Journal of the American Chemical Society, volume 139, issue 25, pages 8766-8771

Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein

Svendsen Annette ¹

Kiefer H. V. ¹

Pedersen Henrik B ¹

Bochenkova Anastasia V. ²

Andersen L P ¹

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Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark |

Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia |

Publication type: Journal Article

Publication date: 2017-06-19

American Chemical Society (ACS)

Journal: Journal of the American Chemical Society

Quartile SCImago

Quartile WOS

Impact factor: 15

ISSN: 00027863, 15205126

DOI: 10.1021/jacs.7b04987

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General Chemistry

Catalysis

Biochemistry

Colloid and Surface Chemistry

Abstract

Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP. By the use of a new femtosecond pump-probe scheme, based on time-resolved action spectroscopy, we demonstrate that the isolated deprotonated GFP chromophore can be trapped in the first excited state when cooled to 100 K. The trapping is shown to last for 1.2 ns, which is long enough to establish conditions for fluorescence and consistent with calculated trapping barriers in the electronically excited state. Thus, GFP fluorescence is traced back to an intrinsic chromophore property, and by improving excited-state trapping, protein interactions enhance the molecular fluorescence.

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Nature Communications	Nature Communications, 2, 2.67% Nature Communications 2 publications, 2.67%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 2.67% International Journal of Molecular Sciences 2 publications, 2.67%
Journal of the American Chemical Society	Journal of the American Chemical Society, 2, 2.67% Journal of the American Chemical Society 2 publications, 2.67%
Journal of Physical Chemistry A	Journal of Physical Chemistry A, 2, 2.67% Journal of Physical Chemistry A 2 publications, 2.67%
Chemistry - An Asian Journal	Chemistry - An Asian Journal, 2, 2.67% Chemistry - An Asian Journal 2 publications, 2.67%
Journal of Physical Chemistry B	Journal of Physical Chemistry B, 2, 2.67% Journal of Physical Chemistry B 2 publications, 2.67%
Journal of Molecular Structure	Journal of Molecular Structure, 1, 1.33% Journal of Molecular Structure 1 publication, 1.33%
RSC Advances	RSC Advances, 1, 1.33% RSC Advances 1 publication, 1.33%
European Physical Journal D	European Physical Journal D, 1, 1.33% European Physical Journal D 1 publication, 1.33%
Applied Spectroscopy	Applied Spectroscopy, 1, 1.33% Applied Spectroscopy 1 publication, 1.33%
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Methods and Applications in Fluorescence	Methods and Applications in Fluorescence, 1, 1.33% Methods and Applications in Fluorescence 1 publication, 1.33%
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Chemical Reviews	Chemical Reviews, 1, 1.33% Chemical Reviews 1 publication, 1.33%
	1 2 3 4 5 6 7 8 9

Citations by publishers

	2 4 6 8 10 12 14 16 18 20
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 19, 25.33% Royal Society of Chemistry (RSC) 19 publications, 25.33%
American Chemical Society (ACS)	American Chemical Society (ACS), 16, 21.33% American Chemical Society (ACS) 16 publications, 21.33%
Wiley	Wiley, 12, 16% Wiley 12 publications, 16%
Elsevier	Elsevier, 7, 9.33% Elsevier 7 publications, 9.33%
Springer Nature	Springer Nature, 6, 8% Springer Nature 6 publications, 8%
American Institute of Physics (AIP)	American Institute of Physics (AIP), 3, 4% American Institute of Physics (AIP) 3 publications, 4%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 2, 2.67% Multidisciplinary Digital Publishing Institute (MDPI) 2 publications, 2.67%
IOP Publishing	IOP Publishing, 2, 2.67% IOP Publishing 2 publications, 2.67%
SAGE	SAGE, 1, 1.33% SAGE 1 publication, 1.33%
Frontiers Media S.A.	Frontiers Media S.A., 1, 1.33% Frontiers Media S.A. 1 publication, 1.33%
Pleiades Publishing	Pleiades Publishing, 1, 1.33% Pleiades Publishing 1 publication, 1.33%
Taylor & Francis	Taylor & Francis, 1, 1.33% Taylor & Francis 1 publication, 1.33%
American Physical Society (APS)	American Physical Society (APS), 1, 1.33% American Physical Society (APS) 1 publication, 1.33%
	2 4 6 8 10 12 14 16 18 20

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Svendsen A. et al. Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein // Journal of the American Chemical Society. 2017. Vol. 139. No. 25. pp. 8766-8771.

GOST all authors (up to 50) Copy

Svendsen A., Kiefer H. V., Pedersen H. B., Bochenkova A. V., Andersen L. P. Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein // Journal of the American Chemical Society. 2017. Vol. 139. No. 25. pp. 8766-8771.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1021/jacs.7b04987

UR - https://doi.org/10.1021%2Fjacs.7b04987

TI - Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein

T2 - Journal of the American Chemical Society

AU - Svendsen, Annette

AU - Bochenkova, Anastasia V.

AU - Andersen, L P

AU - Kiefer, H. V.

AU - Pedersen, Henrik B

PY - 2017

DA - 2017/06/19 00:00:00

PB - American Chemical Society (ACS)

SP - 8766-8771

IS - 25

VL - 139

SN - 0002-7863

SN - 1520-5126

ER -

BibTex |

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BibTex Copy

@article{2017_Svendsen,

author = {Annette Svendsen and Anastasia V. Bochenkova and L P Andersen and H. V. Kiefer and Henrik B Pedersen},

title = {Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein},

journal = {Journal of the American Chemical Society},

year = {2017},

volume = {139},

publisher = {American Chemical Society (ACS)},

month = {jun},

url = {https://doi.org/10.1021%2Fjacs.7b04987},

number = {25},

pages = {8766--8771},

doi = {10.1021/jacs.7b04987}

}

MLA

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MLA Copy

Svendsen, Annette, et al. “Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein.” Journal of the American Chemical Society, vol. 139, no. 25, Jun. 2017, pp. 8766-8771. https://doi.org/10.1021%2Fjacs.7b04987.

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Publisher

American Chemical Society (ACS)

Journal

Journal of the American Chemical Society

Quartile SCImago

Quartile WOS

Impact factor

ISSN

00027863 (Print)
15205126 (Electronic)

Labs

Laboratory of Quantum Photodynamics

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