skill

Preparation of chemically competent E. coli cells

Orlova Elizaveta
🤝 🥼
SCFHHRP
SCFHHRP
25 publications,  42 citations
h-index: 4
Skills
Western blot
Gel electrophoresis
Enzyme-linked immunosorbent assay (ELISA)
CRISPR-Cas
Primer Design
Optical microscopy
Molecular cloning
Purification of nucleic acids
Cultivation of eukaryotic cells
Working with prokaryotic cells: cultivation, transformation
Preparation of chemically competent E. coli cells
Sequencing
DNA extraction
Efimova Kseniya
PhD in Biological/biomedical sciences
NSCMB FEB RAS
NSCMB FEB RAS
21 publications,  137 citations
h-index: 7
Skills
Gel electrophoresis
PCR
Sanger sequencing
Next Generation Sequencing (NGS)
RT-qPCR
Molecular cloning
Bioinformatics
Genotyping
Preparation of chemically competent E. coli cells
screening of polyketide synthase (PKS) synthesis genes, monitoring of potentially dangerous and biotechnologically valuable species/strains of microorganisms.
Sequencing
DNA extraction
Whole genome sequencing (WGS)
Volkov Pavel
🥼 🤝
PhD in Chemistry, Associate Professor
MISiS
MISiS
Research Center of Biotechnology RAS
Research Center of Biotechnology RAS
18 publications,  275 citations
h-index: 10
Skills
Affinity chromatography
Gel electrophoresis
PCR
Spectrophotometry
Gel penetrating chromatography
Purification of nucleic acids
Site-directed mutagenesis
Analysis of enzyme activity
Working with polysaccharides: isolation, study, obtaining structures
Preparation of chemically competent E. coli cells
Sequencing
DNA extraction
GoldenGate cloning
The mechanism of action of the enzyme on the substrate
Plasmid DNA extraction
Perfilov Maxim
🥼 🤝
IBCh RAS
IBCh RAS
13 publications,  58 citations
h-index: 5
Skills
Gel electrophoresis
PCR
Fluorescence microscopy
Python
Molecular cloning
Fluorescent nanoscopy
Preparation of chemically competent E. coli cells
Sequencing
DNA extraction
Management and transfection of mammalian cell cultures
Kiryushkin Alexey
BIN RAS
BIN RAS
11 publications,  84 citations
h-index: 5
Skills
Gel electrophoresis
Confocal laser scanning microscopy
PCR
Real-time PCR (qPCR)
Fluorescence microscopy
Molecular cloning
RNA extraction
Preparation of chemically competent E. coli cells
DNA extraction
GATEWAY cloning
Agrobacterial transformation of plants
Preparation of electrocompetent cells of Agrobacterium rhizogenes/tumefaciens
RNA interference
Overexpression
CRISPR/Cas9-mediated editing of plant genomes
GoldenGate cloning
Salamaikina Svetlana
MIPT
MIPT
CRIE
CRIE
6 publications,  33 citations
h-index: 4
Skills
Sequence alignment
High performance liquid chromatography (HPLC)
Gel electrophoresis
Cloning
Cell and tissue culture
Pyrosequencing
PCR
Extraction
BLAST
UGENE
CorelDraw
Primer Design
RT-qPCR
Development of test systems
LaTex
Lens
Molecular biology experiment design
Molecular cloning
Purification of nucleic acids
Scientific writing
Associative research
In vitro testing of DNA polymerase activity
Adobe Illustrator
SMM
Genotyping
ChemDraw
Preparation of chemically competent E. coli cells
HRM
Sequencing
DNA extraction
Excel
Zotero
Butenko Anastasiya
🔍
Pirogov RNRMU
Pirogov RNRMU
h-index: 0
Skills
Isolation and purification of target proteins
Gel electrophoresis
Confocal laser scanning microscopy
PCR
R (programming language)
Gel penetrating chromatography
Spectroscopic methods
Cultivation of eukaryotic cells
Preparation of chemically competent E. coli cells
DNA extraction
Velichko Vera
🔍
h-index: 0
Skills
Gel electrophoresis
Cell and tissue culture
PCR
Real-time PCR (qPCR)
NMR spectroscopy
Next Generation Sequencing (NGS)
Brewster Angle Microscopy
Gel penetrating chromatography
Purification of nucleic acids
3D printing
Preparation of chemically competent E. coli cells
Sequencing
Chromatography
Determination of the rhesus of the fetus
NIPT
VILLAGE
DNA extraction
Cell cultivation
Vlaskina Olga
Pirogov RNRMU
Pirogov RNRMU
h-index: 0
Skills
PCR
UV-vis absorption spectroscopy
Transformation
Fluorescence microscopy
Fluorescence spectroscopy
R (programming language)
ImageJ
Lumine
Cultivation of eukaryotic cells
Spectroscopy
English (C1)
Preparation of chemically competent E. coli cells
Reading a course of lectures
Belousov Michael
🔍
NRU HSE
NRU HSE
h-index: 0
Skills
Affinity chromatography
Gel electrophoresis
PCR
Working with prokaryotic cells: cultivation, transformation, working with phages
NumPy
Pandas
Python
EMSA
Preparation of chemically competent E. coli cells
Protein electrophoresis
Double gel electrophoresis
Potapova Daryya
🤝
TSU
TSU
PBC RAS
PBC RAS
h-index: 0
Skills
High performance liquid chromatography (HPLC)
Gel electrophoresis
Spectrophotometry
Molecular cloning
Purification of nucleic acids
Preparation of chemically competent E. coli cells
Sequencing
DNA extraction
Cell cultivation
Yakuhina Valeriya
🔍
h-index: 0
Skills
Gel electrophoresis
PCR
Work with eukaryotic cells: cultivation, transfection, infection
Python
English
Colloidal chemistry
Preparation of chemically competent E. coli cells
DNA extraction
Scientific research
Plasmid DNA extraction
Dunaeva Anastasiya
ITMO University
ITMO University
h-index: 0
Skills
Western blot
Gel electrophoresis
PCR
Transfection
MTT
Cultivation of eukaryotic cells
Sowing of bacterial and fungal crops
Preparation of chemically competent E. coli cells
DNA extraction
Maslakova Angelina
ICG SB RAS
ICG SB RAS
NSU
NSU
h-index: 0
Skills
Gel electrophoresis
Cloning
PCR
Sanger sequencing
Python
R (programming language)
Preparation of chemically competent E. coli cells
DNA extraction
Preparation of electrocompetent cells of Agrobacterium rhizogenes/tumefaciens
Tagirova Alina
🔍
MSU
MSU
h-index: 0
Skills
Western blot
Gel electrophoresis
PCR
Sanger sequencing
Fluorescence microscopy
BLAST
CRISPR-Cas
Luminescence spectroscopy
RT-qPCR
RStudio
Molecular cloning
RNA-Seq Data Analysis
Purification of nucleic acids
Genomic editing
RNA extraction
Preparation of chemically competent E. coli cells
Immunofluorescence
DNA extraction