skill

Site-directed mutagenesis

Boldinova Elizaveta
🥼
PhD in Biological/biomedical sciences
IGB RAS
IGB RAS
IMG NRC KI
IMG NRC KI
NRC KI
NRC KI
19 publications,  127 citations
h-index: 6
Skills
Affinity chromatography
Isolation and purification of target proteins
Gel electrophoresis
PCR
Recombinant protein expression
CRISPR-Cas
Molecular cloning
Site-directed mutagenesis
In vitro testing of DNA polymerase activity
Volkov Pavel
🥼 🤝
PhD in Chemistry, Associate Professor
MISiS
MISiS
Research Center of Biotechnology RAS
Research Center of Biotechnology RAS
18 publications,  274 citations
h-index: 10
Skills
Affinity chromatography
Gel electrophoresis
PCR
Spectrophotometry
Gel penetrating chromatography
Purification of nucleic acids
Site-directed mutagenesis
Analysis of enzyme activity
Working with polysaccharides: isolation, study, obtaining structures
Preparation of chemically competent E. coli cells
Sequencing
DNA extraction
GoldenGate cloning
The mechanism of action of the enzyme on the substrate
Plasmid DNA extraction