Plant Genetic Engineering Laboratory (PGEL)
Publications
139
Citations
2 451
h-index
27
Authorization required.
Our lab specializes in cutting-edge research at the intersection of plant biotechnology and molecular biology. With a focus on Plant Metabolic Engineering, we aim to enhance the biosynthesis of high-value secondary metabolites in medicinal plants. Our expertise in Plant DNA Barcoding enables accurate identification and phylogenetic studies, while Plant Molecular Farming explores the production of pharmaceutical and industrial compounds using plants as biofactories. Through innovative approaches, we contribute to sustainable and impactful advancements in plant science.
- 1. Microbiological methods and cultural methods. Search, isolation, description and study of aerobic and anaerobic bioindicator microorganisms associated with abnormal gas fields and atutigenic mineralization. Work with living pure and accumulative cultures of methanotrophic, methylotrophic, hydrocarbon-oxidizing and sulfate-reducing microorganisms. Molecular genetic methods. Analysis of 16S rDNA and functional genes by PC-real time method.
- Agrobacterial transformation of plants
- Bioinformatic methods
- Cloning GATEWAY
- Isolation and purification of target proteins
- Methods for obtaining adventitious roots, callus and suspension cell cultures of higher plants. Methods for assessing the respiratory metabolism of suspension cultures of higher plant cells
- Methods of qualitative and quantitative assessment of the content of secondary metabolites in cultures of cells and organs of higher plants (HPLC, NMR, HPLC-MS, spectrophotometry)
- Microbiological methods (Methods of Microbiology)
- Molecular biology of plants (Plant Molecular Biology)
- Obtaining bacterial and eukaryotic vectors using genetically engineered approaches
- Real-time PCR (qPCR)
- Real-time PCR and digital PCR, including design and validation of primers and probes to your target
- Recombinant protein expression
- CRISPR/Cas9-mediated editing of plant genomes
Sathishkumar Ramalingam
Head of Laboratory
Parthiban Subramanian
PhD student
Vijeesh Thandarvalli
PhD student
Gayathri Thashanamoorthi
PhD student
Hari Priya Sivakumar
PhD student
Abdul Basith Akbar Aly
PhD student
Sreelekshmi V. A.
PhD student
Research directions
Plant Metabolic Engineering
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Our lab specializes in the biofortification of crop plants through metabolic pathway engineering, resulting in increased levels of essential nutrients and highly valuable beneficial compounds. Our successful projects include enhancing Vitamin E in tomatoes and rice, genistein in onions, and carnosol/carnosic acid in garden sage through the manipulation of biosynthesis pathways. Our research has been published in esteemed journals such as the Plant Biotechnology Journal, Plants, and Biologia Plantarum, etc. This work is supported by funding from the Department of Biotechnology (DBT) and Bharathiar University-Defence Research and Development Organisation for Life Sciences (BU-DRDO).
Plant DNA Barcoding
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Our lab specializes in the application of DNA barcoding techniques, which have become an essential tool for authenticating medicinal plants, herbal products, and identifying the botanical origins of honey samples. Our work is supported by DBT-Twinning funding and is being conducted in collaboration with Dr. Steven Newmaster, Director of the NHP Alliance at the University of Guelph, Canada. Additionally, we provide consultancy services to renowned herbal industries, ensuring the accuracy and authenticity of their plant-based products. Our research has been featured in prominent journals such as BMC Medicine, Drug Safety, and Genome, underscoring the impact and reliability of our methodologies in the field of plant authentication.
Plant Molecular Farming
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Our lab has successfully developed a robust plant expression platform, enabling the production of Chikungunya antigens and antibodies. Building on this success, we are currently focused on generating diagnostic reagents for Dengue and Kyasanur Forest Disease (KFD), as well as biomarkers for cancer detection. Our groundbreaking work is published in esteemed journals such as the International Journal of Infectious Diseases and Plant Biotechnology. This research is generously funded by UKIERI, RUSA, and TANSCHE and is being conducted in collaboration with Prof. Julian Ma, Director of the Institute for Infection and Immunity at St. George’s University of London.
Publications and patents
Found
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Meleen Chye, Hongye Li, Ramalingam Sathishkumar, Litman Leo Poon, Sriyal Malik Joseph Peiris
WO2005054473A1,
2005
Partners
St George's University of London
University of Évora
Lab address
Plant Genetic Engineering Laboratory (PGEL), Department of Biotechnology, Bharathiar University, Coimbatore - 641046, Tamil Nadu, India.
Authorization required.