Open Access
PLoS ONE, volume 16, issue 11, pages e0260727
The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension
Publication type: Journal Article
Publication date: 2021-11-30
PubMed ID:
34847207
Multidisciplinary
Abstract
The actin cytoskeleton is indispensable for the motility and migration of all types of cells; therefore, it plays a crucial role in the ability of the tissues to repair. Mesenchymal stem cells are intensively used in regenerative medicine, but usually relatively low percent of transplanted cells reaches the injury. To overcome this evident limitation, researchers try to enhance the motility and migration rate of the cells. As one of the approaches, co-cultivation and preconditioning of stem cells with biologically active compounds, which can cause actin cytoskeleton rearrangements followed by an increase of migratory properties of the cells, could be applied. The observed changes in F-actin structure induced by the compounds require quantitative estimation, and measurement of fluorescence intensity of the F-actin image captured by various microscopic techniques is commonly used nowadays. However, this approach could not always accurately detect the observed changes in the shape and structure of actin cytoskeleton. At this time, the image of F-actin has an irregular geometric pattern, and thus could be considered and characterized as a fractal object. To quantify the re-organization of cellular F-actin in terms of fractal geometry Minkovsky’s box-counting method is suitable, but it is not widely used nowadays. We modified and improved the previously described method for fractal dimension measurement, and successfully applied it for the quantification of the F-actin structures of human mesenchymal stem cells.
Citations by journals
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Cellular and Molecular Biology Letters
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Cellular and Molecular Biology Letters
1 publication, 11.11%
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Nano Express
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1 publication, 11.11%
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Biophysica
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Russian Journal of Bioorganic Chemistry
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1 publication, 11.11%
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Frontiers in Cell and Developmental Biology
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1 publication, 11.11%
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Journal of Ethnopharmacology
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Journal of Ethnopharmacology
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1
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Citations by publishers
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2
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 22.22%
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Pleiades Publishing
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Pleiades Publishing
2 publications, 22.22%
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Springer Nature
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Springer Nature
1 publication, 11.11%
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Wiley
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Wiley
1 publication, 11.11%
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IOP Publishing
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IOP Publishing
1 publication, 11.11%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 11.11%
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Elsevier
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Elsevier
1 publication, 11.11%
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1
2
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- Statistics recalculated weekly.
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Revittser A. et al. The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension // PLoS ONE. 2021. Vol. 16. No. 11. p. e0260727.
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Revittser A., Selin I., Negulyaev Y., Chubinskiy Nadezhdin V. The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension // PLoS ONE. 2021. Vol. 16. No. 11. p. e0260727.
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TY - JOUR
DO - 10.1371/journal.pone.0260727
UR - https://doi.org/10.1371%2Fjournal.pone.0260727
TI - The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension
T2 - PLoS ONE
AU - Revittser, Alla
AU - Selin, Ivan
AU - Negulyaev, Yuri
AU - Chubinskiy Nadezhdin, Vladislav
PY - 2021
DA - 2021/11/30 00:00:00
PB - Public Library of Science (PLoS)
SP - e0260727
IS - 11
VL - 16
PMID - 34847207
SN - 1932-6203
ER -
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@article{2021_Revittser,
author = {Alla Revittser and Ivan Selin and Yuri Negulyaev and Vladislav Chubinskiy Nadezhdin},
title = {The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension},
journal = {PLoS ONE},
year = {2021},
volume = {16},
publisher = {Public Library of Science (PLoS)},
month = {nov},
url = {https://doi.org/10.1371%2Fjournal.pone.0260727},
number = {11},
pages = {e0260727},
doi = {10.1371/journal.pone.0260727}
}
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MLA
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Revittser, Alla, et al. “The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension.” PLoS ONE, vol. 16, no. 11, Nov. 2021, p. e0260727. https://doi.org/10.1371%2Fjournal.pone.0260727.
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