Journal of Molecular Biology, volume 425, issue 6, pages 989-998
Crystal Structure of the Archaeal Translation Initiation Factor 2 in Complex with a GTP Analogue and Met-tRNAfMet
Publication type: Journal Article
Publication date: 2013-03-01
Journal:
Journal of Molecular Biology
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.6
ISSN: 00222836, 10898638
Molecular Biology
Structural Biology
Abstract
Heterotrimeric aIF2αβγ (archaeal homologue of the eukaryotic translation initiation factor 2) in its GTP-bound form delivers Met-tRNAi(Met) to the small ribosomal subunit. It is known that the heterodimer containing the GTP-bound γ subunit and domain 3 of the α subunit of aIF2 is required for the formation of a stable complex with Met-tRNAi. Here, the crystal structure of an incomplete ternary complex including aIF2αD3γ⋅GDPNP⋅Met-tRNAf(Met) has been solved at 3.2Å resolution. This structure is in good agreement with biochemical and hydroxyl radical probing data. The analysis of the complex shows that despite the structural similarity of aIF2γ and the bacterial translation elongation factor EF-Tu, their modes of tRNA binding are very different. Remarkably, the recently published 5.0-Å-resolution structure of almost the same ternary initiation complex differs dramatically from the structure presented. Reasons for this discrepancy are discussed.
Citations by journals
|
1
2
|
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
2 publications, 15.38%
|
|
Scientific Reports
|
Scientific Reports
2 publications, 15.38%
|
|
Acta Crystallographica Section D Biological Crystallography
|
Acta Crystallographica Section D Biological Crystallography
1 publication, 7.69%
|
|
Nature Communications
|
Nature Communications
1 publication, 7.69%
|
|
Journal of Structural and Functional Genomics
|
Journal of Structural and Functional Genomics
1 publication, 7.69%
|
|
Journal of Molecular Biology
|
Journal of Molecular Biology
1 publication, 7.69%
|
|
ChemBioChem
|
ChemBioChem
1 publication, 7.69%
|
|
Biochemistry (Moscow)
|
Biochemistry (Moscow)
1 publication, 7.69%
|
|
Crystallography Reports
|
Crystallography Reports
1 publication, 7.69%
|
|
Nucleic Acids Research
|
Nucleic Acids Research
1 publication, 7.69%
|
|
Annual Review of Biophysics
|
Annual Review of Biophysics
1 publication, 7.69%
|
|
1
2
|
Citations by publishers
|
1
2
3
4
|
|
|
Springer Nature
|
Springer Nature
4 publications, 30.77%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 15.38%
|
|
Pleiades Publishing
|
Pleiades Publishing
2 publications, 15.38%
|
|
International Union of Crystallography (IUCr)
|
International Union of Crystallography (IUCr)
1 publication, 7.69%
|
|
Elsevier
|
Elsevier
1 publication, 7.69%
|
|
Wiley
|
Wiley
1 publication, 7.69%
|
|
Oxford University Press
|
Oxford University Press
1 publication, 7.69%
|
|
Annual Reviews
|
Annual Reviews
1 publication, 7.69%
|
|
1
2
3
4
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2013,2014,2015,2016,2017,2018,2019,2020,2021],"ids":[0,0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","","",""],"datasets":[{"label":"Citations number","data":[2,1,3,4,0,1,1,0,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["15.38","7.69","23.08","30.77",0,"7.69","7.69",0,"7.69"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["International Journal of Molecular Sciences","Scientific Reports","Acta Crystallographica Section D Biological Crystallography","Nature Communications","Journal of Structural and Functional Genomics","Journal of Molecular Biology","ChemBioChem","Biochemistry (Moscow)","Crystallography Reports","Nucleic Acids Research","Annual Review of Biophysics"],"ids":[14627,13767,25462,3231,25924,19701,12154,2119,24474,23904,15936],"codes":[0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/xG3oC19920mmFVqkoVEGKGaMCeoalzyvWxPkVox5_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp"],"datasets":[{"label":"","data":[2,2,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[15.38,15.38,7.69,7.69,7.69,7.69,7.69,7.69,7.69,7.69,7.69],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Springer Nature","Multidisciplinary Digital Publishing Institute (MDPI)","Pleiades Publishing","International Union of Crystallography (IUCr)","Elsevier","Wiley","Oxford University Press","Annual Reviews"],"ids":[8,202,101,730,17,11,19,6941],"codes":[0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/xG3oC19920mmFVqkoVEGKGaMCeoalzyvWxPkVox5_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp"],"datasets":[{"label":"","data":[4,2,2,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[30.77,15.38,15.38,7.69,7.69,7.69,7.69,7.69],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Stolboushkina E. et al. Crystal Structure of the Archaeal Translation Initiation Factor 2 in Complex with a GTP Analogue and Met-tRNAfMet // Journal of Molecular Biology. 2013. Vol. 425. No. 6. pp. 989-998.
GOST all authors (up to 50)
Copy
Stolboushkina E., Nikonov S., Zelinskaya N., Arkhipova V., Nikulin A. D., Garber M., Nikonov O. Crystal Structure of the Archaeal Translation Initiation Factor 2 in Complex with a GTP Analogue and Met-tRNAfMet // Journal of Molecular Biology. 2013. Vol. 425. No. 6. pp. 989-998.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/j.jmb.2012.12.023
UR - https://doi.org/10.1016%2Fj.jmb.2012.12.023
TI - Crystal Structure of the Archaeal Translation Initiation Factor 2 in Complex with a GTP Analogue and Met-tRNAfMet
T2 - Journal of Molecular Biology
AU - Stolboushkina, Elena
AU - Nikonov, Stanislav
AU - Zelinskaya, Natalia
AU - Arkhipova, Valentina
AU - Nikulin, Alexey D.
AU - Garber, Maria
AU - Nikonov, Oleg
PY - 2013
DA - 2013/03/01 00:00:00
PB - Elsevier
SP - 989-998
IS - 6
VL - 425
SN - 0022-2836
SN - 1089-8638
ER -
Cite this
BibTex
Copy
@article{2013_Stolboushkina,
author = {Elena Stolboushkina and Stanislav Nikonov and Natalia Zelinskaya and Valentina Arkhipova and Alexey D. Nikulin and Maria Garber and Oleg Nikonov},
title = {Crystal Structure of the Archaeal Translation Initiation Factor 2 in Complex with a GTP Analogue and Met-tRNAfMet},
journal = {Journal of Molecular Biology},
year = {2013},
volume = {425},
publisher = {Elsevier},
month = {mar},
url = {https://doi.org/10.1016%2Fj.jmb.2012.12.023},
number = {6},
pages = {989--998},
doi = {10.1016/j.jmb.2012.12.023}
}
Cite this
MLA
Copy
Stolboushkina, Elena, et al. “Crystal Structure of the Archaeal Translation Initiation Factor 2 in Complex with a GTP Analogue and Met-tRNAfMet.” Journal of Molecular Biology, vol. 425, no. 6, Mar. 2013, pp. 989-998. https://doi.org/10.1016%2Fj.jmb.2012.12.023.