Current Protocols, volume 2, issue 1
Synthesis of α‐ D ‐Ribose 1‐Phosphate and 2‐Deoxy‐α‐ D ‐Ribose 1‐Phosphate Via Enzymatic Phosphorolysis of 7‐Methylguanosine and 7‐Methyldeoxyguanosine
Publication type: Journal Article
Publication date: 2022-01-20
Journal:
Current Protocols
Quartile SCImago
Q1
Quartile WOS
—
Impact factor: —
ISSN: 26911299, 26911299
DOI:
10.1002/cpz1.347
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Pharmacology, Toxicology and Pharmaceutics
General Neuroscience
Medical Laboratory Technology
Health Informatics
Abstract
A simple and efficient method for the preparation of α‐D‐ribose 1‐phosphate and 2‐deoxy‐α‐D‐ribose 1‐phosphate, key intermediates in nucleoside metabolism and important starting compounds for the enzymatic synthesis of various modified nucleosides, has been proposed. It consists in near‐irreversible enzymatic phosphorolysis of readily prepared hydroiodide salts of 7‐methylguanosine and 7‐methyl‐2′‐deoxyguanosine, respectively, in the presence of purine nucleoside phosphorylase. α‐D‐Ribose 1‐phosphate and 2‐deoxy‐α‐D‐ribose 1‐phosphate are obtained in near quantitative yields (by HPLC analysis) and 74%‐94% yields after their isolation and purification. © 2022 Wiley Periodicals LLC.
Basic Protocol 1: Preparation of α‐D‐ribose 1‐phosphate barium salt (4a)
Alternate Protocol 1: Preparation of 2‐deoxy‐α‐D‐ribose 1‐phosphate barium salt (4b)
Basic Protocol 2: Preparation of α‐D‐ribose 1‐phosphate bis(cyclohexylammonium) salt (5a)
Alternate Protocol 2: Preparation of 2‐deoxy‐α‐D‐ribose 1‐phosphate bis(cyclohexylammonium) salt (5b)
Top-30
Citations by journals
|
1
|
|
|
International Journal of Molecular Sciences
1 publication, 50%
|
|
|
ChemCatChem
1 publication, 50%
|
|
|
1
|
Citations by publishers
|
1
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 50%
|
|
|
Wiley
1 publication, 50%
|
|
|
1
|
- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2022],"ids":[0],"codes":[0],"imageUrls":[""],"datasets":[{"label":"Citations number","data":[2],"backgroundColor":["#3B82F6"],"percentage":["100"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["International Journal of Molecular Sciences","ChemCatChem"],"ids":[14627,10149],"codes":[0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp"],"datasets":[{"label":"","data":[1,1],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":[50,50],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Wiley"],"ids":[202,11],"codes":[0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp"],"datasets":[{"label":"","data":[1,1],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":[50,50],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartiles":{"type":"bar","data":{"show":true,"labels":[2022],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0],"percentage":["0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0],"percentage":["0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[0],"percentage":["0"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[2],"percentage":["100"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[0],"percentage":["0"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by SCImago per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartilesWs":{"type":"bar","data":{"show":true,"labels":[2022],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0],"percentage":["0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0],"percentage":["0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[1],"percentage":["50"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[1],"percentage":["50"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[0],"percentage":["0"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by WoS per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Varizhuk I. V. et al. Synthesis of α‐ D ‐Ribose 1‐Phosphate and 2‐Deoxy‐α‐ D ‐Ribose 1‐Phosphate Via Enzymatic Phosphorolysis of 7‐Methylguanosine and 7‐Methyldeoxyguanosine // Current Protocols. 2022. Vol. 2. No. 1.
GOST all authors (up to 50)
Copy
Varizhuk I. V., Oslovsky V. E., Solyev P. N., Drenichev M. S., Mikhailov S. N. Synthesis of α‐ D ‐Ribose 1‐Phosphate and 2‐Deoxy‐α‐ D ‐Ribose 1‐Phosphate Via Enzymatic Phosphorolysis of 7‐Methylguanosine and 7‐Methyldeoxyguanosine // Current Protocols. 2022. Vol. 2. No. 1.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1002/cpz1.347
UR - https://doi.org/10.1002/cpz1.347
TI - Synthesis of α‐ D ‐Ribose 1‐Phosphate and 2‐Deoxy‐α‐ D ‐Ribose 1‐Phosphate Via Enzymatic Phosphorolysis of 7‐Methylguanosine and 7‐Methyldeoxyguanosine
T2 - Current Protocols
AU - Varizhuk, Irina V
AU - Oslovsky, Vladimir E
AU - Solyev, Pavel N
AU - Drenichev, Mikhail S
AU - Mikhailov, Sergey N.
PY - 2022
DA - 2022/01/20 00:00:00
PB - Wiley
IS - 1
VL - 2
SN - 2691-1299
SN - 2691-1299
ER -
Cite this
BibTex
Copy
@article{2022_Varizhuk,
author = {Irina V Varizhuk and Vladimir E Oslovsky and Pavel N Solyev and Mikhail S Drenichev and Sergey N. Mikhailov},
title = {Synthesis of α‐ D ‐Ribose 1‐Phosphate and 2‐Deoxy‐α‐ D ‐Ribose 1‐Phosphate Via Enzymatic Phosphorolysis of 7‐Methylguanosine and 7‐Methyldeoxyguanosine},
journal = {Current Protocols},
year = {2022},
volume = {2},
publisher = {Wiley},
month = {jan},
url = {https://doi.org/10.1002/cpz1.347},
number = {1},
doi = {10.1002/cpz1.347}
}