skill

GoldenGate cloning

Volkov Pavel
🥼 🤝
PhD in Chemistry, Associate Professor
MISiS
MISiS
Research Center of Biotechnology RAS
Research Center of Biotechnology RAS
18 publications,  275 citations
h-index: 10
Skills
Affinity chromatography
Gel electrophoresis
PCR
Spectrophotometry
Gel penetrating chromatography
Purification of nucleic acids
Site-directed mutagenesis
Analysis of enzyme activity
Working with polysaccharides: isolation, study, obtaining structures
Preparation of chemically competent E. coli cells
Sequencing
DNA extraction
GoldenGate cloning
The mechanism of action of the enzyme on the substrate
Plasmid DNA extraction
Kiryushkin Alexey
BIN RAS
BIN RAS
11 publications,  84 citations
h-index: 5
Skills
Gel electrophoresis
Confocal laser scanning microscopy
PCR
Real-time PCR (qPCR)
Fluorescence microscopy
Molecular cloning
RNA extraction
Preparation of chemically competent E. coli cells
DNA extraction
GATEWAY cloning
Agrobacterial transformation of plants
Preparation of electrocompetent cells of Agrobacterium rhizogenes/tumefaciens
RNA interference
Overexpression
CRISPR/Cas9-mediated editing of plant genomes
GoldenGate cloning