volume 44 issue 7 pages 1829-1838

Preliminary investigations of spider silk in wounds in vivo — Implications for an innovative wound dressing

C. Liebsch 1
V. Bucan 1
Bjoern Menger 1
Franziska Köhne 1
Karl-Heinz Waldmann 2
Desiree Vaslaitis 1
Peter M. Vogt 1
Sarah Strauss 1
Joern W. Kuhbier 1
Publication typeJournal Article
Publication date2018-11-01
scimago Q1
wos Q1
SJR0.764
CiteScore4.7
Impact factor2.9
ISSN03054179, 18791409
General Medicine
Surgery
Critical Care and Intensive Care Medicine
Emergency Medicine
Abstract
The ideal wound dressing in particular for burn wounds has not been found yet. The aim of this study was to investigate native spider silk as a novel wound dressing. Release of inflammatory cytokines of macrophages and neutrophile granulocytes was determined via ELISA after exposure to spider silk. Migration of dermal cells as well as angiogenesis on spider silk was visualized with live video microscopy or chorioallantois membrane model, respectively. Native spider silk was placed in full-thickness skin wounds in a sheep in vivo-model and wounds were evaluated after 2, 4, 6, and 8weeks histologically as well as per quantitative real-time PCR. Minimal inflammatory cytokine release could be seen for spider silk. Ingrowth of single capillaries into bundles of spider silk and migration of keratinocytes as well as fibroblasts on spider silk fibres was proven. Macroscopically, a comparable wound closure could be seen in spider silk and in sham controls. In histological evaluation, a thicker epidermis was observed in spider silk treated wounds while collagen III/I expression ratio was comparable in both groups. As native spider silk has been described as highly biocompatible, it might represent an innovative alternative to common wound dressings.
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GOST Copy
Liebsch C. et al. Preliminary investigations of spider silk in wounds in vivo — Implications for an innovative wound dressing // Burns. 2018. Vol. 44. No. 7. pp. 1829-1838.
GOST all authors (up to 50) Copy
Liebsch C., Bucan V., Menger B., Köhne F., Waldmann K., Vaslaitis D., Vogt P. M., Strauss S., Kuhbier J. W. Preliminary investigations of spider silk in wounds in vivo — Implications for an innovative wound dressing // Burns. 2018. Vol. 44. No. 7. pp. 1829-1838.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/j.burns.2018.03.016
UR - https://doi.org/10.1016/j.burns.2018.03.016
TI - Preliminary investigations of spider silk in wounds in vivo — Implications for an innovative wound dressing
T2 - Burns
AU - Liebsch, C.
AU - Bucan, V.
AU - Menger, Bjoern
AU - Köhne, Franziska
AU - Waldmann, Karl-Heinz
AU - Vaslaitis, Desiree
AU - Vogt, Peter M.
AU - Strauss, Sarah
AU - Kuhbier, Joern W.
PY - 2018
DA - 2018/11/01
PB - Elsevier
SP - 1829-1838
IS - 7
VL - 44
PMID - 30057335
SN - 0305-4179
SN - 1879-1409
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2018_Liebsch,
author = {C. Liebsch and V. Bucan and Bjoern Menger and Franziska Köhne and Karl-Heinz Waldmann and Desiree Vaslaitis and Peter M. Vogt and Sarah Strauss and Joern W. Kuhbier},
title = {Preliminary investigations of spider silk in wounds in vivo — Implications for an innovative wound dressing},
journal = {Burns},
year = {2018},
volume = {44},
publisher = {Elsevier},
month = {nov},
url = {https://doi.org/10.1016/j.burns.2018.03.016},
number = {7},
pages = {1829--1838},
doi = {10.1016/j.burns.2018.03.016}
}
MLA
Cite this
MLA Copy
Liebsch, C., et al. “Preliminary investigations of spider silk in wounds in vivo — Implications for an innovative wound dressing.” Burns, vol. 44, no. 7, Nov. 2018, pp. 1829-1838. https://doi.org/10.1016/j.burns.2018.03.016.