volume 13 issue 3 pages 181-198

Flow cytometry: principles, applications and recent advances

Publication typeJournal Article
Publication date2021-02-05
scimago Q2
wos Q3
SJR0.437
CiteScore3.4
Impact factor1.8
ISSN17576180, 17576199
General Medicine
Clinical Biochemistry
Analytical Chemistry
General Pharmacology, Toxicology and Pharmaceutics
Medical Laboratory Technology
Abstract

Flow cytometry (FCM) is a sophisticated technique that works on the principle of light scattering and fluorescence emission by the specific fluorescent probe-labeled cells as they pass through a laser beam. It offers several unique advantages as it allows fast, relatively quantitative, multiparametric analysis of cell populations at the single cell level. In addition, it also enables physical sorting of the cells to separate the subpopulations based on different parameters. In this constantly evolving field, innovative technologies such as imaging FCM, mass cytometry and Raman FCM are being developed in order to address limitations of traditional FCM. This review explains the general principles, main applications and recent advances in the field of FCM.

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GOST Copy
Manohar S. M., Shah P., Nair A. Flow cytometry: principles, applications and recent advances // Bioanalysis. 2021. Vol. 13. No. 3. pp. 181-198.
GOST all authors (up to 50) Copy
Manohar S. M., Shah P., Nair A. Flow cytometry: principles, applications and recent advances // Bioanalysis. 2021. Vol. 13. No. 3. pp. 181-198.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.4155/bio-2020-0267
UR - https://doi.org/10.4155/bio-2020-0267
TI - Flow cytometry: principles, applications and recent advances
T2 - Bioanalysis
AU - Manohar, Sonal M
AU - Shah, Prachi
AU - Nair, Anusree
PY - 2021
DA - 2021/02/05
PB - Taylor & Francis
SP - 181-198
IS - 3
VL - 13
PMID - 33543666
SN - 1757-6180
SN - 1757-6199
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2021_Manohar,
author = {Sonal M Manohar and Prachi Shah and Anusree Nair},
title = {Flow cytometry: principles, applications and recent advances},
journal = {Bioanalysis},
year = {2021},
volume = {13},
publisher = {Taylor & Francis},
month = {feb},
url = {https://doi.org/10.4155/bio-2020-0267},
number = {3},
pages = {181--198},
doi = {10.4155/bio-2020-0267}
}
MLA
Cite this
MLA Copy
Manohar, Sonal M., et al. “Flow cytometry: principles, applications and recent advances.” Bioanalysis, vol. 13, no. 3, Feb. 2021, pp. 181-198. https://doi.org/10.4155/bio-2020-0267.