Industrial Crops and Products, volume 177, pages 114417
Predominance of oleanane-type ginsenoside R0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus C.A. Meyer
Glagoleva Elena S.
1
,
Konstantinova Svetlana V
1
,
Kochkin Dmitry V.
1
,
Ossipov Vladimir
2
,
Titova Maria V.
3
,
Popova Elena V.
3
,
Nosov Alexander M.
1
,
Paek Kee Yoeup
4
3
4
Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju 361-763, South Korea
|
Publication type: Journal Article
Publication date: 2022-03-01
Journal:
Industrial Crops and Products
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.9
ISSN: 09266690
Agronomy and Crop Science
Abstract
Cell culture of Panax spp . is a potent sustainable source of biologically active triterpene glycosides (ginsenosides) specific for this genus. In this study, growth and biosynthetic profiles were investigated for P. japonicus suspension cell culture maintained for over 20 years by periodic subcultures in flasks. Cell culture demonstrated intensive growth evidenced by high accumulation of dry weight (DW, 9.8 gL −1 ), viability (80–90%) and specific growth rate (0.12 day −1 ) that were comparable to those recorded at the year of culture induction. Nineteen structurally different ginsenosides were identified in cell biomass using UPLC-Q-Exactive-Orbitrap HRMS and HPLC-ESI-Q-MS. Ginsenoside R 0 and malonyl-ginsenosides (MalGs) accounted for over 80% of the total ginsenoside content in cells during the subculture cycle while the sum of neutral ginsenosides of protopanaxatriol (PPT: Re, Rg 1 , Rf) and protopanaxadiol (PPD: Rb 1 , Rb 2 , Rc, Rd) groups constituted less than 20%. Ginsenoside contents were maximized during growth decline phase as 35.3 mg/gDW for R 0 , 48.7 mg/gDW for MalGs, 10.4 mg/gDW for PPD and 5.2 mg/gDW for PPT. These data confirmed that 20-year-old cell culture of P. japonicus retained the ability for intensive growth and accumulation of wide spectrum of ginsenosides which opens the door for wide application of this culture. • 20-year-old suspension cell culture of Panax japonicus shows stable growth and biosynthetic characteristics. • UPLC-Q-Exactive-Orbitrap HRMS was used for metabolite identification. • Cell culture accumulates 19 structurally diverse ginsenosides. • Major compounds in cell culture are malonyl-ginsenosides (47.8 mg/g dry weight) and R 0 (35.3 mg/g dry weight).
Citations by journals
|
1
|
|
|
Nutrients
|
Nutrients
1 publication, 11.11%
|
|
Agronomy
|
Agronomy
1 publication, 11.11%
|
|
Biology
|
Biology
1 publication, 11.11%
|
|
Drug development & registration
|
Drug development & registration
1 publication, 11.11%
|
|
Industrial Crops and Products
|
Industrial Crops and Products
1 publication, 11.11%
|
|
Plant Cell, Tissue and Organ Culture
|
Plant Cell, Tissue and Organ Culture
1 publication, 11.11%
|
|
Russian Journal of Plant Physiology
|
Russian Journal of Plant Physiology
1 publication, 11.11%
|
|
Plants
|
Plants
1 publication, 11.11%
|
|
Frontiers in Plant Science
|
Frontiers in Plant Science
1 publication, 11.11%
|
|
1
|
Citations by publishers
|
1
2
3
4
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
4 publications, 44.44%
|
|
Center of Pharmaceutical Analytics Ltd
|
Center of Pharmaceutical Analytics Ltd
1 publication, 11.11%
|
|
Elsevier
|
Elsevier
1 publication, 11.11%
|
|
Springer Nature
|
Springer Nature
1 publication, 11.11%
|
|
Pleiades Publishing
|
Pleiades Publishing
1 publication, 11.11%
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
1 publication, 11.11%
|
|
1
2
3
4
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2022,2023,2024],"ids":[0,0,0],"codes":[0,0,0],"imageUrls":["","",""],"datasets":[{"label":"Citations number","data":[1,4,4],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":["11.11","44.44","44.44"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Nutrients","Agronomy","Biology","Drug development & registration","Industrial Crops and Products","Plant Cell, Tissue and Organ Culture","Russian Journal of Plant Physiology","Plants","Frontiers in Plant Science"],"ids":[3372,8267,24144,26766,555,12513,13830,17251,1823],"codes":[0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/ewwj5yBvA6wToa4jkXRrcDjN2omUCp6UYltwKhS8_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[11.11,11.11,11.11,11.11,11.11,11.11,11.11,11.11,11.11],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Center of Pharmaceutical Analytics Ltd","Elsevier","Springer Nature","Pleiades Publishing","Frontiers Media S.A."],"ids":[202,7156,17,8,101,208],"codes":[0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/ewwj5yBvA6wToa4jkXRrcDjN2omUCp6UYltwKhS8_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp"],"datasets":[{"label":"","data":[4,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[44.44,11.11,11.11,11.11,11.11,11.11],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Glagoleva E. S. et al. Predominance of oleanane-type ginsenoside R0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus C.A. Meyer // Industrial Crops and Products. 2022. Vol. 177. p. 114417.
GOST all authors (up to 50)
Copy
Glagoleva E. S., Konstantinova S. V., Kochkin D. V., Ossipov V., Titova M. V., Popova E. V., Nosov A. M., Paek K. Y. Predominance of oleanane-type ginsenoside R0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus C.A. Meyer // Industrial Crops and Products. 2022. Vol. 177. p. 114417.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/j.indcrop.2021.114417
UR - https://doi.org/10.1016%2Fj.indcrop.2021.114417
TI - Predominance of oleanane-type ginsenoside R0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus C.A. Meyer
T2 - Industrial Crops and Products
AU - Glagoleva, Elena S.
AU - Konstantinova, Svetlana V
AU - Kochkin, Dmitry V.
AU - Titova, Maria V.
AU - Popova, Elena V.
AU - Nosov, Alexander M.
AU - Paek, Kee Yoeup
AU - Ossipov, Vladimir
PY - 2022
DA - 2022/03/01 00:00:00
PB - Elsevier
SP - 114417
VL - 177
SN - 0926-6690
ER -
Cite this
BibTex
Copy
@article{2022_Glagoleva,
author = {Elena S. Glagoleva and Svetlana V Konstantinova and Dmitry V. Kochkin and Maria V. Titova and Elena V. Popova and Alexander M. Nosov and Kee Yoeup Paek and Vladimir Ossipov},
title = {Predominance of oleanane-type ginsenoside R0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus C.A. Meyer},
journal = {Industrial Crops and Products},
year = {2022},
volume = {177},
publisher = {Elsevier},
month = {mar},
url = {https://doi.org/10.1016%2Fj.indcrop.2021.114417},
pages = {114417},
doi = {10.1016/j.indcrop.2021.114417}
}
Profiles