Open Access

Nanomaterials

, volume 12 , issue 1 , pages 126

Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood

Pavel V Khramtsov ¹

Maria Bochkova ¹

V. P. Timganova ¹

Anton Nechaev ²

Sofya Uzhviyuk ¹

Kseniya Shardina ¹

Irina Maslennikova ¹

Mikhail Rayev ¹

S. A. Zamorina ¹

Hide authors affiliations Show authors affiliations: 2 affiliations

Institute of Ecology and Genetics of Microorganisms UB RAS, 13 Golev str., 614081 Perm, Russia |

Institute of Technical Chemistry UB RAS, 3 Academician Korolev str., 614013 Perm, Russia |

Publication type: Journal Article

Publication date: 2021-12-30

MDPI

Nanomaterials

scimago Q1

wos Q2

SJR: 0.811

CiteScore: 9.2

Impact factor: 4.3

ISSN: 20794991

DOI: 10.3390/nano12010126

Copy DOI

PubMed ID: 35010076

General Chemical Engineering

General Materials Science

Abstract

Multiple graphene-based therapeutics have recently been developed, however potential risks related to the interaction between nanomaterials and immune cells are still poorly understood. Therefore, studying the impact of graphene oxide on various populations of immune cells is of importance. In this work, we aimed to investigate the effects of PEGylated graphene oxide on monocytes isolated from human peripheral blood. Graphene oxide nanoparticles with lateral sizes of 100–200 nm and 1–5 μm were modified with linear and branched PEG (GO-PEG). Size, elemental composition, and structure of the resulting nanoparticles were characterized. We confirmed that PEG was successfully attached to the graphene oxide surface. The influence of GO-PEG on the production of reactive oxygen species (ROS), cytokines, phagocytosis, and viability of monocytes was studied. Uptake of GO-PEG by monocytes depends on PEG structure (linear or branched). Branched PEG decreased the number of GO-PEG nanoparticles per monocyte. The viability of monocytes was not altered by co-cultivation with GO-PEG. GO-PEG decreased the phagocytosis of Escherichia coli in a concentration-dependent manner. ROS formation by monocytes was determined by measuring luminol-, lucigenin-, and dichlorodihydrofluorescein-dependent luminescence. GO-PEG decreased luminescent signal probably due to inactivation of ROS, such as hydroxyl and superoxide radicals. Some types of GO-PEG stimulated secretion of IL-10 by monocytes, but this effect did not correlate with their size or PEG structure.

Found

4 citations

Khramtsov Pavel

🥼

DSc in Biological/biomedical sciences

83 publications, 408 citations, 7 reviews

h-index: 10

Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences

Perm State National Research University

2 citations

Vlasova Violetta

PhD in Biological/biomedical sciences

24 publications, 16 citations

h-index: 2

Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences

Perm State National Research University

Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences

1 citation

Saidakova Evgeniya

🤝

DSc in Biological/biomedical sciences, associate professor

60 publications, 293 citations

h-index: 7

Perm State National Research University

Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences

Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences

Research interests

Metabolism

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Khramtsov P. V. et al. Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood // Nanomaterials. 2021. Vol. 12. No. 1. p. 126.

GOST all authors (up to 50) Copy

Khramtsov P. V., Bochkova M., Timganova V. P., Nechaev A., Uzhviyuk S., Shardina K., Maslennikova I., Rayev M., Zamorina S. A. Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood // Nanomaterials. 2021. Vol. 12. No. 1. p. 126.

RIS |

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RIS Copy

TY - JOUR

DO - 10.3390/nano12010126

UR - https://www.mdpi.com/2079-4991/12/1/126

TI - Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood

T2 - Nanomaterials

AU - Khramtsov, Pavel V

AU - Bochkova, Maria

AU - Timganova, V. P.

AU - Nechaev, Anton

AU - Uzhviyuk, Sofya

AU - Shardina, Kseniya

AU - Maslennikova, Irina

AU - Rayev, Mikhail

AU - Zamorina, S. A.

PY - 2021

DA - 2021/12/30

PB - MDPI

SP - 126

IS - 1

VL - 12

PMID - 35010076

SN - 2079-4991

ER -

BibTex |

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BibTex (up to 50 authors) Copy

@article{2021_Khramtsov,

author = {Pavel V Khramtsov and Maria Bochkova and V. P. Timganova and Anton Nechaev and Sofya Uzhviyuk and Kseniya Shardina and Irina Maslennikova and Mikhail Rayev and S. A. Zamorina},

title = {Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood},

journal = {Nanomaterials},

year = {2021},

volume = {12},

publisher = {MDPI},

month = {dec},

url = {https://www.mdpi.com/2079-4991/12/1/126},

number = {1},

pages = {126},

doi = {10.3390/nano12010126}

}

MLA

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MLA Copy

Khramtsov, Pavel V., et al. “Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood.” Nanomaterials, vol. 12, no. 1, Dec. 2021, p. 126. https://www.mdpi.com/2079-4991/12/1/126.

Publisher

MDPI

Journal

Nanomaterials

scimago Q1

wos Q2

SJR

0.811

CiteScore

9.2

Impact factor

4.3

ISSN

20794991 (Electronic)

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