Protoplasma, volume 254, issue 3, pages 1323-1340
Versatility of the green microalga cell vacuole function as revealed by analytical transmission electron microscopy
Shebanova Anastasia
1
,
Ismagulova Tatiana
1
,
Solovchenko Alexei E.
1, 2, 3
,
Baulina Olga
1
,
Lobakova Elena
1
,
Ivanova Alexandra
4, 5
,
Moiseenko Andrey
1
,
Shaitan Konstantin
1
,
Nedbal Ladislav
7
,
Gorelova Olga
1
7
Institute of Bio- and Geosciences / Plant Sciences (IBG-2), Forschungszentrum Jülich, Jülich, Germany
|
Publication type: Journal Article
Publication date: 2016-09-27
Journal:
Protoplasma
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 2.9
ISSN: 0033183X, 16156102, 09348727
General Medicine
Cell Biology
Plant Science
Abstract
Vacuole is a multifunctional compartment central to a large number of functions (storage, catabolism, maintenance of the cell homeostasis) in oxygenic phototrophs including microalgae. Still, microalgal cell vacuole is much less studied than that of higher plants although knowledge of the vacuolar structure and function is essential for understanding physiology of nutrition and stress tolerance of microalgae. Here, we combined the advanced analytical and conventional transmission electron microscopy methods to obtain semi-quantitative, spatially resolved at the subcellular level information on elemental composition of the cell vacuoles in several free-living and symbiotic chlorophytes. We obtained a detailed record of the changes in cell and vacuolar ultrastructure in response to environmental stimuli under diverse conditions. We suggested that the vacuolar inclusions could be divided into responsible for storage of phosphorus (mainly in form of polyphosphate) and those accommodating non-protein nitrogen (presumably polyamine) reserves, respectively.The ultrastructural findings, together with the data on elemental composition of different cell compartments, allowed us to speculate on the role of the vacuolar membrane in the biosynthesis and sequestration of polyphosphate. We also describe the ultrastructural evidence of possible involvement of the tonoplast in the membrane lipid turnover and exchange of energy and metabolites between chloroplasts and mitochondria. These processes might play a significant role in acclimation in different stresses including nitrogen starvation and extremely high level of CO2 and might also be of importance for microalgal biotechnology. Advantages and limitations of application of analytical electron microscopy to biosamples such as microalgal cells are discussed.
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1 publication, 2.13%
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1 publication, 2.13%
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Kalvis, 1, 2.13%
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1 publication, 2.13%
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1 publication, 2.13%
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2
4
6
8
10
12
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Shebanova A. et al. Versatility of the green microalga cell vacuole function as revealed by analytical transmission electron microscopy // Protoplasma. 2016. Vol. 254. No. 3. pp. 1323-1340.
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Shebanova A., Ismagulova T., Solovchenko A. E., Baulina O., Lobakova E., Ivanova A., Moiseenko A., Shaitan K., Polshakov V., Nedbal L., Gorelova O. Versatility of the green microalga cell vacuole function as revealed by analytical transmission electron microscopy // Protoplasma. 2016. Vol. 254. No. 3. pp. 1323-1340.
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TY - JOUR
DO - 10.1007/s00709-016-1024-5
UR - https://doi.org/10.1007%2Fs00709-016-1024-5
TI - Versatility of the green microalga cell vacuole function as revealed by analytical transmission electron microscopy
T2 - Protoplasma
AU - Shebanova, Anastasia
AU - Ismagulova, Tatiana
AU - Solovchenko, Alexei E.
AU - Baulina, Olga
AU - Lobakova, Elena
AU - Moiseenko, Andrey
AU - Shaitan, Konstantin
AU - Polshakov, Vladimir
AU - Nedbal, Ladislav
AU - Gorelova, Olga
AU - Ivanova, Alexandra
PY - 2016
DA - 2016/09/27 00:00:00
PB - Springer Nature
SP - 1323-1340
IS - 3
VL - 254
SN - 0033-183X
SN - 1615-6102
SN - 0934-8727
ER -
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@article{2016_Shebanova,
author = {Anastasia Shebanova and Tatiana Ismagulova and Alexei E. Solovchenko and Olga Baulina and Elena Lobakova and Andrey Moiseenko and Konstantin Shaitan and Vladimir Polshakov and Ladislav Nedbal and Olga Gorelova and Alexandra Ivanova},
title = {Versatility of the green microalga cell vacuole function as revealed by analytical transmission electron microscopy},
journal = {Protoplasma},
year = {2016},
volume = {254},
publisher = {Springer Nature},
month = {sep},
url = {https://doi.org/10.1007%2Fs00709-016-1024-5},
number = {3},
pages = {1323--1340},
doi = {10.1007/s00709-016-1024-5}
}
Cite this
MLA
Copy
Shebanova, Anastasia, et al. “Versatility of the green microalga cell vacuole function as revealed by analytical transmission electron microscopy.” Protoplasma, vol. 254, no. 3, Sep. 2016, pp. 1323-1340. https://doi.org/10.1007%2Fs00709-016-1024-5.