Isolation, identification and virulence of the local entomopathogenic nematode Heterorhabditis indica in the southern Philippines against the sugarcane white grub, Leucopholis irrorata (Chevrolat) (Coleoptera: Scarabaeidae)
Rolando Ramos
1
,
Cynthia Ann Dichusa
1, 2
,
Sitaram Aryal
3
,
Elorde Crispolon
4
,
Neil Pep Dave Sumaya
1
,
NANETTE HOPE N. SUMAYA
2, 5
1
Department of Plant Pathology, College of Agriculture, University of Southern Mindanao, Cotabato, Philippines
|
4
Department of Entomology, College of Agriculture, University of Southern Mindanao, Kabacan, Cotabato, Philippines
|
Publication type: Journal Article
Publication date: 2022-05-11
scimago Q2
wos Q3
SJR: 0.375
CiteScore: 2.9
Impact factor: 1.2
ISSN: 17427584, 17427592
Ecology, Evolution, Behavior and Systematics
Insect Science
Abstract
Entomopathogenic nematodes (EPNs) are used as biocontrol agents of diverse insect pests. EPN diversity study was conducted in the southern Philippines (Cotabato province), encompassing a total of 5 municipalities with 25 Barangays (villages) for the potential biocontrol of the sugarcane white grub, Leucopholis irrorata. Of the 25 soil samples collected, only 5 harbored nematodes by using the Galleria-baiting technique. Through preliminary assessment of morphological data and confirmation analysis of D2-D3 segments of the 28S rDNA, locally-isolated nematodes were identified as Heterorhabditis indica (1 isolate), Metarhabditis rainai (3 isolates) and Oscheius rugaonensis (1 isolate). Moreover, the virulence of the H. indica (KRR4) infective juveniles (IJs) isolated from sugarcane soils was assessed against the target local insect pests, L. irrorata grubs under laboratory conditions. The mean mortality (%) caused by H. indica isolate KRR4 on L. irrorata at different dosages (10, 50, 100, 150, 200 and 300 IJs/grub) ranged from 37.5–100% after 24 h of exposure. The LD50 required to kill 50% of the L. irrorata grub population was 5.5 ± 2 IJs/grub. The virulence indicated that local H. indica
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Ramos R. et al. Isolation, identification and virulence of the local entomopathogenic nematode Heterorhabditis indica in the southern Philippines against the sugarcane white grub, Leucopholis irrorata (Chevrolat) (Coleoptera: Scarabaeidae) // International Journal of Tropical Insect Science. 2022. Vol. 42. No. 3. pp. 2739-2750.
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Ramos R., Ann Dichusa C., Aryal S., Crispolon E., Sumaya N. P. D., SUMAYA N. H. N. Isolation, identification and virulence of the local entomopathogenic nematode Heterorhabditis indica in the southern Philippines against the sugarcane white grub, Leucopholis irrorata (Chevrolat) (Coleoptera: Scarabaeidae) // International Journal of Tropical Insect Science. 2022. Vol. 42. No. 3. pp. 2739-2750.
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TY - JOUR
DO - 10.1007/s42690-022-00807-5
UR - https://doi.org/10.1007/s42690-022-00807-5
TI - Isolation, identification and virulence of the local entomopathogenic nematode Heterorhabditis indica in the southern Philippines against the sugarcane white grub, Leucopholis irrorata (Chevrolat) (Coleoptera: Scarabaeidae)
T2 - International Journal of Tropical Insect Science
AU - Ramos, Rolando
AU - Ann Dichusa, Cynthia
AU - Aryal, Sitaram
AU - Crispolon, Elorde
AU - Sumaya, Neil Pep Dave
AU - SUMAYA, NANETTE HOPE N.
PY - 2022
DA - 2022/05/11
PB - Cambridge University Press
SP - 2739-2750
IS - 3
VL - 42
SN - 1742-7584
SN - 1742-7592
ER -
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BibTex (up to 50 authors)
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@article{2022_Ramos,
author = {Rolando Ramos and Cynthia Ann Dichusa and Sitaram Aryal and Elorde Crispolon and Neil Pep Dave Sumaya and NANETTE HOPE N. SUMAYA},
title = {Isolation, identification and virulence of the local entomopathogenic nematode Heterorhabditis indica in the southern Philippines against the sugarcane white grub, Leucopholis irrorata (Chevrolat) (Coleoptera: Scarabaeidae)},
journal = {International Journal of Tropical Insect Science},
year = {2022},
volume = {42},
publisher = {Cambridge University Press},
month = {may},
url = {https://doi.org/10.1007/s42690-022-00807-5},
number = {3},
pages = {2739--2750},
doi = {10.1007/s42690-022-00807-5}
}
Cite this
MLA
Copy
Ramos, Rolando, et al. “Isolation, identification and virulence of the local entomopathogenic nematode Heterorhabditis indica in the southern Philippines against the sugarcane white grub, Leucopholis irrorata (Chevrolat) (Coleoptera: Scarabaeidae).” International Journal of Tropical Insect Science, vol. 42, no. 3, May. 2022, pp. 2739-2750. https://doi.org/10.1007/s42690-022-00807-5.