Daudi, Dauddin Ilyasovich

Sorochanu I.P., Blitzine K.S., Daudi D.I., Zhemkov N.I., Pechenina A.A., Dmitrieva M.A., Grin N.A., Asatryan T.T., Tatarkin V.V., Trunin E.M., Deev R.V.

BACKGROUND: Disruption of the stages of reparative regeneration leads to insufficient formation of the extracellular matrix and the development of chronic wounds, which require a personalized therapeutic approach. Modern regenerative medicine utilizes biopolymers, such as silk proteins, as the basis for wound dressings and drug delivery systems, which possess a number of unique properties. The biocompatibility, influence on intracellular signaling pathways, and antibacterial activity of spidroin (spider silk protein), fibroin, and sericin (the basis of insect silk) make them potentially applicable as wound healing agents. AIM: The aim of the study is to characterize the effect of a cream based on a solution of silk spidroin, fibroin, and sericin proteins on skin regeneration in a comparative experiment. MATERIALS AND METHODS: The study was conducted on 30 male rats with surgically created full-thickness excisional skin defects measuring 20 mm in diameter on their backs. The rats were then divided into three groups: group 1 received daily topical application of the investigated cream, group 2 received a commercial panthenol product, group 3 served as the control group, with wounds left to heal spontaneously. The process of reparative regeneration and reactive changes were assessed through clinical blood analysis, planimetric measurements of wound closure, and histomorphometric analysis of tissue samples. RESULTS: The treatment group exhibited a positive trend in wound healing (p=0.010) with faster complete skin closure (by day 14) compared to the control group. Evaluation of the inflammatory response revealed no significant changes in blood parameters (moderate granulocytosis and signs of acute posthemorrhagic anemia) and reduced immune cell infiltration compared to the control group. CONCLUSION: The combination of spider silk proteins (spidroin) and insect silk proteins (fibroin and sericin) has the potential to enhance cell migration, proliferation, and differentiation, as well as extracellular matrix production. They also exhibit anti-inflammatory activity without possessing immunogenic properties. These characteristics provide a basis for the potential use of these proteins as standalone therapeutic agents, including in the clinical management of wounds with impaired healing.

Vanchugov I.M., Daudi D.I., Rezanov K.S., Shestakov R.A., Barakov V.V.

Tonkonogov B.P., Kilyakova A.Y., Daudi D.I., Spiridonova A.D., Krylova A.Y., Safieva R.Z.

The possibility of using biochar of plant origin as filler in polyurea plastic greases was studied, and its effect on their performance was investigated. In the preparation of the greases petroleum oil of group III and dioctyl adipate were used as dispersion medium, and 20% diurea was used an thickener. It was found that greases containing 5-15 wt. % of biochar reduce the diameter of the wear scar better and have higher colloidal stability than samples not containing biochar. The performance of the grease is improved by the addition of 1 wt. % of polyisobutylene thickener PIB 10 to the dispersion medium.

Daudi D.I., Kilyakova A.Y., Kalyanova O.A., Korotaev A.F., Aleksanyan K.G.

The purpose of this work was to improve the tribological characteristics and low-temperature properties of polyurea greases, as well as to expand the operating temperatures of their use. In this work, as the dispersion medium were used base oils of groups I (I-20) and III (VHVI-4). Diurea was used as a thickener. The obtained samples of the lubricating greases were analyzed: penetration (GOST 32331-2013), drop-point temperature (GOST 32394-2013), colloidal stability (GOST 7142-74), tribological characteristics (GOST 9490-75), and effective viscosity at -50ºС (GOST 26581-85). Based on the analysis of the obtained data, it was concluded that the best properties have lubricants prepared on the group IIIbase oils. To improve the low-temperature properties, it was proposed to add a 50% ester (DOTF) to the dispersion medium of greases which have better performance.As a result of work done, we concluded that the polyurea greases improved their tribological characteristics and low-temperature properties after adding ether to VHVI-4 (1:1) as a dispersion medium.

Khakimov A.R., Daudi D.I., Ishmurzin A.A., Kodryanu N.P.

Summary One of the most significant problems of GPR in the study of roads is a large number of low-contrast boundaries that need to be traced at depths of 5–10 m with a radarogram resolution of 10–20 cm. Within each layer, interlayers with a thickness of several centimeters can be distinguished, the presence of which, with sufficient the resolution of radarograms can be indirectly established by the wave field between extended reflective boundaries. Separation of closely spaced reflected and diffracted waves on radarograms is an important task of GPR. Deconvolution is aimed at increasing the resolution of GPR. The paper shows the results of applying deconvolution to ground-penetrating radar data in the study of highways.

Daudi D.I., Kilyakova A.Y.

Summary Engineering and geological conditions are determined by the structure, composition and properties of sediments (soils) of the upper part of the section. In order to identify and assess possible geological complications during the drilling of the bottom part of the section, along with areal engineering surveys, 3D seismic data are successfully used.

Kozdrach R., Radulski P.

Effect of modifying a vegetable grease with different amounts of chokeberry biocarbon on its tribological and rheological properties. The aim of the research was to investigate the effect of the incorporation of biochar derived from the pyrolysis of chokeberry biomass at 500 and 700 °C respectively, in lubricants formulated with vegetable oil, on their functional characteristics in terms of tribology and rheology. Fumed silicon dioxide was utilized as a thickening agent, ensuring the production of lubricants with a consistency class of II. The biochar was introduced into the lubricating formulations in concentrations of 1, 3, and 5%. A rapeseed-based grease with commercial activated carbon served as the reference sample. The research focused on assessing how this innovative additive influenced both the tribological and rheological behaviors of the resulting lubricants. The study evaluated the effects of chokeberry-derived biochar on the wear resistance and scuffing prevention properties of the vegetable-based greases. Additionally, the biochar’s influence on various rheological parameters such as flow curves, viscosity profiles, hysteresis loops, and viscoelastic characteristics was analyzed. Results demonstrated that the biochar additive considerably enhanced the tribological and rheological performance of the tested rapeseed-based grease.

Molza A., Cesaire T., Bercion Y., Thomas P.

In Caribbean islands, the washing ashore of tons of pelagic Sargassum spp., consisting of two species Sargassum fluitans and Sargassum natans, has been regularly occurring since 2011. As green lubrication is a growing trend in the tribology industry, biochar is a promising alternative. Sargassum biochars, produced from Sargassum pelagic algae, are therefore being studied as solid lubricants. This study aims to explore their potential applications. Biochars from brown algae were pyrolyzed at 400 °C and then annealed at different temperatures (from 600 °C to 1500 °C). The Raman spectra collected on the different biochars showed that there was a structural organization of the biochars as the temperature increased. The tribologic properties of the biochars were studied and compared to a solid lubricant reference (exfoliated graphite). Raman spectroscopy analysis revealed a progressive structural reorganization with increasing temperature, leading to a 58% reduction in the coefficient of friction. The morphology and the structure of the tribofilm are investigated by profilometry, scanning electron microscopy, and Raman microspectrometry. Overall, these results can be considered as a first step for utilizing the biochar derived from brown algae Sargassum sp. as an additive in the lubricant industry, for the purpose of emission reduction.

Wang Y., Hou X., Zhang L., Ali M.K., Jiang H., Ma Y.

Nano-materials, given their excellent anti-friction and anti-wear characteristics, have attracted significant attention in tribological applications. However, conventional nano-materials have difficulty meeting the development requirements of the eco-friendly lubricants. In this study, nano biochar derived from sesame stalk was synthesized and used as a lubricant additive in poly-alpha olefin-6 (PAO6). The tribological properties were evaluated by four ball tribological tests with steel and ceramic balls as the friction pair. Following the friction experiment, the worn surface on the steel ball was analyzed by X-ray photoelectron spectrometry. The steel ball friction results showed that the lubricants containing 0.2 wt% nano biochar achieved a maximum wear volume reduction of 46% compared to PAO6, and the friction coefficient decreased by 34.6% for the ceramic balls. Analysis indicated that the enhanced wettability of nano biochar lubricant contributed to improving the anti-wear properties. Characterization of the worn surface revealed that the addition of nano biochar facilitated the formation of strong Fe3O4 tribofilms. This study demonstrated that nano biochar could serve as an environmentally friendly additive in the liquid lubricant industry.

Sen C.K.

Mazurek Ł., Szudzik M., Rybka M., Konop M.

The skin, acting as the outer protection of the human body, is most vulnerable to injury. Wound healing can often be impaired, leading to chronic, hard-to-heal wounds. For this reason, searching for the most effective dressings that can significantly enhance the wound healing process is necessary. In this regard, silk fibroin, a protein derived from silk fibres that has excellent properties, is noteworthy. Silk fibroin is highly biocompatible and biodegradable. It can easily make various dressings, which can be loaded with additional substances to improve healing. Dressings based on silk fibroin have anti-inflammatory, pro-angiogenic properties and significantly accelerate skin wound healing, even compared to commercially available wound dressings. Animal studies confirm the beneficial influence of silk fibroin in wound healing. Clinical research focusing on fibroin dressings is also promising. These properties make silk fibroin a remarkable natural material for creating innovative, simple, and effective dressings for skin wound healing. In this review, we summarise the application of silk fibroin biomaterials as wound dressings in full-thickness, burn, and diabetic wounds in preclinical and clinical settings.

Abd El-Aziz F.E., Hetta H.F., Abdelhamid B.N., Abd Ellah N.H.

Aim: The essential protein element of spider silk ‘spidroin’ was used to assess its impact on the wound-healing process. Methods: Spidroin nanoparticles (NPs) were prepared using poly(lactic-co-glycolic acid) polymer (PLGA/spidroin NPs) at different weight ratios (5:1, 10:1, 15:1) and were in vitro characterized. The optimized NPs were tested in vitro for release and antibacterial activity. To assess wound-healing effects, NPs were topically applied on surgically induced injuries in Allolobophora caliginosa earthworms as a robust human skin model. Results: Optimized NPs (173 ± 3 nm) revealed considerable antibacterial effect against Staphylococcus aureus and Escherichia coli. After 4 days of NPs application on wounds, macroscopical and histological examinations revealed a significant reduction in wound and re-epithelialization times. Conclusion: PLGA/spidroin NPs may represent a promising option for wound repair.

Liu Y., Huang W., Meng M., Chen M., Cao C.

Spider silk protein has attracted much attention on account of its excellent mechanical properties, biodegradability, and biocompatibility. As the main protein component of spider silk, spidroin plays important role in spider spinning under natural circumstances and biomaterial application in medicine as well. Compare to the native spidroin which has a large molecular weight (>300 kDa) with highly repeat glycine and polyalanine regions, the recombinant spidroin was maintained the core amino motifs and much easier to collect. Here, we reviewed the application of recombinant spider silk protein eADF4(C16), major ampullate spidroin (MaSp), minor ampullate spidroin (MiSp), and the derivatives of recombinant spider silk protein in drug delivery system. Moreover, we also reviewed the application of spider silk protein in the field of alternative materials, repairing materials, wound dressing, surgical sutures along with advances in recombinant spider silk protein.

Shitole M., Dugam S., Tade R., Nangare S.

Silk sericin is an applicable protein extracted from dried silk cocoons, which are composed of a polar amino acid chain with hydroxyl, carboxyl, and amino functional groups. It anticipated that the functionality present in sericin provides excellent assets such as stability, release-modifying capacity, interaction with molecules, etc. It has various significant properties like biocompatibility, biodegradability, non-toxicity, hydrophilicity, etc. Besides, sericin exhibits the capability to form gels, nanoparticles, micro-particles, and keen bioengineered materials. Sericin also offers stability by strong affinity to the drug substance/molecules. Currently, sericin reconnoitered for a variety of pharmaceutical applications including solubility enhancement, release modifications, formulation stabilization, and as a drug carrier, etc. The focus of the review is to deliver an impression of the sericin based pharmaceutical applications including nanocrystal and nanoparticle stabilization, inhibition of devitrification, solubility enhancement, controlled and the extended-release formulation, targeted drug delivery, wound healing applications and as a suitable carrier.

Cifuentes A., Gómez-Gil V., Ortega M.A., Asúnsolo Á., Coca S., Román J.S., Álvarez-Mon M., Buján J., García-Honduvilla N.

• Functionalized hydrogels improved diabetes-associated impaired wound healing. • Bemiparin showed a greater capacity for collagen maturation and contributes to the stabilization of neoformed scar tissue. • Chitosan hydrogels promote the progression of the reparative process and induce the formation of stable scar tissue. • Quality of scar tissue at 21 days is clearly better and this would lead us, to a lower recurrence of ulcers. Diabetes mellitus causes severe impairment in the cutaneous wound healing process, which has led to extensive research striving to establish new treatments. In this work, we describe the effects of chitosan hydrogels functionalized with either unfractionated heparin or bemiparin (a low molecular weight heparin, LMWH) as topical treatments in an experimental diabetic wound healing model. Although wound morphometry showed similar values at the end of the study, microscopic analyses revealed impaired healing in diabetic animals in terms of inflammation and tissue formation. However, both types of loaded hydrogels accelerated inflammation resolution and improved the epithelialization process, while showing a neodermal thickness similar to that of nondiabetic animals. Immunohistochemistry analyses revealed an intermediate response in macrophage evolution between diabetic and nondiabetic controls in the treated groups, as well as enhanced collagenization and myofibroblast progression patterns. However, these changes were not accompanied by differences among groups in collagen I, III and TGF-β1 gene expression. Functionalized hydrogels improved diabetes-associated impaired wound healing, thus promoting the progression of the process and inducing the formation of high-quality cicatricial tissue. Although the beneficial healing effect observed after topical treatment with chitosan hydrogels loaded with bemiparin or unfractionated heparin was similar, the chitosan hydrogel loaded with bemiparin is the preferred choice as it exhibited high-quality tissue in the neoformed dermal tissue.

Gorbacheva S.N., Yarmush Y.M., Ilyin S.O.

Due to the instability of the dispersions of cellulose particles in non-polar esters, they cannot be considered for use as lubricants or for other purposes. However, such dispersions can be made stable by addition a third component that can create a structural network to prevent aggregation and sedimentation of the cellulose particles. A series of di(2-ethylhexyl) sebacate greases have been obtained using microcrystalline cellulose as a thickener and organomodified montmorillonite as a stabilizer. The characteristics of physicochemical properties of greases have been obtained: yield stress, elastic modulus, friction and wear coefficients, and wear scar diameter under heavy loads were measured. The effect of component concentration and mixing conditions on the rheological and tribological parameters was shown.

Adolfsson K.H., Yadav N., Hakkarainen M.

Hydrothermally carbonized cellulose and its further modifications are intriguing materials for a wide range of potential applications. Hydrothermal carbonization is a sustainable process for conver ...

Shi C., Wang C., Liu H., Li Q., Li R., Zhang Y., Liu Y., Shao Y., Wang J.

There are many factors involved in wound healing, and the healing process is not static. The therapeutic effect of modern wound dressings in the clinical management of wounds is documented. However, there are few reports regarding the reasonable selection of dressings for certain types of wounds in the clinic. In this article, we retrospect the history of wound dressing development and the classification of modern wound dressings. In addition, the pros and cons of mainstream modern wound dressings for the healing of different wounds, such as diabetic foot ulcers, pressure ulcers, burns and scalds, and chronic leg ulcers, as well as the physiological mechanisms involved in wound healing are summarized. This article provides a clinical guideline for selecting suitable wound dressings according to the types of wounds.

Gholipourmalekabadi M., Sapru S., Samadikuchaksaraei A., Reis R.L., Kaplan D.L., Kundu S.C.

Several synthetic and natural materials are used in soft tissue engineering and regenerative medicine with varying degrees of success. Among them, silkworm silk protein fibroin, a naturally occurring protein-based biomaterial, exhibits many promising characteristics such as biocompatibility, controllable biodegradability, tunable mechanical properties, aqueous preparation, minimal inflammation in host tissue, low cost and ease of use. Silk fibroin is often used alone or in combination with other materials in various formats and is also a promising delivery system for bioactive compounds as part of such repair scenarios. These properties make silk fibroin an excellent biomaterial for skin tissue engineering and repair applications. This review focuses on the promising characteristics and recent advances in the use of silk fibroin for skin wound healing and/or soft-tissue repair applications. The benefits and limitations of silk fibroin as a scaffolding biomaterial in this context are also discussed. STATEMENT OF SIGNIFICANCE: Silk protein fibroin is a natural biomaterial with important biological and mechanical properties for soft tissue engineering applications. Silk fibroin is obtained from silkworms and can be purified using alkali or enzyme based degumming (removal of glue protein sericin) procedures. Fibroin is used alone or in combination with other materials in different scaffold forms, such as nanofibrous mats, hydrogels, sponges or films tailored for specific applications. The investigations carried out using silk fibroin or its blends in skin tissue engineering have increased dramatically in recent years due to the advantages of this unique biomaterial. This review focuses on the promising characteristics of silk fibroin for skin wound healing and/or soft-tissue repair applications.

Tonkonogov B.P., Kilyakova A.Y., Stenina N.D., Popova O.V., Safieva R.Z., Vinokurov V.A., Gorbacheva S.N., Il’in S.O.

Polyurea lubricant composition with conditionally two and four urea units per polyurea thickener molecule in the presence of a second biodegradable thickener (nanocellulose, cetearyl alcohol) were synthesized. The drop point, penetration, and oil separation and rheological, tribological, and antiwear properties were evaluated. The lubricant composition properties changed depending on the number of urea units per polyurea molecule and content of second biodegradable thickener. Tetraurea-based greases had lower viscosity and better tribological and antiwear properties regardless of the nanocellulose content, confirming that the urea-thickener structure had a decisive influence on the lubricant-composition properties.

Maksimova Y.M., Shakhmatova A.S., Ilyin S.O., Pakhmanova O.A., Lyadov A.S., Antonov S.V., Parenago O.P.

Lubricating greases based on pentaerythritol and trimethylolpropane esters and dioctyl sebacate have been synthesized using diureas as a thickener prepared by reactions of toluene diisocyanate, aniline, and primary aliphatic amines of various structures. The physicochemical properties of the greases, such as ultimate strength, yield stress, modulus of elasticity, dropping point, and colloid stability, have been characterized, and data on their antiwear activity have been obtained. The relationship between the structure of grease components, its rheology, and antiwear properties has been revealed.

Liebsch C., Bucan V., Menger B., Köhne F., Waldmann K., Vaslaitis D., Vogt P.M., Strauss S., Kuhbier J.W.

The ideal wound dressing in particular for burn wounds has not been found yet. The aim of this study was to investigate native spider silk as a novel wound dressing. Release of inflammatory cytokines of macrophages and neutrophile granulocytes was determined via ELISA after exposure to spider silk. Migration of dermal cells as well as angiogenesis on spider silk was visualized with live video microscopy or chorioallantois membrane model, respectively. Native spider silk was placed in full-thickness skin wounds in a sheep in vivo-model and wounds were evaluated after 2, 4, 6, and 8weeks histologically as well as per quantitative real-time PCR. Minimal inflammatory cytokine release could be seen for spider silk. Ingrowth of single capillaries into bundles of spider silk and migration of keratinocytes as well as fibroblasts on spider silk fibres was proven. Macroscopically, a comparable wound closure could be seen in spider silk and in sham controls. In histological evaluation, a thicker epidermis was observed in spider silk treated wounds while collagen III/I expression ratio was comparable in both groups. As native spider silk has been described as highly biocompatible, it might represent an innovative alternative to common wound dressings.

Lyadov A.S., Maksimova Y.M., Ilyin S.O., Gorbacheva S.N., Parenago O.P., Antonov S.V.

Greases based on poly-α-olefin oils of various viscosities, thickened with diureas of various structures, were studied systematically. The influence of the base oil viscosity and/or thickener structure on the physicochemical properties of lubricants was demonstrated. Diurea thickeners differing in the length of the hydrocarbon substituent influence the properties of lubricants prepared with base oils of similar chemical nature but different viscosity in different fashion. The revealed trends were confirmed by rheological studies in a wide range of shear rates. Tribological measurements at loads in the interval 20–80 kgf demonstrated significant dependence of the antiwear properties of ureate lubricants based on low-viscosity poly-α-olefin oils on the thickener structure. This dependence becomes less pronounced in going to higher-molecular-mass oils.

Sultan M.T., Lee O.J., Kim S.H., Ju H.W., Park C.H.

Silk fibroin (SF), a natural bioproduct, has been extensively used in biological and biomedical fields including wound healing due to its robust biocompatibility, less immunogenic, non-toxic, non-carcinogenic, and biodegradable properties. SF in different morphologic forms, such as hydrogels, sponges, films, electrospun nanofiber mats, and hydrocolloid dressings, have been successfully used for therapeutic use as wound dressings to induce the healing process. SF has also been known to promote wound healing by increasing the cell growth, proliferation, and migration of different cells types involved in the different phase of wound healing process. In this review, we summarize the different morphologic forms of SF that have been used in the treatment of various wound healing process. We also discuss the effect of SF on various cells types during the SF-induced healing process. Furthermore, we highlight molecular signaling aspects of the SF-induced healing process.