volume 53 issue 4 pages 301-308

Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines.

A. Fontana 1
H Cadas 3
D. PIOMELLI 3
1
 
Istituto per la Chimica di Molecole di Interesse Biologico, C. N. R., Naples, Italy.
2
 
Istituto per la Chimica di Molecole di Interesse Biologico, C.N.R. Via Toiano, 6 80072 Arco Felice Naples, Italy,
Publication typeJournal Article
Publication date1995-10-01
scimago Q2
wos Q2
SJR0.856
CiteScore6.1
Impact factor3.2
ISSN09523278, 15322823
Cell Biology
Clinical Biochemistry
Abstract
Recent reports have suggested that N-arachidonoylethanolamine (anandamide) acts as an endogenous ligand for cannabinoid receptors in mammalian brain. Here we describe methods for the extraction, purification and analysis of anandamide and related N-fatty acyl-ethanolamines (NAEs). Liquid-phase extraction, silica gel G column chromatography and thin-layer chromatography (TLC) were employed for sample fractionation. Three analytical high-performance liquid chromatography (HPLC) methods for purification of NAEs were developed. Finally, analyses of NAEs by gas chromatography/mass spectrometry (GC/MS) are described. The applications of these analytical methods to the identification of anandamide and related NAEs in cell cultures as well as of artifacts in biosynthetic studies are described.
Found 
Found 

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Fontana A. et al. Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines. // Prostaglandins Leukotrienes and Essential Fatty Acids. 1995. Vol. 53. No. 4. pp. 301-308.
GOST all authors (up to 50) Copy
Fontana A., Di Marzo V., Cadas H., PIOMELLI D. Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines. // Prostaglandins Leukotrienes and Essential Fatty Acids. 1995. Vol. 53. No. 4. pp. 301-308.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/0952-3278(95)90130-2
UR - https://doi.org/10.1016/0952-3278(95)90130-2
TI - Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines.
T2 - Prostaglandins Leukotrienes and Essential Fatty Acids
AU - Fontana, A.
AU - Di Marzo, Vincenzo
AU - Cadas, H
AU - PIOMELLI, D.
PY - 1995
DA - 1995/10/01
PB - Elsevier
SP - 301-308
IS - 4
VL - 53
PMID - 8577784
SN - 0952-3278
SN - 1532-2823
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{1995_Fontana,
author = {A. Fontana and Vincenzo Di Marzo and H Cadas and D. PIOMELLI},
title = {Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines.},
journal = {Prostaglandins Leukotrienes and Essential Fatty Acids},
year = {1995},
volume = {53},
publisher = {Elsevier},
month = {oct},
url = {https://doi.org/10.1016/0952-3278(95)90130-2},
number = {4},
pages = {301--308},
doi = {10.1016/0952-3278(95)90130-2}
}
MLA
Cite this
MLA Copy
Fontana, A., et al. “Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines..” Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 53, no. 4, Oct. 1995, pp. 301-308. https://doi.org/10.1016/0952-3278(95)90130-2.