2-(5-Hydrazinocarbonyl-2-thienyl) -5, 6-methylenedioxybenzofuran and 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran as novel fluorescence derivatisation reagents for carboxylic acids in liquid chromatography
Mikihiko SAITO
1
,
Tamano Ushijima
1
,
Kazumi SASAMOTO
1
,
Kenshi Yakata
1
,
Yosuke OHKURA
1
,
Keiyu UENO
1
1
Dojindo Laboratories, Tabaru 2025-5, Mashiki-machi, Kumamoto 861-22, Japan
|
Publication type: Journal Article
Publication date: 1995-01-01
scimago Q1
wos Q1
SJR: 1.004
CiteScore: 10.4
Impact factor: 6.0
ISSN: 00032670, 18734324
Biochemistry
Spectroscopy
Analytical Chemistry
Environmental Chemistry
Abstract
The new acid hydrazides, 2-(5-hydrazinocarbonyl-2-thienyl)-5,6-methylenedioxybenzofuran (TMBH) and 2-(5-hydrazin-ocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran (FMBH), were synthesized as precolumn fluorescence derivatisation reagents for carboxylic acids in liquid chromatography. These compounds readily react with carboxylic acids in the presence of a coupling reagent under mild conditions in aqueous solution to give fluorescent derivatives. The detection limits of lauric acid were both 0.1 pmol per 10 μl injection volume at a signal-to-noise ratio of 3. The methods in which these compounds are used were applied to the assay of a standard mixture of prostaglandins (25 μM) and prostaglandins in a human seminal fluid.
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SAITO M. et al. 2-(5-Hydrazinocarbonyl-2-thienyl) -5, 6-methylenedioxybenzofuran and 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran as novel fluorescence derivatisation reagents for carboxylic acids in liquid chromatography // Analytica Chimica Acta. 1995. Vol. 300. No. 1-3. pp. 243-251.
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SAITO M., Ushijima T., SASAMOTO K., Yakata K., OHKURA Y., UENO K. 2-(5-Hydrazinocarbonyl-2-thienyl) -5, 6-methylenedioxybenzofuran and 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran as novel fluorescence derivatisation reagents for carboxylic acids in liquid chromatography // Analytica Chimica Acta. 1995. Vol. 300. No. 1-3. pp. 243-251.
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TY - JOUR
DO - 10.1016/0003-2670(94)00422-I
UR - https://doi.org/10.1016/0003-2670(94)00422-I
TI - 2-(5-Hydrazinocarbonyl-2-thienyl) -5, 6-methylenedioxybenzofuran and 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran as novel fluorescence derivatisation reagents for carboxylic acids in liquid chromatography
T2 - Analytica Chimica Acta
AU - SAITO, Mikihiko
AU - Ushijima, Tamano
AU - SASAMOTO, Kazumi
AU - Yakata, Kenshi
AU - OHKURA, Yosuke
AU - UENO, Keiyu
PY - 1995
DA - 1995/01/01
PB - Elsevier
SP - 243-251
IS - 1-3
VL - 300
SN - 0003-2670
SN - 1873-4324
ER -
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@article{1995_SAITO,
author = {Mikihiko SAITO and Tamano Ushijima and Kazumi SASAMOTO and Kenshi Yakata and Yosuke OHKURA and Keiyu UENO},
title = {2-(5-Hydrazinocarbonyl-2-thienyl) -5, 6-methylenedioxybenzofuran and 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran as novel fluorescence derivatisation reagents for carboxylic acids in liquid chromatography},
journal = {Analytica Chimica Acta},
year = {1995},
volume = {300},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016/0003-2670(94)00422-I},
number = {1-3},
pages = {243--251},
doi = {10.1016/0003-2670(94)00422-I}
}
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MLA
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SAITO, Mikihiko, et al. “2-(5-Hydrazinocarbonyl-2-thienyl) -5, 6-methylenedioxybenzofuran and 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran as novel fluorescence derivatisation reagents for carboxylic acids in liquid chromatography.” Analytica Chimica Acta, vol. 300, no. 1-3, Jan. 1995, pp. 243-251. https://doi.org/10.1016/0003-2670(94)00422-I.