Open Access

Cell Reports

, volume 37 , issue 12 , pages 110143

SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity

M. Gordon Joyce ^{1, 2}

Wei-Hung Chen ^{1, 3}

Rajeshwer S. Sankhala ^{1, 3}

Agnes Hajduczki ^{1, 3}

Paul Thomas ^{1, 3}

Misook Choe ^{1, 3}

Elizabeth J. Martinez ^{1, 3}

William C Chang ^{1, 3}

Caroline E. Peterson ^{1, 3}

Elaine B Morrison ⁴

Clayton S. Smith ^{5, 6}

Rita P.-Y. Chen ^{7, 8, 9, 10}

Aslaa Ahmed ¹¹

Lindsay Wieczorek ^{4, 12}

A S Anderson ^{13, 14}

James Brett Case ^{7, 8, 9, 10}

李艺凡 Li Yifan ^{4, 12}

Therese Oertel ^{13, 14}

Lorean Rosado ^{4, 12}

Akshaya Ganesh ^{13, 14}

Connor Whalen ^{13, 14}

Joshua M Carmen ⁴

Letzibeth Mendez Rivera ^{4, 12}

Christopher P Karch ^{4, 12}

Neelakshi Gohain ^{4, 12}

Zuzana Villar ^{4, 12}

David McCurdy ^{4, 12}

Zoltan Beck ^{4, 12}

Jiae Kim ^{4, 12}

Shikha Shrivastava ^{4, 12}

Ousman Jobe ^{4, 12}

Vincent Dussupt ^{4, 12}

Sebastian Molnar ^{4, 12}

Ursula Tran ^{4, 12}

Chandrika B Kannadka ^{4, 12}

Sandrine Soman ¹¹

Caitlin Kuklis ¹¹

Michelle Zemil ^{4, 12}

Htet Khanh ^{5, 6}

Wei-Min Wu ^{5, 6}

Matthew Cole ¹⁵

Debra K. Duso ¹⁵

Larry W. Kummer ¹⁵

Tricia J Lang ¹⁵

Shania E Muncil ¹⁵

Jeffrey R. Currier ¹¹

Shelly J. Krebs ^{4, 12}

Victoria R Polonis ⁴

Saravanan Rajan ¹⁶

Patrick M Mctamney ¹⁷

Mark T Esser ¹⁷

William Reiley ¹⁵

Morgane Rolland ^{4, 12}

Natalia de Val ^{5, 6}

MICHAEL P. DIAMOND ^{7, 8, 9, 10}

Gregory D. Gromowski ¹¹

Gary R Matyas ⁴

Mangala Rao ⁴

Nelson Michael ¹⁸

Kayvon Modjarrad ¹⁹

Hide authors affiliations Show authors affiliations: 19 affiliations

Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA |

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. Electronic address: gjoyce@eidresearch.org. |

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. |

⁴

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA. |

⁵

Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA |

⁶

Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Frederick, MD, USA. |

⁷

Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA |

⁸

Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA |

⁹

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA |

¹⁰

The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA. |

¹¹

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA. |

¹²

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA |

¹³

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA |

¹⁴

Oak Ridge Institute of Science and Education, Oak Ridge, TN, USA. |

¹⁵

Trudeau Institute, Inc., Saranac Lake, NY, USA. |

¹⁶

Antibody Discovery and Protein Engineering (ADPE), BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, USA. |

¹⁷

Microbial Sciences, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, USA. |

¹⁸

Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA. |

¹⁹

Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA. Electronic address: kayvon.modjarrad.civ@mail.mil. |

Publication type: Journal Article

Publication date: 2021-12-08

Elsevier

Cell Reports

scimago Q1

wos Q1

SJR: 3.796

CiteScore: 12.9

Impact factor: 6.9

ISSN: 22111247, 26391856

DOI: 10.1016/j.celrep.2021.110143

Copy DOI

PubMed ID: 34919799

General Biochemistry, Genetics and Molecular Biology

Abstract

Summary

The need for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) next-generation vaccines has been highlighted by the rise of variants of concern (VoCs) and the long-term threat of emerging coronaviruses. Here, we design and characterize four categories of engineered nanoparticle immunogens that recapitulate the structural and antigenic properties of the prefusion SARS-CoV-2 spike (S), S1, and receptor-binding domain (RBD). These immunogens induce robust S binding, ACE2 inhibition, and authentic and pseudovirus neutralizing antibodies against SARS-CoV-2. A spike-ferritin nanoparticle (SpFN) vaccine elicits neutralizing titers (ID₅₀ > 10,000) following a single immunization, whereas RBD-ferritin nanoparticle (RFN) immunogens elicit similar responses after two immunizations and also show durable and potent neutralization against circulating VoCs. Passive transfer of immunoglobulin G (IgG) purified from SpFN- or RFN-immunized mice protects K18-hACE2 transgenic mice from a lethal SARS-CoV-2 challenge. Furthermore, S-domain nanoparticle immunization elicits ACE2-blocking activity and ID₅₀ neutralizing antibody titers >2,000 against SARS-CoV-1, highlighting the broad response elicited by these immunogens.

Found

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Mikhailov Anatolii

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Moscow Institute of Physics and Technology

1 citation

Gushchin Ivan

🥼 🤝

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h-index: 25

Moscow Institute of Physics and Technology

The structure of proteins

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Ryzhykau Yury

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h-index: 12

Moscow Institute of Physics and Technology

Joint Institute for Nuclear Research

1 citation

Vlasov Alexey

🥼 🤝

PhD in Physics and Mathematics

35 publications, 401 citations

h-index: 13

Moscow Institute of Physics and Technology

Research interests

Biophysics

Membrane proteins

Protein crystallization

Small-angle X-ray scattering (Small-angle X-ray scattering)

Structural Biology

1 citation

Hossain M.

🥼 🤝

PhD in Engineering

282 publications, 12 086 citations, 628 reviews

h-index: 59

Bangladesh Atomic Energy Commission

1 citation

Shihab Uddin

🥼 🤝

PhD in Biological/biomedical sciences, senior lecturer, associate member of the Bangladesh Academy of Sciences

28 publications, 524 citations, 3 reviews

h-index: 12

King Fahd University of Petroleum and Minerals

Massachusetts Institute of Technology

Kyushu University

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Joyce M. G. et al. SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity // Cell Reports. 2021. Vol. 37. No. 12. p. 110143.

GOST all authors (up to 50) Copy

Joyce M. G. et al. SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity // Cell Reports. 2021. Vol. 37. No. 12. p. 110143.

RIS |

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BibTex (up to 50 authors) Copy

@article{2021_Joyce,

author = {M. Gordon Joyce and Wei-Hung Chen and Rajeshwer S. Sankhala and Agnes Hajduczki and Paul Thomas and Misook Choe and Elizabeth J. Martinez and William C Chang and Caroline E. Peterson and Elaine B Morrison and Clayton S. Smith and Rita P.-Y. Chen and Aslaa Ahmed and Lindsay Wieczorek and A S Anderson and James Brett Case and 李艺凡 Li Yifan and Therese Oertel and Lorean Rosado and Akshaya Ganesh and Connor Whalen and Joshua M Carmen and Letzibeth Mendez Rivera and Christopher P Karch and Neelakshi Gohain and Zuzana Villar and David McCurdy and Zoltan Beck and Jiae Kim and Shikha Shrivastava and Ousman Jobe and Vincent Dussupt and Sebastian Molnar and Ursula Tran and Chandrika B Kannadka and Sandrine Soman and Caitlin Kuklis and Michelle Zemil and Htet Khanh and Wei-Min Wu and Matthew Cole and Debra K. Duso and Larry W. Kummer and Tricia J Lang and Shania E Muncil and Jeffrey R. Currier and Shelly J. Krebs and Victoria R Polonis and Saravanan Rajan and Patrick M Mctamney and others},

title = {SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity},

journal = {Cell Reports},

year = {2021},

volume = {37},

publisher = {Elsevier},

month = {dec},

url = {https://doi.org/10.1016/j.celrep.2021.110143},

number = {12},

pages = {110143},

doi = {10.1016/j.celrep.2021.110143}

}

MLA

Cite this

MLA Copy

Joyce, M. Gordon, et al. “SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity.” Cell Reports, vol. 37, no. 12, Dec. 2021, p. 110143. https://doi.org/10.1016/j.celrep.2021.110143.

Publisher

Elsevier

Journal

Cell Reports

scimago Q1

wos Q1

SJR

3.796

CiteScore

12.9

Impact factor

6.9

ISSN

22111247 (Print)

26391856 (Print)

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