Laboratory of Cell and Tissue Growth

Development site panacerium.com

The mitogenic effect of cerium (IV) oxide and cerium (III) fluoride nanoparticles on the regeneration of the whole organism - freshwater flatworms Schmidtea mediterranea (planarium). Both types of cerium-containing nanoparticles are very powerful mitogens for planarians. CeO2 and CeF3 in micro- and nanomolar concentrations significantly accelerate the growth of planar blastema by increasing cell proliferation, causing an increase in the mitotic index and the number of blastema cells in regenerating planaria. CeO2 provides maximum activity at concentrations that are two orders of magnitude lower than those of CeF3. The valence state of cerium in cerium-containing nanoparticles plays a significant role in the mechanism of planarian regeneration: CeO2 nanoparticles, containing mainly Ce4+ particles, presumably absorb reactive oxygen species from the wound and moderately activate gene expression processes, whereas the regenerative effect of CeF3 nanoparticles containing only Ce3+ species is manifested in the pronounced expression of genes involved in division, differentiation and cell migration.

In this work, we have shown the possibility of effective encapsulation of luciferase in biodegradable polyelectrolyte microcapsules modified with therapeutically active cerium dioxide nanoparticles. It has been shown that the modification of microcapsules with citrate-stabilized cerium dioxide nanoparticles ensures the preservation of high activity of the encapsulated protein when exposed to a damaging agent - hydrogen peroxide. This structure of the microcapsule makes it possible to ensure the safety of the encapsulated substance in aggressive environmental conditions, as well as to ensure its prolonged release from it. The use of redox-active nanoparticles in the structure of microcapsules will allow the creation of new diagnostic and therapeutic drugs for the effective treatment and diagnosis of a wide range of socially significant diseases.