Enzymatic synthesis of radioactive (−)-carnitine from γ-butyrobetaine prepared by the methylation of γ-aminobutyric acid
1
Laboratory of Intermediary Metabolism, Clinical Research Institute of Montreal, Montreal H2W 1R7, Quebec, Canada
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Publication type: Journal Article
Publication date: 1982-01-01
scimago Q3
wos Q3
SJR: 0.499
CiteScore: 5.3
Impact factor: 2.5
ISSN: 00032697, 10960309
PubMed ID:
7072950
Biochemistry
Molecular Biology
Cell Biology
Biophysics
Abstract
Radioactive γ-butyrobetaine was prepared by quaternization of γ-aminobutyric acid with tritiated methyl iodide under conditions giving high yields with respect to both the above precursors. Part of the product was passed through a column of ion-retardation resin and gave radioactive γ-butyrobetaine of good purity. The remainder was converted to (−)-carnitine stoichiometrically by employing a 50–60% ammonium sulfate fraction of rat liver supernatant as the source of γ-butyrobetaine hydroxylase (EC 1.14.11.1). Successive column chromatographies on a cation exchanger and ion-retardation resins then gave radioactive (−)-carnitine of good purity in high yield.
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GOST
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Daveluy A., Parvin R., Pande S. V. Enzymatic synthesis of radioactive (−)-carnitine from γ-butyrobetaine prepared by the methylation of γ-aminobutyric acid // Analytical Biochemistry. 1982. Vol. 119. No. 2. pp. 286-292.
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Daveluy A., Parvin R., Pande S. V. Enzymatic synthesis of radioactive (−)-carnitine from γ-butyrobetaine prepared by the methylation of γ-aminobutyric acid // Analytical Biochemistry. 1982. Vol. 119. No. 2. pp. 286-292.
Cite this
RIS
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TY - JOUR
DO - 10.1016/0003-2697(82)90587-5
UR - https://doi.org/10.1016/0003-2697(82)90587-5
TI - Enzymatic synthesis of radioactive (−)-carnitine from γ-butyrobetaine prepared by the methylation of γ-aminobutyric acid
T2 - Analytical Biochemistry
AU - Daveluy, Albert
AU - Parvin, Rehana
AU - Pande, Shri V.
PY - 1982
DA - 1982/01/01
PB - Elsevier
SP - 286-292
IS - 2
VL - 119
PMID - 7072950
SN - 0003-2697
SN - 1096-0309
ER -
Cite this
BibTex (up to 50 authors)
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@article{1982_Daveluy,
author = {Albert Daveluy and Rehana Parvin and Shri V. Pande},
title = {Enzymatic synthesis of radioactive (−)-carnitine from γ-butyrobetaine prepared by the methylation of γ-aminobutyric acid},
journal = {Analytical Biochemistry},
year = {1982},
volume = {119},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016/0003-2697(82)90587-5},
number = {2},
pages = {286--292},
doi = {10.1016/0003-2697(82)90587-5}
}
Cite this
MLA
Copy
Daveluy, Albert, et al. “Enzymatic synthesis of radioactive (−)-carnitine from γ-butyrobetaine prepared by the methylation of γ-aminobutyric acid.” Analytical Biochemistry, vol. 119, no. 2, Jan. 1982, pp. 286-292. https://doi.org/10.1016/0003-2697(82)90587-5.