Cell Calcium, volume 71, pages 1-14

Coupling of P2Y receptors to Ca2+ mobilization in mesenchymal stromal cells from the human adipose tissue

Kotova Polina D ¹

Bystrova Marina F. ¹

Rogachevskaja Olga A ¹

Khokhlov Alexandr ¹

Sysoeva Veronika ²

Tkachuk Vsevolod A. ²

Kolesnikov Stanislav S ¹

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Institute of Cell Biophysics, Russian Academy of Sciences, Institutional Street 3, Pushchino, Moscow Region, 142290, Russia. |

Department of Biochemistry and Molecular Medicine, Faculty of Basic Medicine, Lomonosov Moscow State University, Russia. |

Publication type: Journal Article

Publication date: 2018-05-01

Elsevier

Journal: Cell Calcium

Quartile SCImago

Quartile WOS

Impact factor: 4

ISSN: 01434160, 15321991

DOI: 10.1016/j.ceca.2017.11.001

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Molecular Biology

Cell Biology

Physiology

Abstract

The purinergic transduction was examined in mesenchymal stromal cells (MSCs) from the human adipose tissue, and several nucleotides, including ATP, UTP, and ADP, were found to mobilize cytosolic Ca2+. Transcripts for multiple purinoreceptors were detected in MSC preparations, including A1, A2A, A2B, P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y13, P2Y14, P2X2, P2X4, and P2X7. Cellular responses to nucleotides were insignificantly sensitive to bath Ca2+, pointing at a minor contribution of Ca2+ entry, and were suppressed by U73122 and 2-APB, implicating the phosphoinositide cascade in coupling P2Y receptors to Ca2+ release. While individual cells were sensitive to several P2Y agonists, responsiveness to a given nucleotide varied from cell to cell, suggesting that particular MSCs could employ different sets of purinoreceptors. Caged Ca2+ stimulated Ca2+-induced Ca2+ release (CICR) that was mediated largely by IP3 receptors, and resultant Ca2+ transients were similar to nucleotide responses by magnitude and kinetics. A variety of findings hinted at CICR to be a universal mechanism that finalizes Ca2+ signaling initiated by agonists in MSCs. Individual MSCs responded to nucleotides in an all-or-nothing manner. Presumably just CICR provided invariant Ca2+ responses observed in MSCs at different nucleotide concentrations. The effects of isoform specific agonists and antagonists suggested that both P2Y1 and P2Y13 were obligatory for ADP responses, while P2Y4 and P2Y11 served as primary UTP and ATP receptors, respectively. Extracellular NAD+ stimulated Ca2+ signaling in each ATP-responsive MSC by involving P2Y11. The overall data indicate that extracellular nucleotides and NAD+ can serve as autocrine/paracrine factors regulating MSC functions.

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Citations by journals

	1 2 3
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology	Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 3, 15% Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology 3 publications, 15%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 10% International Journal of Molecular Sciences 2 publications, 10%
Frontiers in Cell and Developmental Biology	Frontiers in Cell and Developmental Biology, 2, 10% Frontiers in Cell and Developmental Biology 2 publications, 10%
European Journal of Pharmacology	European Journal of Pharmacology, 2, 10% European Journal of Pharmacology 2 publications, 10%
Cells	Cells, 1, 5% Cells 1 publication, 5%
Stem Cell Reviews and Reports	Stem Cell Reviews and Reports, 1, 5% Stem Cell Reviews and Reports 1 publication, 5%
Cellular and Molecular Life Sciences	Cellular and Molecular Life Sciences, 1, 5% Cellular and Molecular Life Sciences 1 publication, 5%
Purinergic Signalling	Purinergic Signalling, 1, 5% Purinergic Signalling 1 publication, 5%
Cell Calcium	Cell Calcium, 1, 5% Cell Calcium 1 publication, 5%
BBA Advances	BBA Advances, 1, 5% BBA Advances 1 publication, 5%
RSC Medicinal Chemistry	RSC Medicinal Chemistry, 1, 5% RSC Medicinal Chemistry 1 publication, 5%
Channels	Channels, 1, 5% Channels 1 publication, 5%
Function	Function, 1, 5% Function 1 publication, 5%
Calcium and Signal Transduction	Calcium and Signal Transduction, 1, 5% Calcium and Signal Transduction 1 publication, 5%
Experimental and Therapeutic Medicine	Experimental and Therapeutic Medicine, 1, 5% Experimental and Therapeutic Medicine 1 publication, 5%
	1 2 3

Citations by publishers

	1 2 3 4
Elsevier	Elsevier, 4, 20% Elsevier 4 publications, 20%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 3, 15% Multidisciplinary Digital Publishing Institute (MDPI) 3 publications, 15%
Springer Nature	Springer Nature, 3, 15% Springer Nature 3 publications, 15%
Pleiades Publishing	Pleiades Publishing, 3, 15% Pleiades Publishing 3 publications, 15%
Frontiers Media S.A.	Frontiers Media S.A., 2, 10% Frontiers Media S.A. 2 publications, 10%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 5% Royal Society of Chemistry (RSC) 1 publication, 5%
Taylor & Francis	Taylor & Francis, 1, 5% Taylor & Francis 1 publication, 5%
Oxford University Press	Oxford University Press, 1, 5% Oxford University Press 1 publication, 5%
InTech	InTech, 1, 5% InTech 1 publication, 5%
Spandidos Publications	Spandidos Publications, 1, 5% Spandidos Publications 1 publication, 5%
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Kotova P. D. et al. Coupling of P2Y receptors to Ca2+ mobilization in mesenchymal stromal cells from the human adipose tissue // Cell Calcium. 2018. Vol. 71. pp. 1-14.

GOST all authors (up to 50) Copy

Kotova P. D., Bystrova M. F., Rogachevskaja O. A., Khokhlov A., Sysoeva V., Tkachuk V. A., Kolesnikov S. S. Coupling of P2Y receptors to Ca2+ mobilization in mesenchymal stromal cells from the human adipose tissue // Cell Calcium. 2018. Vol. 71. pp. 1-14.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.ceca.2017.11.001

UR - https://doi.org/10.1016%2Fj.ceca.2017.11.001

TI - Coupling of P2Y receptors to Ca2+ mobilization in mesenchymal stromal cells from the human adipose tissue

T2 - Cell Calcium

AU - Kotova, Polina D

AU - Bystrova, Marina F.

AU - Rogachevskaja, Olga A

AU - Khokhlov, Alexandr

AU - Sysoeva, Veronika

AU - Tkachuk, Vsevolod A.

AU - Kolesnikov, Stanislav S

PY - 2018

DA - 2018/05/01 00:00:00

PB - Elsevier

SP - 1-14

VL - 71

SN - 0143-4160

SN - 1532-1991

ER -

BibTex

Cite this

BibTex Copy

@article{2018_Kotova,

author = {Polina D Kotova and Marina F. Bystrova and Olga A Rogachevskaja and Alexandr Khokhlov and Veronika Sysoeva and Vsevolod A. Tkachuk and Stanislav S Kolesnikov},

title = {Coupling of P2Y receptors to Ca2+ mobilization in mesenchymal stromal cells from the human adipose tissue},

journal = {Cell Calcium},

year = {2018},

volume = {71},

publisher = {Elsevier},

month = {may},

url = {https://doi.org/10.1016%2Fj.ceca.2017.11.001},

pages = {1--14},

doi = {10.1016/j.ceca.2017.11.001}

}

Found error?

Publisher

Elsevier

Journal

Cell Calcium

Quartile SCImago

Quartile WOS

Impact factor

ISSN

01434160 (Print)
15321991 (Electronic)

Labs

Laboratory of Molecular Cell Physiology

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