Elsevier
Analytical Biochemistry
,  volume 147 ,  issue 2 ,  pages 497-502

One-step fluorometric microassay of DNA in procaryotes

Publication type: Journal Article
Publication date: 1985-06-01
Elsevier
scimago Q3
wos Q3
SJR: 0.499
CiteScore: 5.3
Impact factor: 2.5
ISSN: 00032697, 10960309
Biochemistry
Molecular Biology
Cell Biology
Biophysics
Abstract
A fluorometric DNA microassay in procaryotes is proposed. The fluorescent dye employed is 4',6-diamidino-2-phenylindole X 2HCl, which exhibits very high specificity for DNA. The assay can be directly made on whole bacteria without DNA purification. Bacteria are treated with toluene and the fluorescent dye is added. The range of linearity has been explored. The stability, reproducibility, and accuracy of fluorescence measurements permit one to determine the equivalent number of genomes per cell and to distinguish between two different but very similar modes of increase in DNA quantity: bilinear or exponential synthesis.
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GOST |
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GOST Copy
Legros M., Kepes A. One-step fluorometric microassay of DNA in procaryotes // Analytical Biochemistry. 1985. Vol. 147. No. 2. pp. 497-502.
GOST all authors (up to 50) Copy
Legros M., Kepes A. One-step fluorometric microassay of DNA in procaryotes // Analytical Biochemistry. 1985. Vol. 147. No. 2. pp. 497-502.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/0003-2697(85)90304-5
UR - https://doi.org/10.1016/0003-2697(85)90304-5
TI - One-step fluorometric microassay of DNA in procaryotes
T2 - Analytical Biochemistry
AU - Legros, Marcel
AU - Kepes, Adam
PY - 1985
DA - 1985/06/01
PB - Elsevier
SP - 497-502
IS - 2
VL - 147
PMID - 3893219
SN - 0003-2697
SN - 1096-0309
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{1985_Legros,
author = {Marcel Legros and Adam Kepes},
title = {One-step fluorometric microassay of DNA in procaryotes},
journal = {Analytical Biochemistry},
year = {1985},
volume = {147},
publisher = {Elsevier},
month = {jun},
url = {https://doi.org/10.1016/0003-2697(85)90304-5},
number = {2},
pages = {497--502},
doi = {10.1016/0003-2697(85)90304-5}
}
MLA
Cite this
MLA Copy
Legros, Marcel, and Adam Kepes. “One-step fluorometric microassay of DNA in procaryotes.” Analytical Biochemistry, vol. 147, no. 2, Jun. 1985, pp. 497-502. https://doi.org/10.1016/0003-2697(85)90304-5.