A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria
Тип публикации: Journal Article
Дата публикации: 2011-09-23
SCImago Q2
WOS Q2
БС1
SJR: 0.717
CiteScore: 8
Impact factor: 4.1
ISSN: 16182642, 16182650
PubMed ID:
21947437
Biochemistry
Analytical Chemistry
Краткое описание
We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria. The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3 × 103 to 4.3 × 105 cells/mL. The method has potential for routine application, e.g. for drinking water control.
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ГОСТ
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Knauer M. et al. A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria // Analytical and Bioanalytical Chemistry. 2011. Vol. 402. No. 8. pp. 2663-2667.
ГОСТ со всеми авторами (до 50)
Скопировать
Knauer M., Ivleva N. P., Niessner R., Haisch C. A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria // Analytical and Bioanalytical Chemistry. 2011. Vol. 402. No. 8. pp. 2663-2667.
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RIS
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TY - JOUR
DO - 10.1007/s00216-011-5398-0
UR - https://doi.org/10.1007/s00216-011-5398-0
TI - A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria
T2 - Analytical and Bioanalytical Chemistry
AU - Knauer, Maria
AU - Ivleva, Natalia P.
AU - Niessner, Reinhard
AU - Haisch, Christoph
PY - 2011
DA - 2011/09/23
PB - Springer Nature
SP - 2663-2667
IS - 8
VL - 402
PMID - 21947437
SN - 1618-2642
SN - 1618-2650
ER -
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BibTex (до 50 авторов)
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@article{2011_Knauer,
author = {Maria Knauer and Natalia P. Ivleva and Reinhard Niessner and Christoph Haisch},
title = {A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria},
journal = {Analytical and Bioanalytical Chemistry},
year = {2011},
volume = {402},
publisher = {Springer Nature},
month = {sep},
url = {https://doi.org/10.1007/s00216-011-5398-0},
number = {8},
pages = {2663--2667},
doi = {10.1007/s00216-011-5398-0}
}
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MLA
Скопировать
Knauer, Maria, et al. “A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria.” Analytical and Bioanalytical Chemistry, vol. 402, no. 8, Sep. 2011, pp. 2663-2667. https://doi.org/10.1007/s00216-011-5398-0.
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