Open Access
Nanomaterials, volume 12, issue 21, pages 3854
Lithographic SERS Aptasensor for Ultrasensitive Detection of SARS-CoV-2 in Biological Fluids
Vladimir Kukushkin
1
,
Oganes Ambartsumyan
2
,
Anna Astrakhantseva
1, 3
,
В. А. Гущин
4
,
Alexandra Nikonova
5
,
Anastasia Dorofeeva
5
,
Vitaly Zverev
2, 5
,
Alexandra Gambaryan
6
,
Daria Tikhonova
1, 7
,
Timofei Sovetnikov
8
,
Assel Akhmetova
8
,
Igor Yaminsky
8
,
1
Publication type: Journal Article
Publication date: 2022-11-01
General Chemical Engineering
General Materials Science
Abstract
In this paper, we propose a technology for the rapid and sensitive detection of the whole viral particles of SARS-CoV-2 using double-labeled DNA aptamers as recognition elements together with the SERS method for detecting the optical response. We report on the development of a SERS-aptasensor based on a reproducible lithographic SERS substrate, featuring the combination of high speed, specificity, and ultrasensitive quantitative detection of SARS-CoV-2 virions. The sensor makes it possible to identify SARS-CoV-2 in very low concentrations (the limit of detection was 100 copies/mL), demonstrating a sensitivity level comparable to the existing diagnostic golden standard—the reverse transcription polymerase chain reaction.
Top-30
Citations by journals
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1
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Frontiers in Bioengineering and Biotechnology
1 publication, 20%
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International Journal of Molecular Sciences
1 publication, 20%
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Sensors and Actuators, B: Chemical
1 publication, 20%
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Russian Chemical Reviews
1 publication, 20%
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Advanced Optical Materials
1 publication, 20%
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1
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Citations by publishers
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1
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Frontiers Media S.A.
1 publication, 20%
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 20%
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Elsevier
1 publication, 20%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 20%
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Wiley
1 publication, 20%
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1
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Kukushkin V. et al. Lithographic SERS Aptasensor for Ultrasensitive Detection of SARS-CoV-2 in Biological Fluids // Nanomaterials. 2022. Vol. 12. No. 21. p. 3854.
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Kukushkin V., Ambartsumyan O., Astrakhantseva A., Гущин В. А., Nikonova A., Dorofeeva A., Zverev V., Gambaryan A., Tikhonova D., Sovetnikov T., Akhmetova A., Yaminsky I., Zavyalova E. Lithographic SERS Aptasensor for Ultrasensitive Detection of SARS-CoV-2 in Biological Fluids // Nanomaterials. 2022. Vol. 12. No. 21. p. 3854.
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TY - JOUR
DO - 10.3390/nano12213854
UR - https://doi.org/10.3390/nano12213854
TI - Lithographic SERS Aptasensor for Ultrasensitive Detection of SARS-CoV-2 in Biological Fluids
T2 - Nanomaterials
AU - Kukushkin, Vladimir
AU - Ambartsumyan, Oganes
AU - Astrakhantseva, Anna
AU - Гущин, В. А.
AU - Nikonova, Alexandra
AU - Dorofeeva, Anastasia
AU - Zverev, Vitaly
AU - Gambaryan, Alexandra
AU - Tikhonova, Daria
AU - Sovetnikov, Timofei
AU - Akhmetova, Assel
AU - Yaminsky, Igor
AU - Zavyalova, Elena
PY - 2022
DA - 2022/11/01 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 3854
IS - 21
VL - 12
SN - 2079-4991
ER -
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@article{2022_Kukushkin,
author = {Vladimir Kukushkin and Oganes Ambartsumyan and Anna Astrakhantseva and В. А. Гущин and Alexandra Nikonova and Anastasia Dorofeeva and Vitaly Zverev and Alexandra Gambaryan and Daria Tikhonova and Timofei Sovetnikov and Assel Akhmetova and Igor Yaminsky and Elena Zavyalova},
title = {Lithographic SERS Aptasensor for Ultrasensitive Detection of SARS-CoV-2 in Biological Fluids},
journal = {Nanomaterials},
year = {2022},
volume = {12},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390/nano12213854},
number = {21},
pages = {3854},
doi = {10.3390/nano12213854}
}
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MLA
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Kukushkin, Vladimir, et al. “Lithographic SERS Aptasensor for Ultrasensitive Detection of SARS-CoV-2 in Biological Fluids.” Nanomaterials, vol. 12, no. 21, Nov. 2022, p. 3854. https://doi.org/10.3390/nano12213854.