Journal of the American Society for Mass Spectrometry, volume 28, issue 8, pages 1600-1611
An EThcD-Based Method for Discrimination of Leucine and Isoleucine Residues in Tryptic Peptides
Publication type: Journal Article
Publication date: 2017-04-26
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 3.2
ISSN: 10440305, 18791123
Spectroscopy
Structural Biology
Abstract
An EThcD-based approach for the reliable discrimination of isomeric leucine and isoleucine residues in peptide de novo sequencing procedure has been proposed. A multistage fragmentation of peptide ions was performed with Orbitrap Elite mass spectrometer in electrospray ionization mode. At the first stage, z-ions were produced by ETD or ETcaD fragmentation of doubly or triply charged peptide precursor ions. These primary ions were further fragmented by HCD with broad-band ion isolation, and the resulting w-ions showed different mass for leucine and isoleucine residues. The procedure did not require manual isolation of specific z-ions prior to HCD stage. Forty-three tryptic peptides (3 to 27 residues) obtained by trypsinolysis of human serum albumin (HSA) and gp188 protein were analyzed. To demonstrate a proper solution for radical site migration problem, three non-tryptic peptides were also analyzed. A total of 93 leucine and isoleucine residues were considered and 83 of them were correctly identified. The developed approach can be a reasonable substitution for additional Edman degradation procedure, which is still used in peptide sequencing for leucine and isoleucine discrimination. Graphical Abstract ᅟ.
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- We do not take into account publications that without a DOI.
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Zhokhov S. S. et al. An EThcD-Based Method for Discrimination of Leucine and Isoleucine Residues in Tryptic Peptides // Journal of the American Society for Mass Spectrometry. 2017. Vol. 28. No. 8. pp. 1600-1611.
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Zhokhov S. S., Kovalev S., Samgina T. Yu., Lebedev A. T. An EThcD-Based Method for Discrimination of Leucine and Isoleucine Residues in Tryptic Peptides // Journal of the American Society for Mass Spectrometry. 2017. Vol. 28. No. 8. pp. 1600-1611.
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TY - JOUR
DO - 10.1007/s13361-017-1674-3
UR - https://doi.org/10.1007%2Fs13361-017-1674-3
TI - An EThcD-Based Method for Discrimination of Leucine and Isoleucine Residues in Tryptic Peptides
T2 - Journal of the American Society for Mass Spectrometry
AU - Samgina, Tatiana Yu
AU - Zhokhov, Sergey S
AU - Kovalev, Sergey
AU - Lebedev, Albert T.
PY - 2017
DA - 2017/04/26 00:00:00
PB - American Chemical Society (ACS)
SP - 1600-1611
IS - 8
VL - 28
SN - 1044-0305
SN - 1879-1123
ER -
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@article{2017_Zhokhov,
author = {Tatiana Yu Samgina and Sergey S Zhokhov and Sergey Kovalev and Albert T. Lebedev},
title = {An EThcD-Based Method for Discrimination of Leucine and Isoleucine Residues in Tryptic Peptides},
journal = {Journal of the American Society for Mass Spectrometry},
year = {2017},
volume = {28},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1007%2Fs13361-017-1674-3},
number = {8},
pages = {1600--1611},
doi = {10.1007/s13361-017-1674-3}
}
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MLA
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Zhokhov, Sergey S., et al. “An EThcD-Based Method for Discrimination of Leucine and Isoleucine Residues in Tryptic Peptides.” Journal of the American Society for Mass Spectrometry, vol. 28, no. 8, Apr. 2017, pp. 1600-1611. https://doi.org/10.1007%2Fs13361-017-1674-3.
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