Plant Cell, Tissue and Organ Culture, volume 145, issue 3, pages 591-600
Stability of cryopreserved Polyscias filicifolia suspension cell culture during cultivation in laboratory and industrial bioreactors
Titova M V
1
,
Popova Elena
1
,
Shumilo N A
1
,
Kulichenko I E
1
,
Chernyak N D
1
,
Ivanov I.M.
1
,
Klushin A G
1
,
Nosov A. M.
1, 2
Publication type: Journal Article
Publication date: 2021-02-20
Journal:
Plant Cell, Tissue and Organ Culture
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 3
ISSN: 01676857, 15735044
Horticulture
Abstract
Cell suspension of a medicinal plant Polyscias filicifolia recovered after 5 years of cryopreservation in liquid nitrogen (− 196 °C) was cultured in 250 ml flasks and laboratory (20-l), pilot (75-l) and industrial (630-l) bioreactors operated under batch and semi-continuous regimes. Growth parameters of the recovered cell culture were compared to those recorded before cryopreservation and after 5 years of culture maintenance in the active collection with 2 weeks subculture intervals. The results revealed that cell viability, maximum accumulation of fresh and dry weights, specific growth rate and productivity on dry biomass in flasks and bioreactors of different types and volumes were not affected by cryopreservation. In 20-l bioreactors, longer lag phase was observed for cell culture maintained for 5 years by periodic subcultures compared to the initial and cryopreserved cell cultures, however, all differences in growth dynamics were mitigated during cultivation in 630-l bioreactors. This study demonstrates that cryopreservation is a reliable and effective method for conservation of P. filicifolia cell culture for biotechnological applications. Cell suspension of a medicinal plant Polyscias filicifolia recovered after 5 years of cryopreservation fully retained its original growth and productivity when cultured in bioreactors from 20 to 630 l.
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Citations by publishers
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5
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Multidisciplinary Digital Publishing Institute (MDPI)
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5 publications, 83.33%
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Elsevier
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1 publication, 16.67%
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1
2
3
4
5
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Titova M. V. et al. Stability of cryopreserved Polyscias filicifolia suspension cell culture during cultivation in laboratory and industrial bioreactors // Plant Cell, Tissue and Organ Culture. 2021. Vol. 145. No. 3. pp. 591-600.
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Titova M. V., Popova E., Shumilo N. A., Kulichenko I. E., Chernyak N. D., Ivanov I., Klushin A. G., Nosov A. M. Stability of cryopreserved Polyscias filicifolia suspension cell culture during cultivation in laboratory and industrial bioreactors // Plant Cell, Tissue and Organ Culture. 2021. Vol. 145. No. 3. pp. 591-600.
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TY - JOUR
DO - 10.1007/s11240-021-02030-5
UR - https://doi.org/10.1007%2Fs11240-021-02030-5
TI - Stability of cryopreserved Polyscias filicifolia suspension cell culture during cultivation in laboratory and industrial bioreactors
T2 - Plant Cell, Tissue and Organ Culture
AU - Titova, M V
AU - Popova, Elena
AU - Shumilo, N A
AU - Kulichenko, I E
AU - Chernyak, N D
AU - Ivanov, I.M.
AU - Klushin, A G
AU - Nosov, A. M.
PY - 2021
DA - 2021/02/20 00:00:00
PB - Springer Nature
SP - 591-600
IS - 3
VL - 145
SN - 0167-6857
SN - 1573-5044
ER -
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@article{2021_Titova,
author = {M V Titova and Elena Popova and N A Shumilo and I E Kulichenko and N D Chernyak and I.M. Ivanov and A G Klushin and A. M. Nosov},
title = {Stability of cryopreserved Polyscias filicifolia suspension cell culture during cultivation in laboratory and industrial bioreactors},
journal = {Plant Cell, Tissue and Organ Culture},
year = {2021},
volume = {145},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1007%2Fs11240-021-02030-5},
number = {3},
pages = {591--600},
doi = {10.1007/s11240-021-02030-5}
}
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Titova, M. V., et al. “Stability of cryopreserved Polyscias filicifolia suspension cell culture during cultivation in laboratory and industrial bioreactors.” Plant Cell, Tissue and Organ Culture, vol. 145, no. 3, Feb. 2021, pp. 591-600. https://doi.org/10.1007%2Fs11240-021-02030-5.
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