Elsevier
Analytical Biochemistry
,  volume 629 ,  pages 114294

Modification of a spectrophotometric method for assessment of monoamine oxidase activity with 2,4-dinitrophenylhydrazine as a derivatizing reagent

Publication type: Journal Article
Publication date: 2021-09-01
Elsevier
scimago Q3
wos Q3
SJR: 0.499
CiteScore: 5.3
Impact factor: 2.5
ISSN: 00032697, 10960309
Biochemistry
Molecular Biology
Cell Biology
Biophysics
Abstract
The aim of the study was to modify a simple and widely used spectrophotometric assay for MAO activity evaluation with 2,4-dinitrophenylhydrazine. A modified procedure includes molar absorption coefficients of 2,4-DNP-hydrazone benzaldehyde and 2,4-DNP-hydrazone 5-hydroxyindolylacetaldehyde as 2.3 × 104mol-1l cm-1 and 1.0 × 104 mol-1l cm-1, respectively. Such an approach allows to express specific enzyme activity as nmol product formed/min/mg protein.
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Vinel P. K. et al. Modification of a spectrophotometric method for assessment of monoamine oxidase activity with 2,4-dinitrophenylhydrazine as a derivatizing reagent // Analytical Biochemistry. 2021. Vol. 629. p. 114294.
GOST all authors (up to 50) Copy
Vinel P. K., Grobovoy S. I., Sinitskii A. I., Kolesnikov O. L. Modification of a spectrophotometric method for assessment of monoamine oxidase activity with 2,4-dinitrophenylhydrazine as a derivatizing reagent // Analytical Biochemistry. 2021. Vol. 629. p. 114294.
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RIS Copy
TY - JOUR
DO - 10.1016/j.ab.2021.114294
UR - https://doi.org/10.1016/j.ab.2021.114294
TI - Modification of a spectrophotometric method for assessment of monoamine oxidase activity with 2,4-dinitrophenylhydrazine as a derivatizing reagent
T2 - Analytical Biochemistry
AU - Vinel, Polina K
AU - Grobovoy, S I
AU - Sinitskii, A I
AU - Kolesnikov, O L
PY - 2021
DA - 2021/09/01
PB - Elsevier
SP - 114294
VL - 629
PMID - 34197779
SN - 0003-2697
SN - 1096-0309
ER -
BibTex
Cite this
BibTex (up to 50 authors) Copy
@article{2021_Vinel,
author = {Polina K Vinel and S I Grobovoy and A I Sinitskii and O L Kolesnikov},
title = {Modification of a spectrophotometric method for assessment of monoamine oxidase activity with 2,4-dinitrophenylhydrazine as a derivatizing reagent},
journal = {Analytical Biochemistry},
year = {2021},
volume = {629},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016/j.ab.2021.114294},
pages = {114294},
doi = {10.1016/j.ab.2021.114294}
}