Angewandte Chemie - International Edition

, volume 62 , issue 25

Genetic Fusion of Thermoresponsive Polypeptides with UCST‐type Behavior Mediates 1D Assembly of Coiled‐Coil Bundlemers

Sai S Patkar ¹

Yao Tang ¹

Arriana M. Bisram ¹

Tianren Zhang ^{1, 2}

Jeffery G. Saven ²

Darrin P. Pochan ¹

Kristi Kiick ^{1, 3}

Hide authors affiliations Show authors affiliations: 3 affiliations

Department of Materials Science and Engineering University of Delaware Newark DE 19716 USA |

Department of Chemistry University of Pennsylvania Philadelphia PA 19104 USA |

Department of Biomedical Engineering University of Delaware Newark DE 19716 USA |

Publication type: Journal Article

Publication date: 2023-05-09

Wiley

Angewandte Chemie - International Edition

scimago Q1

wos Q1

SJR: 5.550

CiteScore: 27.6

Impact factor: 16.9

ISSN: 14337851, 15213773

DOI: 10.1002/anie.202301331

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PubMed ID: 36988077

General Chemistry

Catalysis

Abstract

Thermoresponsive resilin‐like polypeptides (RLPs) of various lengths were genetically fused to two different computationally designed coiled coil‐forming peptides with distinct thermal stability, to develop new strategies to assemble coiled coil peptides via temperature‐triggered phase separation of the RLP units. Their successful production in bacterial expression hosts was verified via gel electrophoresis, mass spectrometry, and amino acid analysis. Circular dichroism (CD) spectroscopy, ultraviolet‐visible (UV/Vis) turbidimetry, and dynamic light scattering (DLS) measurements confirmed the stability of the coiled coils and showed that the thermosensitive phase behavior of the RLPs was preserved in the genetically fused hybrid polypeptides. Cryogenic‐transmission electron microscopy and coarse‐grained modeling revealed that functionalizing the coiled coils with thermoresponsive RLPs leads to their thermally triggered noncovalent assembly into nanofibrillar assemblies.

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GOST Copy

Patkar S. S. et al. Genetic Fusion of Thermoresponsive Polypeptides with UCST‐type Behavior Mediates 1D Assembly of Coiled‐Coil Bundlemers // Angewandte Chemie - International Edition. 2023. Vol. 62. No. 25.

GOST all authors (up to 50) Copy

Patkar S. S., Tang Y., Bisram A. M., Zhang T., Saven J. G., Pochan D. P., Kiick K. Genetic Fusion of Thermoresponsive Polypeptides with UCST‐type Behavior Mediates 1D Assembly of Coiled‐Coil Bundlemers // Angewandte Chemie - International Edition. 2023. Vol. 62. No. 25.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1002/anie.202301331

UR - https://doi.org/10.1002/anie.202301331

TI - Genetic Fusion of Thermoresponsive Polypeptides with UCST‐type Behavior Mediates 1D Assembly of Coiled‐Coil Bundlemers

T2 - Angewandte Chemie - International Edition

AU - Patkar, Sai S

AU - Tang, Yao

AU - Bisram, Arriana M.

AU - Zhang, Tianren

AU - Saven, Jeffery G.

AU - Pochan, Darrin P.

AU - Kiick, Kristi

PY - 2023

DA - 2023/05/09

PB - Wiley

IS - 25

VL - 62

PMID - 36988077

SN - 1433-7851

SN - 1521-3773

ER -

BibTex

Cite this

BibTex (up to 50 authors) Copy

@article{2023_Patkar,

author = {Sai S Patkar and Yao Tang and Arriana M. Bisram and Tianren Zhang and Jeffery G. Saven and Darrin P. Pochan and Kristi Kiick},

title = {Genetic Fusion of Thermoresponsive Polypeptides with UCST‐type Behavior Mediates 1D Assembly of Coiled‐Coil Bundlemers},

journal = {Angewandte Chemie - International Edition},

year = {2023},

volume = {62},

publisher = {Wiley},

month = {may},

url = {https://doi.org/10.1002/anie.202301331},

number = {25},

doi = {10.1002/anie.202301331}

}

Publisher

Wiley

Journal

Angewandte Chemie - International Edition

scimago Q1

wos Q1

SJR

5.550

CiteScore

27.6

Impact factor

16.9

ISSN

14337851 (Print)

15213773 (Electronic)

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