Fungal Biology, volume 126, issue 1, pages 91-100
Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17β-hydroxysteroid dehydrogenase activities
Publication type: Journal Article
Publication date: 2022-01-01
Journal:
Fungal Biology
Quartile SCImago
Q2
Quartile WOS
Q3
Impact factor: 2.5
ISSN: 18786146, 21987777, 21987785
Genetics
Infectious Diseases
Ecology, Evolution, Behavior and Systematics
Abstract
Fungal strain Drechslera sp. Ph F-34 was shown to modify 3-oxo- and 3-hydroxy steroids of androstane series to form the corresponding allylic 7-alcohols and 17β-reduced derivatives thus evidencing the presence of 7α-, 7β-hydroxylase and 17β-hydroxysteroid dehydrogenase (17β-HSD) activities. The growing mycelium predominantly hydroxylated androsta-1,4-diene-3,17-dione (ADD) at the 7β-position, while much lower 7α-hydroxylation was observed. Along with 7β-hydroxy-ADD and its corresponding 7α-isomer, their respective 17β-alcohols were produced. In this study, transformation of ADD, androst-4-en-17β-ol-3-one (testosterone, TS) and 3β-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA) by resting mycelium of Drechslera sp. have been estimated in different conditions with regard to the inducibility and functionality of the 17β-HSD and 7-hydroxylase enzyme systems. Steroids of androstane, pregnane and cholane series were evaluated as inducers. The inhibitory analysis was provided using cycloheximide (CHX). Steroids were assayed using TLC and HPLC methods, and the structures were confirmed by mass-spectrometry, 1H and 13C NMR spectroscopy data. 17β-HSD of the mycelium constitutively reduced 17-carbonyl group of ADD and DHEA to form the corresponding 17β-alcohols, namely, androsta-1,4-diene-17β-ol-3-one (1-dehydro-TS), and androst-5-ene-3β,17β-diol. Production of the 7α- and 7β-hydroxylated derivatives depended on the induction conditions. The inducer effect relied on the steroid structure and decreased in the order: DHEA > pregnenolone > lithocholic acid. β-Sitosterol did not induce hydroxylase activity in Drechslera sp. CHX fully inhibited the synthesis of 7-hydroxylase in Drechslera mycelium thus providing selective 17-keto reduction. Results contribute to the diversity of steroid modifying enzymes in fungi and can be used at the development of novel biocatalysts for production of valuable steroid 7(α/β)- and 17β-alcohols.
Citations by journals
|
1
|
|
|
Microbiology Resource Announcements
|
Microbiology Resource Announcements
1 publication, 50%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
1 publication, 50%
|
|
1
|
Citations by publishers
|
1
|
|
|
American Society for Microbiology
|
American Society for Microbiology
1 publication, 50%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 50%
|
|
1
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2023],"ids":[0],"codes":[0],"imageUrls":[""],"datasets":[{"label":"Citations number","data":[2],"backgroundColor":["#3B82F6"],"percentage":["100"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Microbiology Resource Announcements","International Journal of Molecular Sciences"],"ids":[21864,14627],"codes":[0,0],"imageUrls":["\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp"],"datasets":[{"label":"","data":[1,1],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":[50,50],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["American Society for Microbiology","Multidisciplinary Digital Publishing Institute (MDPI)"],"ids":[142,202],"codes":[0,0],"imageUrls":["\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp"],"datasets":[{"label":"","data":[1,1],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":[50,50],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Kollerov V. et al. Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17β-hydroxysteroid dehydrogenase activities // Fungal Biology. 2022. Vol. 126. No. 1. pp. 91-100.
GOST all authors (up to 50)
Copy
Kollerov V., Shutov A. A., Kazantsev A., Donova M., Donova M. V. Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17β-hydroxysteroid dehydrogenase activities // Fungal Biology. 2022. Vol. 126. No. 1. pp. 91-100.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/j.funbio.2021.11.002
UR - https://doi.org/10.1016%2Fj.funbio.2021.11.002
TI - Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17β-hydroxysteroid dehydrogenase activities
T2 - Fungal Biology
AU - Kollerov, Vyacheslav
AU - Shutov, A. A.
AU - Kazantsev, Alexey
AU - Donova, Marina
AU - Donova, M. V.
PY - 2022
DA - 2022/01/01 00:00:00
PB - Elsevier
SP - 91-100
IS - 1
VL - 126
SN - 1878-6146
SN - 2198-7777
SN - 2198-7785
ER -
Cite this
BibTex
Copy
@article{2022_Kollerov,
author = {Vyacheslav Kollerov and A. A. Shutov and Alexey Kazantsev and Marina Donova and M. V. Donova},
title = {Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17β-hydroxysteroid dehydrogenase activities},
journal = {Fungal Biology},
year = {2022},
volume = {126},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016%2Fj.funbio.2021.11.002},
number = {1},
pages = {91--100},
doi = {10.1016/j.funbio.2021.11.002}
}
Cite this
MLA
Copy
Kollerov, Vyacheslav, et al. “Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17β-hydroxysteroid dehydrogenase activities.” Fungal Biology, vol. 126, no. 1, Jan. 2022, pp. 91-100. https://doi.org/10.1016%2Fj.funbio.2021.11.002.
Profiles