LIM kinase inhibitor T56-LIMKi protects mouse brain from photothrombotic stroke

Demyanenko S.V., Uzdensky A.
Тип документаJournal Article
Дата публикации2021-02-01
Название журнала
  • Clinical Neurology
  • Developmental and Educational Psychology
  • Neuroscience (miscellaneous)
Краткое описание
ABSTRACT Primary Objective: In an ischemic stroke, the damage spreads from the infarction core to surrounding tissues. The present work was aimed at the search of effective neuroprotectors that restrict injury propagation. Research Design: We studied possible protective effects of inhibitors of protein kinases LIMK2 (T56-LIMKi), DYRK1A (harmine), and tryptophan hydroxylase (4-chlorophenylalanine) on infarction size and morphology of peri-infarct area after photothrombotic stroke (a model of ischemic stroke) in mouse brain. Methods and Procedures: Photothrombotic stroke was induced by laser irradiation of mouse cortex after administration of photosensitizer Bengal Rose, which does not penetrate cells and remains in blood vessels. Under light exposure, it induces vessel occlusion. Infarct volume and histological changes in the cerebral cortex were evaluated 3, 7 and 14 days after photothrombotic impact. Main Outcomes and Results: Harmine and 4-chlorophenylalanine did not influence infarct volume and morphology of peri-infarct area in the mouse brain cortex after photothrombotic stroke. However, LIMK2 inhibitor T56-LIMKi significantly reduced infarct volume 7 and 14 days after photothrombotic stroke. It also increased the percent of normochromic neurons and decreased the fraction of altered cortical cells (hypochromic, hyperchromic and pyknotic neurons). Conclusions: T56-LIMK2i may be considered as a promising anti-stroke agent.
Пристатейные ссылки: 50
Kinematic Changes in a Mouse Model of Penetrating Hippocampal Injury and Their Recovery After Intranasal Administration of Endometrial Mesenchymal Stem Cell-Derived Extracellular Vesicles
León-Moreno L.C., Castañeda-Arellano R., Aguilar-García I.G., Desentis-Desentis M.F., Torres-Anguiano E., Gutiérrez-Almeida C.E., Najar-Acosta L.J., Mendizabal-Ruiz G., Ascencio-Piña C.R., Dueñas-Jiménez J.M., Rivas-Carrillo J.D., Dueñas-Jiménez S.H.
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Wei L., Li J., Tao S., Pan Y., Zhang Y., Jiang M.Q., Zachory Wei Z., Wang S., Zhu Y., Zhang C.
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Won S.J., Minnella A.M., Wu L., Eun C.H., Rome E., Herson P.S., Shaw A.E., Bamburg J.R., Swanson R.A.
Q1 PLoS ONE 2018 цитирований: 13
Brain ischemic insult induces cofilin rod formation leading to synaptic dysfunction in neurons
Shu L., Chen B., Chen B., Xu H., Wang G., Huang Y., Zhao Y., Gong H., Jiang M., Chen L., Liu X., Wang Y.
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Demyanenko S., Neginskaya M., Berezhnaya E.
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Photothrombotic Stroke as a Model of Ischemic Stroke
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Effect of anesthetics on efficiency of remote ischemic preconditioning
Silachev D.N., Usatikova E.A., Pevzner I.B., Zorova L.D., Babenko V.A., Gulyaev M.V., Pirogov Y.A., Plotnikov E.Y., Zorov D.B.
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Experimental neuroprotection in ischemic stroke: a concise review
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LIM kinases: cofilin and beyond
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Q1 Oncotarget 2017 цитирований: 46
Hankey G.J.
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Ischemic stroke: experimental models and reality
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1. Demyanenko S.V., Uzdensky A. LIM kinase inhibitor T56-LIMKi protects mouse brain from photothrombotic stroke // Brain Injury. 2021. Т. 35. № 4. С. 490–500.


DO - 10.1080/02699052.2021.1879397

UR -

TI - LIM kinase inhibitor T56-LIMKi protects mouse brain from photothrombotic stroke

T2 - Brain Injury

AU - Demyanenko, Svetlana V.

AU - Uzdensky, Anatoly

PY - 2021

DA - 2021/02/01

PB - Informa UK Limited

SP - 490-500

IS - 4

VL - 35

SN - 0269-9052

SN - 1362-301X

ER -

BibTex |


doi = {10.1080/02699052.2021.1879397},

url = {},

year = 2021,

month = {feb},

publisher = {Informa {UK} Limited},

volume = {35},

number = {4},

pages = {490--500},

author = {Svetlana V. Demyanenko and Anatoly Uzdensky},

title = {{LIM} kinase inhibitor T56-{LIMKi} protects mouse brain from photothrombotic stroke},

journal = {Brain Injury}


Demyanenko, Svetlana V., and Anatoly Uzdensky. “LIM Kinase Inhibitor T56-LIMKi Protects Mouse Brain from Photothrombotic Stroke.” Brain Injury 35.4 (2021): 490–500. Crossref. Web.