Cellular and Molecular Life Sciences, volume 78, issue 23, pages 7777-7794
Snake venom phospholipase A2s exhibit strong virucidal activity against SARS-CoV-2 and inhibit the viral spike glycoprotein interaction with ACE2
Siniavin Andrei
1, 2
,
Nikiforova Maria
2
,
Grinkina Svetlana D
2
,
Гущин В. А.
2
,
Mozhaeva Vera A
1, 4
,
Starkov Vladislav G.
1
,
Osipov Alexey V.
1
,
Lummis Sarah C.R.
5
,
Tsetlin V. I.
1
,
Publication type: Journal Article
Publication date: 2021-10-29
Journal:
Cellular and Molecular Life Sciences
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 8
ISSN: 1420682X, 14209071
PubMed ID:
34714362
Molecular Biology
Pharmacology
Cell Biology
Molecular Medicine
Cellular and Molecular Neuroscience
Abstract
The COVID-19 pandemic caused by SARS-CoV-2 requires new treatments both to alleviate the symptoms and to prevent the spread of this disease. Previous studies demonstrated good antiviral and virucidal activity of phospholipase A2s (PLA2s) from snake venoms against viruses from different families but there was no data for coronaviruses. Here we show that PLA2s from snake venoms protect Vero E6 cells against SARS-CoV-2 cytopathic effects. PLA2s showed low cytotoxicity to Vero E6 cells with some activity at micromolar concentrations, but strong antiviral activity at nanomolar concentrations. Dimeric PLA2 from the viper Vipera nikolskii and its subunits manifested especially potent virucidal effects, which were related to their phospholipolytic activity, and inhibited cell–cell fusion mediated by the SARS-CoV-2 spike glycoprotein. Moreover, PLA2s interfered with binding both of an antibody against ACE2 and of the receptor-binding domain of the glycoprotein S to 293T/ACE2 cells. This is the first demonstration of a detrimental effect of PLA2s on β-coronaviruses. Thus, snake PLA2s are promising for the development of antiviral drugs that target the viral envelope, and could also prove to be useful tools to study the interaction of viruses with host cells. Supplementary Information The online version contains supplementary material available at 10.1007/s00018-021-03985-6.
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1 publication, 3.7%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Siniavin A. et al. Snake venom phospholipase A2s exhibit strong virucidal activity against SARS-CoV-2 and inhibit the viral spike glycoprotein interaction with ACE2 // Cellular and Molecular Life Sciences. 2021. Vol. 78. No. 23. pp. 7777-7794.
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Siniavin A., Streltsova M. A., Nikiforova M., Kudryavtsev D. S., Grinkina S. D., Гущин В. А., Mozhaeva V. A., Starkov V. G., Osipov A. V., Lummis S. C., Tsetlin V. I., Utkin Y. N. Snake venom phospholipase A2s exhibit strong virucidal activity against SARS-CoV-2 and inhibit the viral spike glycoprotein interaction with ACE2 // Cellular and Molecular Life Sciences. 2021. Vol. 78. No. 23. pp. 7777-7794.
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TY - JOUR
DO - 10.1007/s00018-021-03985-6
UR - https://doi.org/10.1007%2Fs00018-021-03985-6
TI - Snake venom phospholipase A2s exhibit strong virucidal activity against SARS-CoV-2 and inhibit the viral spike glycoprotein interaction with ACE2
T2 - Cellular and Molecular Life Sciences
AU - Siniavin, Andrei
AU - Streltsova, Maria A
AU - Kudryavtsev, Denis S.
AU - Grinkina, Svetlana D
AU - Гущин, В. А.
AU - Mozhaeva, Vera A
AU - Starkov, Vladislav G.
AU - Lummis, Sarah C.R.
AU - Tsetlin, V. I.
AU - Utkin, Yu. N.
AU - Osipov, Alexey V.
AU - Nikiforova, Maria
PY - 2021
DA - 2021/10/29 00:00:00
PB - Springer Nature
SP - 7777-7794
IS - 23
VL - 78
PMID - 34714362
SN - 1420-682X
SN - 1420-9071
ER -
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@article{2021_Siniavin,
author = {Andrei Siniavin and Maria A Streltsova and Denis S. Kudryavtsev and Svetlana D Grinkina and В. А. Гущин and Vera A Mozhaeva and Vladislav G. Starkov and Sarah C.R. Lummis and V. I. Tsetlin and Yu. N. Utkin and Alexey V. Osipov and Maria Nikiforova},
title = {Snake venom phospholipase A2s exhibit strong virucidal activity against SARS-CoV-2 and inhibit the viral spike glycoprotein interaction with ACE2},
journal = {Cellular and Molecular Life Sciences},
year = {2021},
volume = {78},
publisher = {Springer Nature},
month = {oct},
url = {https://doi.org/10.1007%2Fs00018-021-03985-6},
number = {23},
pages = {7777--7794},
doi = {10.1007/s00018-021-03985-6}
}
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Siniavin, Andrei, et al. “Snake venom phospholipase A2s exhibit strong virucidal activity against SARS-CoV-2 and inhibit the viral spike glycoprotein interaction with ACE2.” Cellular and Molecular Life Sciences, vol. 78, no. 23, Oct. 2021, pp. 7777-7794. https://doi.org/10.1007%2Fs00018-021-03985-6.