Open Access

Cell Reports, volume 20, issue 3, pages 521-528

Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1

Lomakin Ivan B. ¹

Stolboushkina Elena A ^{2, 3}

Vaidya Anand T ¹

Zhao Chenguang ¹

GARBER Maria B. ⁴

Dmitriev Sergey E. ^{2, 5}

Steitz Thomas A. ^{6, 7}

Hide authors affiliations Show authors affiliations: 7 affiliations

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520‐8114, USA |

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia |

Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia |

⁴

Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. |

⁵

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119234, Russia |

⁶

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520–8114, USA. |

⁷

Howard Hughes Medical Institute, Yale University, New Haven, CT 06520–8114, USA. |

Publication type: Journal Article

Publication date: 2017-07-18

Elsevier

Journal: Cell Reports

Quartile SCImago

Quartile WOS

Impact factor: 8.8

ISSN: 22111247

DOI: 10.1016/j.celrep.2017.06.025

Copy DOI

PubMed ID: 28723557

General Biochemistry, Genetics and Molecular Biology

Abstract

The repertoire of the density-regulated protein (DENR) and the malignant T cell-amplified sequence 1 (MCT-1/MCTS1) oncoprotein was recently expanded to include translational control of a specific set of cancer-related mRNAs. DENR and MCT-1 form the heterodimer, which binds to the ribosome and operates at both translation initiation and reinitiation steps, though by a mechanism that is yet unclear. Here, we determined the crystal structure of the human small ribosomal subunit in complex with DENR-MCT-1. The structure reveals the location of the DENR-MCT-1 dimer bound to the small ribosomal subunit. The binding site of the C-terminal domain of DENR on the ribosome has a striking similarity with those of canonical initiation factor 1 (eIF1), which controls the fidelity of translation initiation and scanning. Our findings elucidate how the DENR-MCT-1 dimer interacts with the ribosome and have functional implications for the mechanism of unconventional translation initiation and reinitiation.

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Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 2, 4.44% Proceedings of the National Academy of Sciences of the United States of America 2 publications, 4.44%
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Cell Death and Disease	Cell Death and Disease, 1, 2.22% Cell Death and Disease 1 publication, 2.22%
Molecular Cancer	Molecular Cancer, 1, 2.22% Molecular Cancer 1 publication, 2.22%
Nature	Nature, 1, 2.22% Nature 1 publication, 2.22%
Journal of Biological Chemistry	Journal of Biological Chemistry, 1, 2.22% Journal of Biological Chemistry 1 publication, 2.22%
PLoS Genetics	PLoS Genetics, 1, 2.22% PLoS Genetics 1 publication, 2.22%
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Computational and Structural Biotechnology Journal	Computational and Structural Biotechnology Journal, 1, 2.22% Computational and Structural Biotechnology Journal 1 publication, 2.22%
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FEMS Microbiology Reviews	FEMS Microbiology Reviews, 1, 2.22% FEMS Microbiology Reviews 1 publication, 2.22%
Biological Chemistry	Biological Chemistry, 1, 2.22% Biological Chemistry 1 publication, 2.22%
RNA	RNA, 1, 2.22% RNA 1 publication, 2.22%
Nature Chemical Biology	Nature Chemical Biology, 1, 2.22% Nature Chemical Biology 1 publication, 2.22%
G3: Genes, Genomes, Genetics	G3: Genes, Genomes, Genetics, 1, 2.22% G3: Genes, Genomes, Genetics 1 publication, 2.22%
Journal of Cell Science	Journal of Cell Science, 1, 2.22% Journal of Cell Science 1 publication, 2.22%

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Citations by publishers

	1 2 3 4 5 6 7 8 9
Elsevier	Elsevier, 9, 20% Elsevier 9 publications, 20%
Springer Nature	Springer Nature, 8, 17.78% Springer Nature 8 publications, 17.78%
Wiley	Wiley, 4, 8.89% Wiley 4 publications, 8.89%
Oxford University Press	Oxford University Press, 4, 8.89% Oxford University Press 4 publications, 8.89%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 3, 6.67% Cold Spring Harbor Laboratory 3 publications, 6.67%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 2, 4.44% Multidisciplinary Digital Publishing Institute (MDPI) 2 publications, 4.44%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 2, 4.44% Public Library of Science (PLoS) 2 publications, 4.44%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 2, 4.44% Proceedings of the National Academy of Sciences (PNAS) 2 publications, 4.44%
American Society for Biochemistry and Molecular Biology	American Society for Biochemistry and Molecular Biology, 1, 2.22% American Society for Biochemistry and Molecular Biology 1 publication, 2.22%
Dove Medical Press	Dove Medical Press, 1, 2.22% Dove Medical Press 1 publication, 2.22%
Walter de Gruyter	Walter de Gruyter, 1, 2.22% Walter de Gruyter 1 publication, 2.22%
Genetics Society of America	Genetics Society of America, 1, 2.22% Genetics Society of America 1 publication, 2.22%
The Company of Biologists	The Company of Biologists, 1, 2.22% The Company of Biologists 1 publication, 2.22%
	1 2 3 4 5 6 7 8 9

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GOST Copy

Lomakin I. B. et al. Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1 // Cell Reports. 2017. Vol. 20. No. 3. pp. 521-528.

GOST all authors (up to 50) Copy

Lomakin I. B., Stolboushkina E. A., Vaidya A. T., Zhao C., GARBER M. B., Dmitriev S. E., Steitz T. A. Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1 // Cell Reports. 2017. Vol. 20. No. 3. pp. 521-528.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1016/j.celrep.2017.06.025

UR - https://doi.org/10.1016%2Fj.celrep.2017.06.025

TI - Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1

T2 - Cell Reports

AU - Stolboushkina, Elena A

AU - Vaidya, Anand T

AU - GARBER, Maria B.

AU - Lomakin, Ivan B.

AU - Zhao, Chenguang

AU - Dmitriev, Sergey E.

AU - Steitz, Thomas A.

PY - 2017

DA - 2017/07/18 00:00:00

PB - Elsevier

SP - 521-528

IS - 3

VL - 20

PMID - 28723557

SN - 2211-1247

ER -

BibTex |

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BibTex Copy

@article{2017_Lomakin,

author = {Elena A Stolboushkina and Anand T Vaidya and Maria B. GARBER and Ivan B. Lomakin and Chenguang Zhao and Sergey E. Dmitriev and Thomas A. Steitz},

title = {Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1},

journal = {Cell Reports},

year = {2017},

volume = {20},

publisher = {Elsevier},

month = {jul},

url = {https://doi.org/10.1016%2Fj.celrep.2017.06.025},

number = {3},

pages = {521--528},

doi = {10.1016/j.celrep.2017.06.025}

}

MLA

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MLA Copy

Lomakin, Ivan B., et al. “Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1.” Cell Reports, vol. 20, no. 3, Jul. 2017, pp. 521-528. https://doi.org/10.1016%2Fj.celrep.2017.06.025.

Found error?

Publisher

Elsevier

Journal

Cell Reports

Quartile SCImago

Quartile WOS

Impact factor

8.8

ISSN

22111247 (Print)

Labs

Laboratory of structural studies of the broadcasting apparatus

Profiles

Stolboushkina, Elena Stolboushkina