Open Access
Polymers, volume 13, issue 22, pages 3889
Hydrogel Droplet Microarray for Genotyping Antimicrobial Resistance Determinants in Neisseria gonorrhoeae Isolates
Shaskolskiy B. L.
1
,
Kandinov Ilya
1
,
Kravtsov Dmitry
1
,
Vinokurova Alexandra
1
,
Gorshkova Sofya
1
,
Filippova Marina
1
,
Kubanov Alexey
2
,
Solomka Victoria
2
,
Deryabin Dmitry
2
,
DEMENTIEVA Ekaterina
1
,
Publication type: Journal Article
Publication date: 2021-11-10
PubMed ID:
34833187
General Chemistry
Polymers and Plastics
Abstract
A multiplex assay based on a low-density hydrogel microarray was developed to identify genomic substitutions in N. gonorrhoeae that determine resistance to the currently recommended treatment agents ceftriaxone and azithromycin and the previously used drugs penicillin, tetracycline, and ciprofloxacin. The microarray identifies 74 drug resistance determinants in the N. gonorrhoeae penA, ponA, porB, gyrA, parC, rpsJ, mtrR, blaTEM, tetM, and 23S rRNA genes. The hydrogel elements were formed by automated dispensing of nanoliter-volume droplets followed by UV-induced copolymerization of NH2-containing oligonucleotides with gel-forming monomers. Polybutylene terephthalate plates without special modifications were used as microarray substrates. Sequences and concentrations of immobilized oligonucleotides, gel composition, and hybridization conditions were carefully selected, and the median discrimination ratio ranged from 2.8 to 29.4, allowing unambiguous identification of single-nucleotide substitutions. The mutation identification results in a control sample of 180 N. gonorrhoeae isolates were completely consistent with the Sanger sequencing results. In total, 648 clinical N. gonorrhoeae isolates obtained in Russia during the last 5 years were analyzed and genotyped using these microarrays. The results allowed us to draw conclusions about the present situation with antimicrobial susceptibility of N. gonorrhoeae in Russia and demonstrated the possibility of using hydrogel microarrays to control the spread of antibiotic resistance.
Citations by journals
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1
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Pathogens
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Pathogens
1 publication, 20%
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Microorganisms
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1 publication, 20%
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Klinicheskaya Dermatologiya i Venerologiya
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1 publication, 20%
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
1 publication, 20%
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Current Issues in Molecular Biology
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Current Issues in Molecular Biology
1 publication, 20%
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1
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Citations by publishers
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1
2
3
4
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
4 publications, 80%
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Media Sphere Publishing Group
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Media Sphere Publishing Group
1 publication, 20%
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1
2
3
4
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Shaskolskiy B. L. et al. Hydrogel Droplet Microarray for Genotyping Antimicrobial Resistance Determinants in Neisseria gonorrhoeae Isolates // Polymers. 2021. Vol. 13. No. 22. p. 3889.
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Shaskolskiy B. L., Kandinov I., Kravtsov D., Vinokurova A., Gorshkova S., Filippova M., Kubanov A., Solomka V., Deryabin D., DEMENTIEVA E., Gryadunov D. A. Hydrogel Droplet Microarray for Genotyping Antimicrobial Resistance Determinants in Neisseria gonorrhoeae Isolates // Polymers. 2021. Vol. 13. No. 22. p. 3889.
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TY - JOUR
DO - 10.3390/polym13223889
UR - https://doi.org/10.3390%2Fpolym13223889
TI - Hydrogel Droplet Microarray for Genotyping Antimicrobial Resistance Determinants in Neisseria gonorrhoeae Isolates
T2 - Polymers
AU - Vinokurova, Alexandra
AU - Kubanov, Alexey
AU - Solomka, Victoria
AU - DEMENTIEVA, Ekaterina
AU - Kravtsov, Dmitry
AU - Deryabin, Dmitry
AU - Filippova, Marina
AU - Shaskolskiy, B. L.
AU - Kandinov, Ilya
AU - Gorshkova, Sofya
AU - Gryadunov, D. A.
PY - 2021
DA - 2021/11/10 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 3889
IS - 22
VL - 13
PMID - 34833187
SN - 2073-4360
ER -
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@article{2021_Shaskolskiy,
author = {Alexandra Vinokurova and Alexey Kubanov and Victoria Solomka and Ekaterina DEMENTIEVA and Dmitry Kravtsov and Dmitry Deryabin and Marina Filippova and B. L. Shaskolskiy and Ilya Kandinov and Sofya Gorshkova and D. A. Gryadunov},
title = {Hydrogel Droplet Microarray for Genotyping Antimicrobial Resistance Determinants in Neisseria gonorrhoeae Isolates},
journal = {Polymers},
year = {2021},
volume = {13},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390%2Fpolym13223889},
number = {22},
pages = {3889},
doi = {10.3390/polym13223889}
}
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Shaskolskiy, B. L., et al. “Hydrogel Droplet Microarray for Genotyping Antimicrobial Resistance Determinants in Neisseria gonorrhoeae Isolates.” Polymers, vol. 13, no. 22, Nov. 2021, p. 3889. https://doi.org/10.3390%2Fpolym13223889.