Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags
Roman S Erdmann
1
,
Stephanie Wood Baguley
2
,
Jennifer H. Richens
3
,
Rebecca F Wissner
1
,
Zhiqun Xi
2
,
Edward S. Allgeyer
3
,
Sheng Zhong
2
,
Alexander D Thompson
1
,
NICHOLAS LOWE
3
,
Richard Butler
3
,
Jan P. Bewersdorf
4
,
James E. Rothman
2
,
Daniel St. Johnston
3
,
Alanna Schepartz
1
,
Derek K. Toomre
2
Publication type: Journal Article
Publication date: 2019-04-01
scimago Q1
wos Q1
SJR: 2.659
CiteScore: 12.6
Impact factor: 7.2
ISSN: 24519448, 24519456
PubMed ID:
30745239
Drug Discovery
Biochemistry
Molecular Biology
Pharmacology
Clinical Biochemistry
Molecular Medicine
Abstract
Super-resolution microscopy requires that subcellular structures are labeled with bright and photostable fluorophores, especially for live-cell imaging. Organic fluorophores may help here as they can yield more photons-by orders of magnitude-than fluorescent proteins. To achieve molecular specificity with organic fluorophores in live cells, self-labeling proteins are often used, with HaloTags and SNAP-tags being the most common. However, how these two different tagging systems compare with each other is unclear, especially for stimulated emission depletion (STED) microscopy, which is limited to a small repertoire of fluorophores in living cells. Herein, we compare the two labeling approaches in confocal and STED imaging using various proteins and two model systems. Strikingly, we find that the fluorescent signal can be up to 9-fold higher with HaloTags than with SNAP-tags when using far-red rhodamine derivatives. This result demonstrates that the labeling strategy matters and can greatly influence the duration of super-resolution imaging.
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Total citations:
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Citations from 2024:
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(31%)
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GOST
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Erdmann R. S. et al. Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags // Cell Chemical Biology. 2019. Vol. 26. No. 4. pp. 584-592000000.
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Erdmann R. S., Baguley S. W., Richens J. H., Wissner R. F., Xi Z., Allgeyer E. S., Zhong S., Thompson A. D., LOWE N., Butler R., Bewersdorf J. P., Rothman J. E., St. Johnston D., Schepartz A., Toomre D. K. Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags // Cell Chemical Biology. 2019. Vol. 26. No. 4. pp. 584-592000000.
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TY - JOUR
DO - 10.1016/j.chembiol.2019.01.003
UR - https://doi.org/10.1016/j.chembiol.2019.01.003
TI - Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags
T2 - Cell Chemical Biology
AU - Erdmann, Roman S
AU - Baguley, Stephanie Wood
AU - Richens, Jennifer H.
AU - Wissner, Rebecca F
AU - Xi, Zhiqun
AU - Allgeyer, Edward S.
AU - Zhong, Sheng
AU - Thompson, Alexander D
AU - LOWE, NICHOLAS
AU - Butler, Richard
AU - Bewersdorf, Jan P.
AU - Rothman, James E.
AU - St. Johnston, Daniel
AU - Schepartz, Alanna
AU - Toomre, Derek K.
PY - 2019
DA - 2019/04/01
PB - Elsevier
SP - 584-592000000
IS - 4
VL - 26
PMID - 30745239
SN - 2451-9448
SN - 2451-9456
ER -
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BibTex (up to 50 authors)
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@article{2019_Erdmann,
author = {Roman S Erdmann and Stephanie Wood Baguley and Jennifer H. Richens and Rebecca F Wissner and Zhiqun Xi and Edward S. Allgeyer and Sheng Zhong and Alexander D Thompson and NICHOLAS LOWE and Richard Butler and Jan P. Bewersdorf and James E. Rothman and Daniel St. Johnston and Alanna Schepartz and Derek K. Toomre},
title = {Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags},
journal = {Cell Chemical Biology},
year = {2019},
volume = {26},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016/j.chembiol.2019.01.003},
number = {4},
pages = {584--592000000},
doi = {10.1016/j.chembiol.2019.01.003}
}
Cite this
MLA
Copy
Erdmann, Roman S., et al. “Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags.” Cell Chemical Biology, vol. 26, no. 4, Apr. 2019, pp. 584-592000000. https://doi.org/10.1016/j.chembiol.2019.01.003.