Histochemistry and Cell Biology, volume 145, issue 4, pages 419-432
Mechanisms of nuclear lamina growth in interphase
Kurchashova Svetlana Yu.
1
,
Pozharskaia Vasilisa A
2
,
Cherepanynets Varvara D
1
,
Strelkova Olga S
1
,
Hozak Pavel
3
,
Kireev Igor I
1, 4
3
Institute of Molecular Genetics of the ASCR, Prague 4, Czech Republic
|
Publication type: Journal Article
Publication date: 2016-02-16
Journal:
Histochemistry and Cell Biology
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 2.3
ISSN: 09486143, 1432119X
Molecular Biology
Cell Biology
Histology
Medical Laboratory Technology
Abstract
Abstract
The nuclear lamina represents a multifunctional platform involved in such diverse yet interconnected processes as spatial organization of the genome, maintenance of mechanical stability of the nucleus, regulation of transcription and replication. Most of lamina activities are exerted through tethering of lamina-associated chromatin domains (LADs) to the nuclear periphery. Yet, the lamina is a dynamic structure demonstrating considerable expansion during the cell cycle to accommodate increased number of LADs formed during DNA replication. We analyzed dynamics of nuclear growth during interphase and changes in lamina structure as a function of cell cycle progression. The nuclear lamina demonstrates steady growth from G1 till G2, while quantitative analysis of lamina meshwork by super-resolution microscopy revealed that microdomain organization of the lamina is maintained, with lamin A and lamin B microdomain periodicity and interdomain gap sizes unchanged. FRAP analysis, in contrast, demonstrated differences in lamin A and B1 exchange rates; the latter showing higher recovery rate in S-phase cells. In order to further analyze the mechanism of lamina growth in interphase, we generated a lamina-free nuclear envelope in living interphase cells by reversible hypotonic shock. The nuclear envelope in nuclear buds formed after such a treatment initially lacked lamins, and analysis of lamina formation revealed striking difference in lamin A and B1 assembly: lamin A reassembled within 30 min post-treatment, whereas lamin B1 did not incorporate into the newly formed lamina at all. We suggest that in somatic cells lamin B1 meshwork growth is coordinated with replication of LADs, and lamin A meshwork assembly seems to be chromatin-independent process.
Citations by journals
|
1
|
|
|
Frontiers in Genetics
|
Frontiers in Genetics
1 publication, 14.29%
|
|
Cell and Tissue Biology
|
Cell and Tissue Biology
1 publication, 14.29%
|
|
Biochemistry. Biokhimiia
|
Biochemistry. Biokhimiia
1 publication, 14.29%
|
|
Биохимия
|
Биохимия
1 publication, 14.29%
|
|
Cells
|
Cells
1 publication, 14.29%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
1 publication, 14.29%
|
|
Molecular Biology of the Cell
|
Molecular Biology of the Cell
1 publication, 14.29%
|
|
1
|
Citations by publishers
|
1
2
|
|
|
Pleiades Publishing
|
Pleiades Publishing
2 publications, 28.57%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 28.57%
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
1 publication, 14.29%
|
|
Akademizdatcenter Nauka
|
Akademizdatcenter Nauka
1 publication, 14.29%
|
|
American Society for Cell Biology (ASCB)
|
American Society for Cell Biology (ASCB)
1 publication, 14.29%
|
|
1
2
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2017,2018,2019,2020,2021,2022,2023],"ids":[0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0],"imageUrls":["","","","","","",""],"datasets":[{"label":"Citations number","data":[1,0,2,0,3,0,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["14.29",0,"28.57",0,"42.86",0,"14.29"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Frontiers in Genetics","Cell and Tissue Biology","Biochemistry. Biokhimiia","\u0411\u0438\u043e\u0445\u0438\u043c\u0438\u044f","Cells","International Journal of Molecular Sciences","Molecular Biology of the Cell"],"ids":[13242,16847,25428,27074,25293,14627,18406],"codes":[0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/QvUjpflupmitGM5fuTKfMKbjgX15XAHq011OUdeD_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/1pFWD0EzQnc5egizpglbyNDhAbHCAVj1O7hBchfP_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[14.29,14.29,14.29,14.29,14.29,14.29,14.29],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Pleiades Publishing","Multidisciplinary Digital Publishing Institute (MDPI)","Frontiers Media S.A.","Akademizdatcenter Nauka","American Society for Cell Biology (ASCB)"],"ids":[101,202,208,6986,4151],"codes":[0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/QvUjpflupmitGM5fuTKfMKbjgX15XAHq011OUdeD_medium.webp","\/storage\/images\/resized\/1pFWD0EzQnc5egizpglbyNDhAbHCAVj1O7hBchfP_medium.webp"],"datasets":[{"label":"","data":[2,2,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[28.57,28.57,14.29,14.29,14.29],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Zhironkina O. A. et al. Mechanisms of nuclear lamina growth in interphase // Histochemistry and Cell Biology. 2016. Vol. 145. No. 4. pp. 419-432.
GOST all authors (up to 50)
Copy
Zhironkina O. A., Kurchashova S., Pozharskaia V. A., Cherepanynets V. D., Strelkova O. S., Hozak P., Kireev I. I. Mechanisms of nuclear lamina growth in interphase // Histochemistry and Cell Biology. 2016. Vol. 145. No. 4. pp. 419-432.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1007/s00418-016-1419-6
UR - https://doi.org/10.1007%2Fs00418-016-1419-6
TI - Mechanisms of nuclear lamina growth in interphase
T2 - Histochemistry and Cell Biology
AU - Pozharskaia, Vasilisa A
AU - Cherepanynets, Varvara D
AU - Hozak, Pavel
AU - Zhironkina, Oxana A
AU - Kurchashova, Svetlana Yu.
AU - Strelkova, Olga S
AU - Kireev, Igor I
PY - 2016
DA - 2016/02/16 00:00:00
PB - Springer Nature
SP - 419-432
IS - 4
VL - 145
SN - 0948-6143
SN - 1432-119X
ER -
Cite this
BibTex
Copy
@article{2016_Zhironkina,
author = {Vasilisa A Pozharskaia and Varvara D Cherepanynets and Pavel Hozak and Oxana A Zhironkina and Svetlana Yu. Kurchashova and Olga S Strelkova and Igor I Kireev},
title = {Mechanisms of nuclear lamina growth in interphase},
journal = {Histochemistry and Cell Biology},
year = {2016},
volume = {145},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1007%2Fs00418-016-1419-6},
number = {4},
pages = {419--432},
doi = {10.1007/s00418-016-1419-6}
}
Cite this
MLA
Copy
Zhironkina, Oxana A., et al. “Mechanisms of nuclear lamina growth in interphase.” Histochemistry and Cell Biology, vol. 145, no. 4, Feb. 2016, pp. 419-432. https://doi.org/10.1007%2Fs00418-016-1419-6.