Open Access

Voprosy Virusologii, volume 66, issue 6, pages 434-441

DNA mapping in the capsid of giant bacteriophage phiEL (Caudovirales: Myoviridae: Elvirus) by analytical electron microscopy

Trifonova T S

Sokolova Olga S.

Moiseenko A V

Bourkaltseva Maria

Shaburova O. V.

Shaytan Alexey K

Krylov V N

Publication type: Journal Article

Publication date: 2022-01-08

Central Research Institute for Epidemiology

Journal: Voprosy Virusologii

Quartile SCImago

Quartile WOS

—

Impact factor: —

ISSN: 05074088

DOI: 10.36233/0507-4088-80

Copy DOI

General Medicine

Infectious Diseases

Virology

Abstract

Introduction. Giant phiKZ-like bacteriophages have a unique protein formation inside the capsid, an inner body (IB) with supercoiled DNA molecule wrapped around it. Standard cryo-electron microscopy (cryo-EM) approaches do not allow to distinguish this structure from the surrounding nucleic acid of the phage. We previously developed an analytical approach to visualize protein-DNA complexes on Escherichia coli bacterial cell slices using the chemical element phosphorus as a marker. In the study presented, we adapted this technique for much smaller objects, namely the capsids of phiKZ-like bacteriophages.

Material and methods. Following electron microscopy techniques were used in the study: analytical (AEM) (electron energy loss spectroscopy, EELS), and cryo-EM (images of samples subjected to low and high dose of electron irradiation were compared).

Results. We studied DNA packaging inside the capsids of giant bacteriophages phiEL from the Myoviridae family that infect Pseudomonas aeruginosa. Phosphorus distribution maps were obtained, showing an asymmetrical arrangement of DNA inside the capsid.

Discussion. We developed and applied an IB imaging technique using a high angle dark-field detector (HAADF) and the STEM-EELS analytical approach. Phosphorus mapping by EELS and cryo-electron microscopy revealed a protein formation as IB within the phage phiEL capsid. The size of IB was estimated using theoretical calculations.

Conclusion. The developed technique can be applied to study the distribution of phosphorus in other DNA- or RNA-containing viruses at relatively low concentrations of the element sought.

By date By citations

Citations by journals

	1
Biomedicines	Biomedicines, 1, 100% Biomedicines 1 publication, 100%
	1

Citations by publishers

	1
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 100% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 100%
	1

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2022],"ids":[0],"codes":[0],"imageUrls":[""],"datasets":[{"label":"Citations number","data":[1],"backgroundColor":["#3B82F6"],"percentage":["100"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Biomedicines"],"ids":[24421],"codes":[0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp"],"datasets":[{"label":"","data":[1],"backgroundColor":["#3B82F6"],"percentage":[100],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)"],"ids":[202],"codes":[0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp"],"datasets":[{"label":"","data":[1],"backgroundColor":["#3B82F6"],"percentage":[100],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Metrics

Cite this

GOST |

Cite this

GOST Copy

Trifonova T. S. et al. DNA mapping in the capsid of giant bacteriophage phiEL (Caudovirales: Myoviridae: Elvirus) by analytical electron microscopy // Voprosy Virusologii. 2022. Vol. 66. No. 6. pp. 434-441.

GOST all authors (up to 50) Copy

Trifonova T. S., Moiseenko A. V., Bourkaltseva M., Shaburova O. V., Shaytan A. K., Krylov V. N., Sokolova O. S. DNA mapping in the capsid of giant bacteriophage phiEL (Caudovirales: Myoviridae: Elvirus) by analytical electron microscopy // Voprosy Virusologii. 2022. Vol. 66. No. 6. pp. 434-441.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.36233/0507-4088-80

UR - https://doi.org/10.36233%2F0507-4088-80

TI - DNA mapping in the capsid of giant bacteriophage phiEL (Caudovirales: Myoviridae: Elvirus) by analytical electron microscopy

T2 - Voprosy Virusologii

AU - Trifonova, T S

AU - Moiseenko, A V

AU - Bourkaltseva, Maria

AU - Shaburova, O. V.

AU - Shaytan, Alexey K

AU - Krylov, V N

AU - Sokolova, Olga S.

PY - 2022

DA - 2022/01/08 00:00:00

PB - Central Research Institute for Epidemiology

SP - 434-441

IS - 6

VL - 66

SN - 0507-4088

ER -

BibTex |

Cite this

BibTex Copy

@article{2022_Trifonova,

author = {T S Trifonova and A V Moiseenko and Maria Bourkaltseva and O. V. Shaburova and Alexey K Shaytan and V N Krylov and Olga S. Sokolova},

title = {DNA mapping in the capsid of giant bacteriophage phiEL (Caudovirales: Myoviridae: Elvirus) by analytical electron microscopy},

journal = {Voprosy Virusologii},

year = {2022},

volume = {66},

publisher = {Central Research Institute for Epidemiology},

month = {jan},

url = {https://doi.org/10.36233%2F0507-4088-80},

number = {6},

pages = {434--441},

doi = {10.36233/0507-4088-80}

}

MLA

Cite this

MLA Copy

Trifonova, T. S., et al. “DNA mapping in the capsid of giant bacteriophage phiEL (Caudovirales: Myoviridae: Elvirus) by analytical electron microscopy.” Voprosy Virusologii, vol. 66, no. 6, Jan. 2022, pp. 434-441. https://doi.org/10.36233%2F0507-4088-80.

Found error?

Publisher

Central Research Institute for Epidemiology

Journal

Voprosy Virusologii

Quartile SCImago

Quartile WOS

—

Impact factor

—

ISSN

05074088 (Print)

Profiles