Experimental Eye Research, volume 125, pages 71-78

Metabolomics of the rat lens: A combined LC-MS and NMR study

Yanshole Vadim V ^{1, 2}

Snytnikova Olga A ³

Kiryutin Alexey S. ³

Yanshole Lyudmila V ³

Sagdeev Renad Z ³

Tsentalovich Yuri P ⁴

Hide authors affiliations Show authors affiliations: 4 affiliations

International Tomography Center SB RAS, Institutskaya 3A, Novosibirsk, 630090, Russia |

Novosibirsk State University, Pirogova 2, Novosibirsk, 630090, Russia. |

International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia. |

⁴

Novosibirsk State University , Pirogova 2 , Novosibirsk 630090 , Russia. |

Publication type: Journal Article

Publication date: 2014-08-01

Elsevier

Journal: Experimental Eye Research

Quartile SCImago

Quartile WOS

Impact factor: 3.4

ISSN: 00144835, 10960007

DOI: 10.1016/j.exer.2014.05.016

Copy DOI

Cellular and Molecular Neuroscience

Ophthalmology

Sensory Systems

Abstract

This work is the first comprehensive report on the quantitative metabolomic composition of the rat lens. Quantitative metabolomic profiles of lenses were acquired with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. More than forty low molecular weight compounds found in the lens have been reliably identified and quantified. The most abundant metabolites in the 3-month-old Wistar rat lens are taurine, hypotaurine , lactate, phosphocholine and reduced glutathione . The analysis of age-related changes in the lens metabolomic composition shows a gradual decrease of the content of most metabolites. This decrease is the most pronounced between 1 and 3 months, which probably corresponds to the completion of the lens maturation in one-month-old rats and to the high rate of the young lens growth. The enhanced levels of tryptophan , tyrosine , carnitine , glycerophosphate , GSH and GSSG were found in lenses of senescence-accelerated OXYS rats ; for some metabolites, this effect may probably be attributed to the compensatory response to oxidative stress . • Lens metabolomic profiling is performed by combination of NMR and LC-MS methods. • 42 metabolites found in the rat lens are identified and quantified. • The content of most metabolites decreases with age. • The level of some metabolites is elevated in lenses of senescence-accelerated rats.

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Citations by journals

	1 2 3 4 5
Metabolites	Metabolites, 5, 10.2% Metabolites 5 publications, 10.2%
Metabolomics	Metabolomics, 5, 10.2% Metabolomics 5 publications, 10.2%
Experimental Eye Research	Experimental Eye Research, 5, 10.2% Experimental Eye Research 5 publications, 10.2%
Scientific Reports	Scientific Reports, 3, 6.12% Scientific Reports 3 publications, 6.12%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 3, 6.12% International Journal of Molecular Sciences 3 publications, 6.12%
PLoS ONE	PLoS ONE, 2, 4.08% PLoS ONE 2 publications, 4.08%
F1000Research	F1000Research, 1, 2.04% F1000Research 1 publication, 2.04%
Journal of International Medical Research	Journal of International Medical Research, 1, 2.04% Journal of International Medical Research 1 publication, 2.04%
Nutrients	Nutrients, 1, 2.04% Nutrients 1 publication, 2.04%
Molecules	Molecules, 1, 2.04% Molecules 1 publication, 2.04%
Biology	Biology, 1, 2.04% Biology 1 publication, 2.04%
Frontiers in Cell and Developmental Biology	Frontiers in Cell and Developmental Biology, 1, 2.04% Frontiers in Cell and Developmental Biology 1 publication, 2.04%
Biogerontology	Biogerontology, 1, 2.04% Biogerontology 1 publication, 2.04%
Stem Cell Reviews and Reports	Stem Cell Reviews and Reports, 1, 2.04% Stem Cell Reviews and Reports 1 publication, 2.04%
European Journal of Pharmacology	European Journal of Pharmacology, 1, 2.04% European Journal of Pharmacology 1 publication, 2.04%
Free Radical Biology and Medicine	Free Radical Biology and Medicine, 1, 2.04% Free Radical Biology and Medicine 1 publication, 2.04%
Journal of Photochemistry and Photobiology B: Biology	Journal of Photochemistry and Photobiology B: Biology, 1, 2.04% Journal of Photochemistry and Photobiology B: Biology 1 publication, 2.04%
Analytica Chimica Acta	Analytica Chimica Acta, 1, 2.04% Analytica Chimica Acta 1 publication, 2.04%
Neurobiology of Aging	Neurobiology of Aging, 1, 2.04% Neurobiology of Aging 1 publication, 2.04%
Journal of Mass Spectrometry	Journal of Mass Spectrometry, 1, 2.04% Journal of Mass Spectrometry 1 publication, 2.04%
Journal of Agricultural and Food Chemistry	Journal of Agricultural and Food Chemistry, 1, 2.04% Journal of Agricultural and Food Chemistry 1 publication, 2.04%
Physical Chemistry Chemical Physics	Physical Chemistry Chemical Physics, 1, 2.04% Physical Chemistry Chemical Physics 1 publication, 2.04%
Doklady Biochemistry and Biophysics	Doklady Biochemistry and Biophysics, 1, 2.04% Doklady Biochemistry and Biophysics 1 publication, 2.04%
Critical Reviews in Analytical Chemistry	Critical Reviews in Analytical Chemistry, 1, 2.04% Critical Reviews in Analytical Chemistry 1 publication, 2.04%
American Journal of Physiology - Gastrointestinal and Liver Physiology	American Journal of Physiology - Gastrointestinal and Liver Physiology, 1, 2.04% American Journal of Physiology - Gastrointestinal and Liver Physiology 1 publication, 2.04%
eLife	eLife, 1, 2.04% eLife 1 publication, 2.04%
Redox Biology	Redox Biology, 1, 2.04% Redox Biology 1 publication, 2.04%
Journal of Alzheimer's Disease	Journal of Alzheimer's Disease, 1, 2.04% Journal of Alzheimer's Disease 1 publication, 2.04%
Advances in Gerontology	Advances in Gerontology, 1, 2.04% Advances in Gerontology 1 publication, 2.04%

	1 2 3 4 5

Citations by publishers

	2 4 6 8 10 12
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 11, 22.45% Multidisciplinary Digital Publishing Institute (MDPI) 11 publications, 22.45%
Elsevier	Elsevier, 11, 22.45% Elsevier 11 publications, 22.45%
Springer Nature	Springer Nature, 10, 20.41% Springer Nature 10 publications, 20.41%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 2, 4.08% Public Library of Science (PLoS) 2 publications, 4.08%
Pleiades Publishing	Pleiades Publishing, 2, 4.08% Pleiades Publishing 2 publications, 4.08%
F1000 Research	F1000 Research, 1, 2.04% F1000 Research 1 publication, 2.04%
SAGE	SAGE, 1, 2.04% SAGE 1 publication, 2.04%
Frontiers Media S.A.	Frontiers Media S.A., 1, 2.04% Frontiers Media S.A. 1 publication, 2.04%
Wiley	Wiley, 1, 2.04% Wiley 1 publication, 2.04%
American Chemical Society (ACS)	American Chemical Society (ACS), 1, 2.04% American Chemical Society (ACS) 1 publication, 2.04%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 2.04% Royal Society of Chemistry (RSC) 1 publication, 2.04%
Taylor & Francis	Taylor & Francis, 1, 2.04% Taylor & Francis 1 publication, 2.04%
American Physiological Society	American Physiological Society, 1, 2.04% American Physiological Society 1 publication, 2.04%
eLife Sciences Publications	eLife Sciences Publications, 1, 2.04% eLife Sciences Publications 1 publication, 2.04%
IOS Press	IOS Press, 1, 2.04% IOS Press 1 publication, 2.04%
	2 4 6 8 10 12

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GOST Copy

Yanshole V. V. et al. Metabolomics of the rat lens: A combined LC-MS and NMR study // Experimental Eye Research. 2014. Vol. 125. pp. 71-78.

GOST all authors (up to 50) Copy

Yanshole V. V., Snytnikova O. A., Kiryutin A. S., Yanshole L. V., Sagdeev R. Z., Tsentalovich Y. P. Metabolomics of the rat lens: A combined LC-MS and NMR study // Experimental Eye Research. 2014. Vol. 125. pp. 71-78.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.exer.2014.05.016

UR - https://doi.org/10.1016%2Fj.exer.2014.05.016

TI - Metabolomics of the rat lens: A combined LC-MS and NMR study

T2 - Experimental Eye Research

AU - Yanshole, Vadim V

AU - Snytnikova, Olga A

AU - Kiryutin, Alexey S.

AU - Yanshole, Lyudmila V

AU - Sagdeev, Renad Z

AU - Tsentalovich, Yuri P

PY - 2014

DA - 2014/08/01 00:00:00

PB - Elsevier

SP - 71-78

VL - 125

SN - 0014-4835

SN - 1096-0007

ER -

BibTex

Cite this

BibTex Copy

@article{2014_Yanshole,

author = {Vadim V Yanshole and Olga A Snytnikova and Alexey S. Kiryutin and Lyudmila V Yanshole and Renad Z Sagdeev and Yuri P Tsentalovich},

title = {Metabolomics of the rat lens: A combined LC-MS and NMR study},

journal = {Experimental Eye Research},

year = {2014},

volume = {125},

publisher = {Elsevier},

month = {aug},

url = {https://doi.org/10.1016%2Fj.exer.2014.05.016},

pages = {71--78},

doi = {10.1016/j.exer.2014.05.016}

}

Found error?

Publisher

Elsevier

Journal

Experimental Eye Research

Quartile SCImago

Quartile WOS

Impact factor

3.4

ISSN

00144835 (Print)
10960007 (Electronic)

Labs

Laboratory of Proteomics and Metabolomics

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