Molecular Imaging and Biology, volume 20, issue 3, pages 368-377
Cytotoxicity Test Based on Human Cells Labeled with Fluorescent Proteins: Fluorimetry, Photography, and Scanning for High-Throughput Assay
Kalinina Marina A
1, 2
,
Skvortsov Dmitry
2
,
Rubtsova Maria P
1, 2
,
Komarova Ekaterina S.
1, 2
,
Dontsova Olga A.
1, 2
Publication type: Journal Article
Publication date: 2017-12-21
Journal:
Molecular Imaging and Biology
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3.1
ISSN: 15361632, 18602002
Cancer Research
Oncology
Radiology Nuclear Medicine and imaging
Abstract
PurposeHigh- and medium-throughput assays are now routine methods for drug screening and toxicology investigations on mammalian cells. However, a simple and cost-effective analysis of cytotoxicity that can be carried out with commonly used laboratory equipment is still required.ProceduresThe developed cytotoxicity assays are based on human cell lines stably expressing eGFP, tdTomato, mCherry, or Katushka2S fluorescent proteins. Red fluorescent proteins exhibit a higher signal-to-noise ratio, due to less interference by medium autofluorescence, in comparison to green fluorescent protein. Measurements have been performed on a fluorescence scanner, a plate fluorimeter, and a camera photodocumentation system.ResultsFor a 96-well plate assay, the sensitivity per well and the measurement duration were 250 cells and 15 min for the scanner, 500 cells and 2 min for the plate fluorimeter, and 1000 cells and less than 1 min for the camera detection. These sensitivities are similar to commonly used MTT (tetrazolium dye) assays. The used scanner and the camera had not been previously applied for cytotoxicity evaluation. An image processing scheme for the high-resolution scanner is proposed that significantly diminishes the number of control wells, even for a library containing fluorescent substances. The suggested cytotoxicity assay has been verified by measurements of the cytotoxicity of several well-known cytotoxic drugs and further applied to test a set of novel bacteriotoxic compounds in a medium-throughput format.ConclusionThe fluorescent signal of living cells is detected without disturbing them and adding any reagents, thus allowing to investigate time-dependent cytotoxicity effects on the same sample of cells. A fast, simple and cost-effective assay is suggested for cytotoxicity evaluation based on mammalian cells expressing fluorescent proteins and commonly used laboratory equipment.
Citations by journals
|
1
2
3
4
|
|
|
Russian Chemical Bulletin
|
Russian Chemical Bulletin
4 publications, 44.44%
|
|
Journal of Organic Chemistry
|
Journal of Organic Chemistry
1 publication, 11.11%
|
|
Archiv der Pharmazie
|
Archiv der Pharmazie
1 publication, 11.11%
|
|
Journal of Medicinal Chemistry
|
Journal of Medicinal Chemistry
1 publication, 11.11%
|
|
Frontiers in Pharmacology
|
Frontiers in Pharmacology
1 publication, 11.11%
|
|
Mendeleev Communications
|
Mendeleev Communications
1 publication, 11.11%
|
|
1
2
3
4
|
Citations by publishers
|
1
2
3
4
|
|
|
Springer Nature
|
Springer Nature
4 publications, 44.44%
|
|
American Chemical Society (ACS)
|
American Chemical Society (ACS)
2 publications, 22.22%
|
|
Wiley
|
Wiley
1 publication, 11.11%
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
1 publication, 11.11%
|
|
Elsevier
|
Elsevier
1 publication, 11.11%
|
|
1
2
3
4
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2019,2020,2021,2022,2023],"ids":[0,0,0,0,0],"codes":[0,0,0,0,0],"imageUrls":["","","","",""],"datasets":[{"label":"Citations number","data":[1,4,2,0,2],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["11.11","44.44","22.22",0,"22.22"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Russian Chemical Bulletin","Journal of Organic Chemistry","Archiv der Pharmazie","Journal of Medicinal Chemistry","Frontiers in Pharmacology","Mendeleev Communications"],"ids":[10918,8697,15226,3673,9788,5294],"codes":[0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[4,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[44.44,11.11,11.11,11.11,11.11,11.11],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Springer Nature","American Chemical Society (ACS)","Wiley","Frontiers Media S.A.","Elsevier"],"ids":[8,40,11,208,17],"codes":[0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[4,2,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[44.44,22.22,11.11,11.11,11.11],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Kalinina M. A. et al. Cytotoxicity Test Based on Human Cells Labeled with Fluorescent Proteins: Fluorimetry, Photography, and Scanning for High-Throughput Assay // Molecular Imaging and Biology. 2017. Vol. 20. No. 3. pp. 368-377.
GOST all authors (up to 50)
Copy
Kalinina M. A., Skvortsov D., Rubtsova M. P., Komarova E. S., Dontsova O. A. Cytotoxicity Test Based on Human Cells Labeled with Fluorescent Proteins: Fluorimetry, Photography, and Scanning for High-Throughput Assay // Molecular Imaging and Biology. 2017. Vol. 20. No. 3. pp. 368-377.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1007/s11307-017-1152-0
UR - https://doi.org/10.1007%2Fs11307-017-1152-0
TI - Cytotoxicity Test Based on Human Cells Labeled with Fluorescent Proteins: Fluorimetry, Photography, and Scanning for High-Throughput Assay
T2 - Molecular Imaging and Biology
AU - Kalinina, Marina A
AU - Skvortsov, Dmitry
AU - Rubtsova, Maria P
AU - Komarova, Ekaterina S.
AU - Dontsova, Olga A.
PY - 2017
DA - 2017/12/21 00:00:00
PB - Springer Nature
SP - 368-377
IS - 3
VL - 20
SN - 1536-1632
SN - 1860-2002
ER -
Cite this
BibTex
Copy
@article{2017_Kalinina,
author = {Marina A Kalinina and Dmitry Skvortsov and Maria P Rubtsova and Ekaterina S. Komarova and Olga A. Dontsova},
title = {Cytotoxicity Test Based on Human Cells Labeled with Fluorescent Proteins: Fluorimetry, Photography, and Scanning for High-Throughput Assay},
journal = {Molecular Imaging and Biology},
year = {2017},
volume = {20},
publisher = {Springer Nature},
month = {dec},
url = {https://doi.org/10.1007%2Fs11307-017-1152-0},
number = {3},
pages = {368--377},
doi = {10.1007/s11307-017-1152-0}
}
Cite this
MLA
Copy
Kalinina, Marina A., et al. “Cytotoxicity Test Based on Human Cells Labeled with Fluorescent Proteins: Fluorimetry, Photography, and Scanning for High-Throughput Assay.” Molecular Imaging and Biology, vol. 20, no. 3, Dec. 2017, pp. 368-377. https://doi.org/10.1007%2Fs11307-017-1152-0.