skill

Working with polysaccharides: isolation, study, obtaining structures

Volkov Pavel
🥼 🤝
PhD in Chemistry, Associate Professor
MISiS
MISiS
Research Center of Biotechnology RAS
Research Center of Biotechnology RAS
18 publications,  275 citations
h-index: 10
Skills
Affinity chromatography
Gel electrophoresis
PCR
Spectrophotometry
Gel penetrating chromatography
Purification of nucleic acids
Site-directed mutagenesis
Analysis of enzyme activity
Working with polysaccharides: isolation, study, obtaining structures
Preparation of chemically competent E. coli cells
Sequencing
DNA extraction
GoldenGate cloning
The mechanism of action of the enzyme on the substrate
Plasmid DNA extraction
Volkova Marina
🤝
PhD in Biological/biomedical sciences
8 publications,  19 citations
h-index: 3
Skills
Western blot
Enzyme-linked immunosorbent assay (ELISA)
Working with laboratory animals
MALDI mass spectroscopy
Python
Primary cell culture
3D-spheroids
ESI-MS mass spectroscopy
Cultivation of eukaryotic cells
Working with prokaryotic cells: cultivation, transformation
Surgical operations on animals
Working with polysaccharides: isolation, study, obtaining structures