Gene, volume 809, pages 146024
CRISPR-Cas9 mediated knockout of AnxA6 gene enhances influenza A virus replication in low-permissive HEK293FT cell line
Komissarov A., Sergeeva M., Zhuravlev E., Medvedev S., Malakhova A., Andreeva E., Shurygina A., Gorshkov A., Timofeeva M., Balakhonova E., Grudinin M., Zakian S., Richter V., Stepanov G.
Publication type: Journal Article
Publication date: 2022-01-01
General Medicine
Genetics
Abstract
Using cell cultures of human origin for the propagation of influenza virus is an attractive way to preserve its glycosylation profile and antigenic properties, which is essential in influenza surveillance and vaccine production. However, only few cell lines are highly permissive to influenza virus, and none of them are of human origin. The barrier might be associated with host restriction factors inhibiting influenza growth, such as AnxA6 protein counteracting the process of influenza virion packaging. In the presented work we explore the CRISPR-Cas9 mediated knockout of ANXA6 gene as a way to overcome the host restriction barrier and increase the susceptibility of human cell line to influenza infection. By CRISPR-Cas9 genome editing we modified HEK293FT cells and obtained several clones defective in the ANXA6 gene. The replication of the influenza A virus in original HEK293FT cells and the HEK293FT-ANXA6-/- mutant cells was compared in growth curve experiments. By combination of methods including TCID assay and flow cytometry we showed that accumulation of influenza A virus in the mutant HEK293FT-ANXA6-/- cells significantly exceeded the virus titer in the original HEK293FT cells.
Citations by journals
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Virus Research
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Virus Research
1 publication, 33.33%
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Pathogens
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Pathogens
1 publication, 33.33%
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Citations by publishers
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Elsevier
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Elsevier
1 publication, 33.33%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 33.33%
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1
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Komissarov A. et al. CRISPR-Cas9 mediated knockout of AnxA6 gene enhances influenza A virus replication in low-permissive HEK293FT cell line // Gene. 2022. Vol. 809. p. 146024.
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Komissarov A., Sergeeva M., Zhuravlev E., Medvedev S., Malakhova A., Andreeva E., Shurygina A., Gorshkov A., Timofeeva M., Balakhonova E., Grudinin M., Zakian S., Richter V., Stepanov G. CRISPR-Cas9 mediated knockout of AnxA6 gene enhances influenza A virus replication in low-permissive HEK293FT cell line // Gene. 2022. Vol. 809. p. 146024.
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TY - JOUR
DO - 10.1016/j.gene.2021.146024
UR - https://doi.org/10.1016%2Fj.gene.2021.146024
TI - CRISPR-Cas9 mediated knockout of AnxA6 gene enhances influenza A virus replication in low-permissive HEK293FT cell line
T2 - Gene
AU - Komissarov, A
AU - Sergeeva, M
AU - Zhuravlev, E
AU - Medvedev, S
AU - Malakhova, A
AU - Andreeva, E
AU - Shurygina, A
AU - Gorshkov, A
AU - Timofeeva, M
AU - Balakhonova, E
AU - Grudinin, M
AU - Zakian, S
AU - Richter, V
AU - Stepanov, G
PY - 2022
DA - 2022/01/01 00:00:00
PB - Elsevier
SP - 146024
VL - 809
SN - 0378-1119
SN - 1879-0038
ER -
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@article{2022,
author = {A Komissarov and M Sergeeva and E Zhuravlev and S Medvedev and A Malakhova and E Andreeva and A Shurygina and A Gorshkov and M Timofeeva and E Balakhonova and M Grudinin and S Zakian and V Richter and G Stepanov},
title = {CRISPR-Cas9 mediated knockout of AnxA6 gene enhances influenza A virus replication in low-permissive HEK293FT cell line},
journal = {Gene},
year = {2022},
volume = {809},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016%2Fj.gene.2021.146024},
pages = {146024},
doi = {10.1016/j.gene.2021.146024}
}