutic purposes; herein, these aptamers have been successfully utilized as probe molecules for HCVcoreAg detection in the presence of a complex protein matrix (human serum). The results obtained herein can be used for the development of detection systems that employ affine enrichment for protein detection.
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49
Total citations:
49
Citations from 2024:
10
(20.4%)
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GOST
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Pleshakova T. et al. Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips // Micromachines. 2019. Vol. 10. No. 2. p. 129.
GOST all authors (up to 50)
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Pleshakova T., Kaysheva A., Shumov I., Ziborov V., Bayzyanova J., Konev V., Uchaikin V., Archakov A., Ivanov Y. A. Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips // Micromachines. 2019. Vol. 10. No. 2. p. 129.
Cite this
RIS
Copy
TY - JOUR
DO - 10.3390/mi10020129
UR - https://doi.org/10.3390/mi10020129
TI - Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips
T2 - Micromachines
AU - Pleshakova, Tatyana
AU - Kaysheva, Anna
AU - Shumov, Ivan
AU - Ziborov, Vadim
AU - Bayzyanova, Jana
AU - Konev, Vladimir
AU - Uchaikin, Vasiliy
AU - Archakov, Alexander
AU - Ivanov, Yuri A.
PY - 2019
DA - 2019/02/15
PB - MDPI
SP - 129
IS - 2
VL - 10
PMID - 30781415
SN - 2072-666X
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2019_Pleshakova,
author = {Tatyana Pleshakova and Anna Kaysheva and Ivan Shumov and Vadim Ziborov and Jana Bayzyanova and Vladimir Konev and Vasiliy Uchaikin and Alexander Archakov and Yuri A. Ivanov},
title = {Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips},
journal = {Micromachines},
year = {2019},
volume = {10},
publisher = {MDPI},
month = {feb},
url = {https://doi.org/10.3390/mi10020129},
number = {2},
pages = {129},
doi = {10.3390/mi10020129}
}
Cite this
MLA
Copy
Pleshakova, Tatyana, et al. “Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips.” Micromachines, vol. 10, no. 2, Feb. 2019, p. 129. https://doi.org/10.3390/mi10020129.