Open Access
Nanomaterials, volume 10, issue 12, pages 2563
Plasmon-enhanced fluorescence of EGFP on short-range ordered ag nanohole arrays
Publication type: Journal Article
Publication date: 2020-12-20
PubMed ID:
33419362
General Chemical Engineering
General Materials Science
Abstract
Fluorescence of organic molecules can be enhanced by plasmonic nanostructures through coupling to their locally amplified electromagnetic field, resulting in higher brightness and better photostability of fluorophores, which is particularly important for bioimaging applications involving fluorescent proteins as genetically encoded biomarkers. Here, we show that a hybrid bionanosystem comprised of a monolayer of Enhanced Green Fluorescent Protein (EGFP) covalently linked to optically thin Ag films with short-range ordered nanohole arrays can exhibit up to 6-fold increased brightness. The largest enhancement factor is observed for nanohole arrays with a propagating surface plasmon mode, tuned to overlap with both excitation and emission of EGFP. The fluorescence lifetime measurements in combination with FDTD simulations provide in-depth insight into the origin of the fluorescence enhancement, showing that the effect is due to the local amplification of the optical field near the edges of the nanoholes. Our results pave the way to improving the photophysical properties of hybrid bionanosystems based on fluorescent proteins at the interface with easily fabricated and tunable plasmonic nanostructures.
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ACS applied materials & interfaces
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ACS applied materials & interfaces
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American Chemical Society (ACS)
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American Chemical Society (ACS)
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Bochenkov V. et al. Plasmon-enhanced fluorescence of EGFP on short-range ordered ag nanohole arrays // Nanomaterials. 2020. Vol. 10. No. 12. p. 2563.
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Bochenkov V., Lobanova E. M., SHAKHOV A. M., Astafiev A. A., Bogdanov A. M., Timoshenko V. A., Bochenkova A. V. Plasmon-enhanced fluorescence of EGFP on short-range ordered ag nanohole arrays // Nanomaterials. 2020. Vol. 10. No. 12. p. 2563.
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TY - JOUR
DO - 10.3390/nano10122563
UR - https://doi.org/10.3390%2Fnano10122563
TI - Plasmon-enhanced fluorescence of EGFP on short-range ordered ag nanohole arrays
T2 - Nanomaterials
AU - Lobanova, Ekaterina M
AU - Astafiev, Artyom A
AU - Bochenkov, V.E.
AU - SHAKHOV, A. M.
AU - Bogdanov, Alexey M
AU - Timoshenko, Vadim A
AU - Bochenkova, Anastasia V.
PY - 2020
DA - 2020/12/20 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 2563
IS - 12
VL - 10
PMID - 33419362
SN - 2079-4991
ER -
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@article{2020_Bochenkov,
author = {Ekaterina M Lobanova and Artyom A Astafiev and V.E. Bochenkov and A. M. SHAKHOV and Alexey M Bogdanov and Vadim A Timoshenko and Anastasia V. Bochenkova},
title = {Plasmon-enhanced fluorescence of EGFP on short-range ordered ag nanohole arrays},
journal = {Nanomaterials},
year = {2020},
volume = {10},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {dec},
url = {https://doi.org/10.3390%2Fnano10122563},
number = {12},
pages = {2563},
doi = {10.3390/nano10122563}
}
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Bochenkov, V.E., et al. “Plasmon-enhanced fluorescence of EGFP on short-range ordered ag nanohole arrays.” Nanomaterials, vol. 10, no. 12, Dec. 2020, p. 2563. https://doi.org/10.3390%2Fnano10122563.