Biotechnology Letters

, volume 9 , issue 10 , pages 725-730

Protein stabilization via hydrophilization: Stabilization of α-chymotrypsin by reductive alkylation with glyoxylic acid

N.S. Melik-Nubarov ¹

V.V Mozhaev ¹

S Šikšnis ²

Karel Martinek ³

Hide authors affiliations Show authors affiliations: 3 affiliations

Chemistry Department, Moscow State University, Moscow, (USSR) | |

All-Union Research Institute of Applied Enzymology, Vilnius, Lithuanian SSR (USSR) | |

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague, (Czechoslovakia) | |

Publication type: Journal Article

Publication date: 1987-10-01

Springer Nature

Biotechnology Letters

scimago Q2

wos Q3

SJR: 0.528

CiteScore: 5.4

Impact factor: 2.1

ISSN: 01415492, 15736776

DOI: 10.1007/BF01024608

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General Medicine

Applied Microbiology and Biotechnology

Biotechnology

Bioengineering

Abstract

Reductive alkylation of amino groups in α-chymotrypsin by glyoxylic acid in the presence of sodium cyanoborohydride results in essential stabilization of the enzyme against irreversible thermoinactivation. At 60°C, the stabilizing effect is higher than one thousand, and it increases even more with rising the temperature.

Found

2 citations

Melik-Nubarov Nikolay

🥼

DSc in Chemistry

95 publications, 2 642 citations, 3 reviews

h-index: 25

Lomonosov Moscow State University

Research interests

Chemically induced photodynamic teramia

Drug delivery

High molecular weight compounds

Photodynamic Therapy (PDT)

Synthesis of amphiphilic biodegradable copolymers

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GOST |

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GOST Copy

Melik-Nubarov N. et al. Protein stabilization via hydrophilization: Stabilization of α-chymotrypsin by reductive alkylation with glyoxylic acid // Biotechnology Letters. 1987. Vol. 9. No. 10. pp. 725-730.

GOST all authors (up to 50) Copy

Melik-Nubarov N., Mozhaev V., Šikšnis S., Martinek K. Protein stabilization via hydrophilization: Stabilization of α-chymotrypsin by reductive alkylation with glyoxylic acid // Biotechnology Letters. 1987. Vol. 9. No. 10. pp. 725-730.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1007/BF01024608

UR - http://link.springer.com/10.1007/BF01024608

TI - Protein stabilization via hydrophilization: Stabilization of α-chymotrypsin by reductive alkylation with glyoxylic acid

T2 - Biotechnology Letters

AU - Melik-Nubarov, N.S.

AU - Mozhaev, V.V

AU - Šikšnis, S

AU - Martinek, Karel

PY - 1987

DA - 1987/10/01

PB - Springer Nature

SP - 725-730

IS - 10

VL - 9

SN - 0141-5492

SN - 1573-6776

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{1987_Melik-Nubarov,

author = {N.S. Melik-Nubarov and V.V Mozhaev and S Šikšnis and Karel Martinek},

title = {Protein stabilization via hydrophilization: Stabilization of α-chymotrypsin by reductive alkylation with glyoxylic acid},

journal = {Biotechnology Letters},

year = {1987},

volume = {9},

publisher = {Springer Nature},

month = {oct},

url = {http://link.springer.com/10.1007/BF01024608},

number = {10},

pages = {725--730},

doi = {10.1007/BF01024608}

}

MLA

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MLA Copy

Melik-Nubarov, N.S., et al. “Protein stabilization via hydrophilization: Stabilization of α-chymotrypsin by reductive alkylation with glyoxylic acid.” Biotechnology Letters, vol. 9, no. 10, Oct. 1987, pp. 725-730. http://link.springer.com/10.1007/BF01024608.