Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines.
1
Istituto per la Chimica di Molecole di Interesse Biologico, C. N. R., Naples, Italy.
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2
Istituto per la Chimica di Molecole di Interesse Biologico, C.N.R. Via Toiano, 6 80072 Arco Felice Naples, Italy,
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Publication type: Journal Article
Publication date: 1995-10-01
scimago Q2
wos Q2
SJR: 0.856
CiteScore: 6.1
Impact factor: 3.2
ISSN: 09523278, 15322823
PubMed ID:
8577784
Cell Biology
Clinical Biochemistry
Abstract
Recent reports have suggested that N-arachidonoylethanolamine (anandamide) acts as an endogenous ligand for cannabinoid receptors in mammalian brain. Here we describe methods for the extraction, purification and analysis of anandamide and related N-fatty acyl-ethanolamines (NAEs). Liquid-phase extraction, silica gel G column chromatography and thin-layer chromatography (TLC) were employed for sample fractionation. Three analytical high-performance liquid chromatography (HPLC) methods for purification of NAEs were developed. Finally, analyses of NAEs by gas chromatography/mass spectrometry (GC/MS) are described. The applications of these analytical methods to the identification of anandamide and related NAEs in cell cultures as well as of artifacts in biosynthetic studies are described.
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Fontana A. et al. Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines. // Prostaglandins Leukotrienes and Essential Fatty Acids. 1995. Vol. 53. No. 4. pp. 301-308.
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Fontana A., Di Marzo V., Cadas H., PIOMELLI D. Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines. // Prostaglandins Leukotrienes and Essential Fatty Acids. 1995. Vol. 53. No. 4. pp. 301-308.
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TY - JOUR
DO - 10.1016/0952-3278(95)90130-2
UR - https://doi.org/10.1016/0952-3278(95)90130-2
TI - Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines.
T2 - Prostaglandins Leukotrienes and Essential Fatty Acids
AU - Fontana, A.
AU - Di Marzo, Vincenzo
AU - Cadas, H
AU - PIOMELLI, D.
PY - 1995
DA - 1995/10/01
PB - Elsevier
SP - 301-308
IS - 4
VL - 53
PMID - 8577784
SN - 0952-3278
SN - 1532-2823
ER -
Cite this
BibTex (up to 50 authors)
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@article{1995_Fontana,
author = {A. Fontana and Vincenzo Di Marzo and H Cadas and D. PIOMELLI},
title = {Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines.},
journal = {Prostaglandins Leukotrienes and Essential Fatty Acids},
year = {1995},
volume = {53},
publisher = {Elsevier},
month = {oct},
url = {https://doi.org/10.1016/0952-3278(95)90130-2},
number = {4},
pages = {301--308},
doi = {10.1016/0952-3278(95)90130-2}
}
Cite this
MLA
Copy
Fontana, A., et al. “Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines..” Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 53, no. 4, Oct. 1995, pp. 301-308. https://doi.org/10.1016/0952-3278(95)90130-2.