Elsevier
Analytical Biochemistry
,  том 147 ,  издание 2 ,  страницы 497-502

One-step fluorometric microassay of DNA in procaryotes

Тип публикации: Journal Article
Дата публикации: 1985-06-01
Elsevier
scimago Q3
wos Q3
БС2
SJR: 0.499
CiteScore: 5.3
Impact factor: 2.5
ISSN: 00032697, 10960309
Biochemistry
Molecular Biology
Cell Biology
Biophysics
Краткое описание
A fluorometric DNA microassay in procaryotes is proposed. The fluorescent dye employed is 4',6-diamidino-2-phenylindole X 2HCl, which exhibits very high specificity for DNA. The assay can be directly made on whole bacteria without DNA purification. Bacteria are treated with toluene and the fluorescent dye is added. The range of linearity has been explored. The stability, reproducibility, and accuracy of fluorescence measurements permit one to determine the equivalent number of genomes per cell and to distinguish between two different but very similar modes of increase in DNA quantity: bilinear or exponential synthesis.
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ГОСТ |
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Legros M., Kepes A. One-step fluorometric microassay of DNA in procaryotes // Analytical Biochemistry. 1985. Vol. 147. No. 2. pp. 497-502.
ГОСТ со всеми авторами (до 50) Скопировать
Legros M., Kepes A. One-step fluorometric microassay of DNA in procaryotes // Analytical Biochemistry. 1985. Vol. 147. No. 2. pp. 497-502.
RIS |
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TY - JOUR
DO - 10.1016/0003-2697(85)90304-5
UR - https://doi.org/10.1016/0003-2697(85)90304-5
TI - One-step fluorometric microassay of DNA in procaryotes
T2 - Analytical Biochemistry
AU - Legros, Marcel
AU - Kepes, Adam
PY - 1985
DA - 1985/06/01
PB - Elsevier
SP - 497-502
IS - 2
VL - 147
PMID - 3893219
SN - 0003-2697
SN - 1096-0309
ER -
BibTex |
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BibTex (до 50 авторов) Скопировать
@article{1985_Legros,
author = {Marcel Legros and Adam Kepes},
title = {One-step fluorometric microassay of DNA in procaryotes},
journal = {Analytical Biochemistry},
year = {1985},
volume = {147},
publisher = {Elsevier},
month = {jun},
url = {https://doi.org/10.1016/0003-2697(85)90304-5},
number = {2},
pages = {497--502},
doi = {10.1016/0003-2697(85)90304-5}
}
MLA
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MLA Скопировать
Legros, Marcel, and Adam Kepes. “One-step fluorometric microassay of DNA in procaryotes.” Analytical Biochemistry, vol. 147, no. 2, Jun. 1985, pp. 497-502. https://doi.org/10.1016/0003-2697(85)90304-5.