Open Access
A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer
Publication type: Journal Article
Publication date: 2022-11-05
scimago Q1
wos Q1
SJR: 1.025
CiteScore: 11.0
Impact factor: 7.6
ISSN: 26660539
PubMed ID:
36373144
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering
Computer Science (miscellaneous)
Instrumentation
Abstract
Multiplex assays often rely on expensive sensors incorporating covalently linked fluorescent dyes. Herein, we developed a self-assembling aptamer-based multiplex assay. This multiplex approach utilizes a previously established split aptamer sensor in conjugation with a novel split aptamer sensor based upon a malachite green DNA aptamer. This system was capable of simultaneous fluorescent detection of two SARS COVID-19-related sequences in one sample with individual sensors that possesses a limit of detection (LOD) in the low nM range. Optimization of the Split Malachite Green (SMG) sensor yielded a minimized aptamer construct, Mini-MG, capable of inducing fluorescence of malachite green in both a DNA hairpin and sensor format.
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Metrics
8
Total citations:
8
Citations from 2024:
5
(62.5%)
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GOST
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R． O'Steen M., Kolpashchikov D. M. A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer // Sensors and Actuators Reports. 2022. Vol. 4. p. 100125.
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R． O'Steen M., Kolpashchikov D. M. A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer // Sensors and Actuators Reports. 2022. Vol. 4. p. 100125.
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RIS
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TY - JOUR
DO - 10.1016/j.snr.2022.100125
UR - https://doi.org/10.1016/j.snr.2022.100125
TI - A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer
T2 - Sensors and Actuators Reports
AU - R． O'Steen, Martin
AU - Kolpashchikov, Dmitry M.
PY - 2022
DA - 2022/11/05
PB - Elsevier
SP - 100125
VL - 4
PMID - 36373144
SN - 2666-0539
ER -
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BibTex (up to 50 authors)
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@article{2022_R． O'Steen,
author = {Martin R． O'Steen and Dmitry M. Kolpashchikov},
title = {A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer},
journal = {Sensors and Actuators Reports},
year = {2022},
volume = {4},
publisher = {Elsevier},
month = {nov},
url = {https://doi.org/10.1016/j.snr.2022.100125},
pages = {100125},
doi = {10.1016/j.snr.2022.100125}
}
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