Optimization of ultrasonic-assisted extraction of anthocyanins from Prunus yedoensis fruit and evaluation of antioxidant and anti-inflammatory activity

Prunus yedoensis Matsumura is an important medicinal species in traditional Korean medicine, commonly employed for addressing a range of health issues. The fruit of Prunus yedoensis is abundant in anthocyanins, which possess strong antioxidant activity and effectively neutralize free radicals. In the present investigation, to optimize the extraction parameters for anthocyanins obtained from the fruit of P. yedoensis, response surface methodology was employed. Extraction time, ethanol concentration, and extraction temperature were carefully examined as key parameters to determine their influence on the yield and efficiency of anthocyanin extraction. Ultrasonic-assisted extraction extracted in this way showed higher antioxidant activity than conventional extract. In addition, the anti-inflammatory activity of UAE was confirmed, and changes in nitric oxide, inducible nitric oxide synthase, tumor necrosis factor alpha, and Interleukin-1 beta were observed. In conclusion, the extraction method of anthocyanins from P. yedoensis was optimized, and UAE extracted in this way had high antioxidant and anti-inflammatory activities.