Open Access
Biomedicines, volume 9, issue 11, pages 1741
Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
Kashirina Alena
1
,
Gavrina Alena
1
,
Kryukov Emil
1
,
Elagin Vadim
1
,
Kolesova Yuliya
2, 3
,
Artyuhov Alexander
4
,
Momotyuk Ekaterina
4
,
Abdyyev Vepa
3
,
Meshcheryakova Natalia
4
,
Zagaynova Elena
1, 5
,
Dashinimaev Erdem
3, 4, 6
,
Kashina Aleksandra
1
1
Publication type: Journal Article
Publication date: 2021-11-22
PubMed ID:
34829971
General Biochemistry, Genetics and Molecular Biology
Medicine (miscellaneous)
Abstract
Brain diseases including Down syndrome (DS/TS21) are known to be characterized by changes in cellular metabolism. To adequately assess such metabolic changes during pathological processes and to test drugs, methods are needed that allow monitoring of these changes in real time with minimally invasive effects. Thus, the aim of our work was to study the metabolic status and intracellular pH of spheroids carrying DS using fluorescence microscopy and FLIM. For metabolic analysis we measured the fluorescence intensities, fluorescence lifetimes and the contributions of the free and bound forms of NAD(P)H. For intracellular pH assay we measured the fluorescence intensities of SypHer-2 and BCECF. Data were processed with SPCImage and Fiji-ImageJ. We demonstrated the predominance of glycolysis in TS21 spheroids compared with normal karyotype (NK) spheroids. Assessment of the intracellular pH indicated a more alkaline intracellular pH in the TS21 spheroids compared to NK spheroids. Using fluorescence imaging, we performed a comprehensive comparative analysis of the metabolism and intracellular pH of TS21 spheroids and showed that fluorescence microscopy and FLIM make it possible to study living cells in 3D models in real time with minimally invasive effects.
Citations by journals
|
1
|
|
|
Cells
|
Cells
1 publication, 50%
|
|
Cellular and Molecular Life Sciences
|
Cellular and Molecular Life Sciences
1 publication, 50%
|
|
1
|
Citations by publishers
|
1
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 50%
|
|
Springer Nature
|
Springer Nature
1 publication, 50%
|
|
1
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2022,2023,2024],"ids":[0,0,0],"codes":[0,0,0],"imageUrls":["","",""],"datasets":[{"label":"Citations number","data":[1,0,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":["50",0,"50"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Cells","Cellular and Molecular Life Sciences"],"ids":[25293,5151],"codes":[0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[1,1],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":[50,50],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Springer Nature"],"ids":[202,8],"codes":[0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[1,1],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":[50,50],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Kashirina A. et al. Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome // Biomedicines. 2021. Vol. 9. No. 11. p. 1741.
GOST all authors (up to 50)
Copy
Kashirina A., Gavrina A., Kryukov E., Elagin V., Kolesova Y., Artyuhov A., Momotyuk E., Abdyyev V., Meshcheryakova N., Zagaynova E., Dashinimaev E., Kashina A. Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome // Biomedicines. 2021. Vol. 9. No. 11. p. 1741.
Cite this
RIS
Copy
TY - JOUR
DO - 10.3390/biomedicines9111741
UR - https://doi.org/10.3390%2Fbiomedicines9111741
TI - Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
T2 - Biomedicines
AU - Artyuhov, Alexander
AU - Momotyuk, Ekaterina
AU - Meshcheryakova, Natalia
AU - Dashinimaev, Erdem
AU - Zagaynova, Elena
AU - Kashirina, Alena
AU - Gavrina, Alena
AU - Kashina, Aleksandra
AU - Kryukov, Emil
AU - Elagin, Vadim
AU - Kolesova, Yuliya
AU - Abdyyev, Vepa
PY - 2021
DA - 2021/11/22 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1741
IS - 11
VL - 9
PMID - 34829971
SN - 2227-9059
ER -
Cite this
BibTex
Copy
@article{2021_Kashirina,
author = {Alexander Artyuhov and Ekaterina Momotyuk and Natalia Meshcheryakova and Erdem Dashinimaev and Elena Zagaynova and Alena Kashirina and Alena Gavrina and Aleksandra Kashina and Emil Kryukov and Vadim Elagin and Yuliya Kolesova and Vepa Abdyyev},
title = {Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome},
journal = {Biomedicines},
year = {2021},
volume = {9},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390%2Fbiomedicines9111741},
number = {11},
pages = {1741},
doi = {10.3390/biomedicines9111741}
}
Cite this
MLA
Copy
Kashirina, Alena, et al. “Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome.” Biomedicines, vol. 9, no. 11, Nov. 2021, p. 1741. https://doi.org/10.3390%2Fbiomedicines9111741.