Open Access

Processes

, volume 10 , issue 2 , pages 383

Human Cytochrome P450 2C9 and Its Polymorphic Modifications: Electroanalysis, Catalytic Properties, and Approaches to the Regulation of Enzymatic Activity

Victoria V Shumyantseva ^{1, 2}

Tatiana V. Bulko ¹

Polina I Koroleva ¹

Evgeniya V Shikh ³

Anna A Makhova ³

Maryia S Kisel ⁴

Irina V Haidukevich ⁴

Andrei A. Gilep ^{1, 4}

Hide authors affiliations Show authors affiliations: 4 affiliations

Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Build 8, 119121 Moscow, Russia |

Faculty of Biomedicine, Pirogov Russian National Research Medical University, Ostrovitianov Street, 1, 117997 Moscow, Russia |

Department of Clinical Pharmacology and Propaedeutics of Internal Diseases, I.M. Sechenov First Moscow Medical State University (Sechenov University), Trubetskaya Str., 8/2, 119991 Moscow, Russia |

⁴

Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 220141 Minsk, Belarus |

Publication type: Journal Article

Publication date: 2022-02-17

MDPI

Processes

scimago Q2

wos Q3

SJR: 0.554

CiteScore: 5.5

Impact factor: 2.8

ISSN: 22279717

DOI: 10.3390/pr10020383

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Process Chemistry and Technology

Bioengineering

Chemical Engineering (miscellaneous)

Abstract

The electrochemical properties of cytochrome P450 2C9 (CYP2C9) and polymorphic modifications P450 2C9*2 (CYP2C9*2) and P450 2C9*3 (CYP2C9*3) were studied. To analyze the comparative electrochemical and electrocatalytic activity, the enzymes were immobilized on electrodes modified with a membrane-like synthetic surfactant (didodecyldimethylammonium bromide (DDAB)). An adequate choice of the type of modified electrode was confirmed by cyclic voltammetry of cytochromes P450 under anaerobic conditions, demonstrating well-defined peaks of reduction and oxidation of the heme iron. The midpoint potential, Emid, of cytochrome P450 2C9 is −0.318 ± 0.01 V, and Emid = −0.324 ± 0.01 V, and Emid = −0.318 ± 0.03 V for allelic variant 2C9*2 and allelic variant 2C9*3, respectively. In the presence of substrate diclofenac under aerobic conditions, cytochrome P450 2C9 and its polymorphic modifications P450 2C9*2 and P450 2C9*3 exhibit catalytic properties. Stimulation of the metabolism of diclofenac by cytochrome P450 2C9 in the presence of antioxidant medications mexidol and taurine was shown.

Found

1 citation

Zvereva Maria

DSc in Chemistry, professor

102 publications, 1 295 citations

h-index: 19

Lomonosov Moscow State University

1 citation

Le-Deygen Irina

🥼 🤝

PhD in Chemistry

68 publications, 2 744 citations, 8 reviews

h-index: 19

Lomonosov Moscow State University

Research interests

Antibiotics

Chitosan

Drug delivery systems

Polymers for drug delivery

Proteins

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Shumyantseva V. V. et al. Human Cytochrome P450 2C9 and Its Polymorphic Modifications: Electroanalysis, Catalytic Properties, and Approaches to the Regulation of Enzymatic Activity // Processes. 2022. Vol. 10. No. 2. p. 383.

GOST all authors (up to 50) Copy

Shumyantseva V. V., Bulko T. V., Koroleva P. I., Shikh E. V., Makhova A. A., Kisel M. S., Haidukevich I. V., Gilep A. A. Human Cytochrome P450 2C9 and Its Polymorphic Modifications: Electroanalysis, Catalytic Properties, and Approaches to the Regulation of Enzymatic Activity // Processes. 2022. Vol. 10. No. 2. p. 383.

RIS |

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RIS Copy

TY - JOUR

DO - 10.3390/pr10020383

UR - https://doi.org/10.3390/pr10020383

TI - Human Cytochrome P450 2C9 and Its Polymorphic Modifications: Electroanalysis, Catalytic Properties, and Approaches to the Regulation of Enzymatic Activity

T2 - Processes

AU - Shumyantseva, Victoria V

AU - Bulko, Tatiana V.

AU - Koroleva, Polina I

AU - Shikh, Evgeniya V

AU - Makhova, Anna A

AU - Kisel, Maryia S

AU - Haidukevich, Irina V

AU - Gilep, Andrei A.

PY - 2022

DA - 2022/02/17

PB - MDPI

SP - 383

IS - 2

VL - 10

SN - 2227-9717

ER -

BibTex |

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BibTex (up to 50 authors) Copy

@article{2022_Shumyantseva,

author = {Victoria V Shumyantseva and Tatiana V. Bulko and Polina I Koroleva and Evgeniya V Shikh and Anna A Makhova and Maryia S Kisel and Irina V Haidukevich and Andrei A. Gilep},

title = {Human Cytochrome P450 2C9 and Its Polymorphic Modifications: Electroanalysis, Catalytic Properties, and Approaches to the Regulation of Enzymatic Activity},

journal = {Processes},

year = {2022},

volume = {10},

publisher = {MDPI},

month = {feb},

url = {https://doi.org/10.3390/pr10020383},

number = {2},

pages = {383},

doi = {10.3390/pr10020383}

}

MLA

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MLA Copy

Shumyantseva, Victoria V., et al. “Human Cytochrome P450 2C9 and Its Polymorphic Modifications: Electroanalysis, Catalytic Properties, and Approaches to the Regulation of Enzymatic Activity.” Processes, vol. 10, no. 2, Feb. 2022, p. 383. https://doi.org/10.3390/pr10020383.

Publisher

MDPI

Journal

Processes

scimago Q2

wos Q3

SJR

0.554

CiteScore

5.5

Impact factor

2.8

ISSN

22279717 (Electronic)