Journal of Chromatography B Biomedical Sciences and Applications, volume 727, issue 1-2, pages 43-52
Analysis of diagnostic metabolites by capillary electrophoresis–mass spectrometry
1
Core Technologies Area, Discovery Research, Novartis Pharmaceuticals Corporation, 556 Morris Avenue, Summit, NJ 07901, USA
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Publication type: Journal Article
Publication date: 1999-04-01
Quartile SCImago
— Quartile WOS
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Impact factor: —
ISSN: 03784347
General Chemistry
Abstract
We describe here a procedure by capillary electrophoresis-mass spectrometry (CE-MS) for the direct analysis of urine samples on diagnostic metabolites, which are present in patient urine with metabolic disorders. The method was demonstrated using urine samples spiked with diagnostic metabolites, including glutathione for gamma-glutamyl transpeptidase deficiency, pyroglutamate for generalized glutathione deficiency, adenylosuccinate for adenylosuccinase deficiency, omithine for gyrate atrophy, histidine for histidinemia, and homogentisic acid for alcaptonuria, at concentrations similar to those found in patients' urine. A coaxial sheath liquid flow was used for coupling CE and MS in electrospray ionization mode. Identification of the metabolites is based on their molecular weights and fragmentation patterns. The CE-MS method is highly specific and sensitive comparing to the previously reported method using migration time and UV absorption for identification. It should find broad application in clinical and pharmaceutical research and development.
Top-30
Citations by journals
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12
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Electrophoresis
11 publications, 29.73%
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Journal of Chromatography A
7 publications, 18.92%
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Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
2 publications, 5.41%
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Chromatographia
1 publication, 2.7%
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Bulletin of the Korean Chemical Society
1 publication, 2.7%
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Talanta
1 publication, 2.7%
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Clinica Chimica Acta
1 publication, 2.7%
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TrAC - Trends in Analytical Chemistry
1 publication, 2.7%
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Rapid Communications in Mass Spectrometry
1 publication, 2.7%
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Biomedical Chromatography
1 publication, 2.7%
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Chinese Journal of Chemistry
1 publication, 2.7%
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Mass Spectrometry Reviews
1 publication, 2.7%
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Journal of Mass Spectrometry
1 publication, 2.7%
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Analytical Chemistry
1 publication, 2.7%
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Journal of Chromatography Library
1 publication, 2.7%
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Clinical Chemistry
1 publication, 2.7%
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Clinical Chemistry and Laboratory Medicine
1 publication, 2.7%
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Advances in Experimental Medicine and Biology
1 publication, 2.7%
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Russian Chemical Reviews
1 publication, 2.7%
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Citations by publishers
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Wiley
17 publications, 45.95%
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Elsevier
13 publications, 35.14%
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Springer Nature
2 publications, 5.41%
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American Chemical Society (ACS)
1 publication, 2.7%
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American Association for Clinical Chemistry
1 publication, 2.7%
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Walter de Gruyter
1 publication, 2.7%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 2.7%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Chen Y. et al. Analysis of diagnostic metabolites by capillary electrophoresis–mass spectrometry // Journal of Chromatography B Biomedical Sciences and Applications. 1999. Vol. 727. No. 1-2. pp. 43-52.
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Chen Y., Quinn D., Fu E., Wang Y. Analysis of diagnostic metabolites by capillary electrophoresis–mass spectrometry // Journal of Chromatography B Biomedical Sciences and Applications. 1999. Vol. 727. No. 1-2. pp. 43-52.
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TY - JOUR
DO - 10.1016/s0378-4347(99)00045-6
UR - https://doi.org/10.1016/s0378-4347(99)00045-6
TI - Analysis of diagnostic metabolites by capillary electrophoresis–mass spectrometry
T2 - Journal of Chromatography B Biomedical Sciences and Applications
AU - Chen, Yong
AU - Quinn, Douglas
AU - Fu, Emil
AU - Wang, Y.Karen
PY - 1999
DA - 1999/04/01 00:00:00
PB - Elsevier
SP - 43-52
IS - 1-2
VL - 727
SN - 0378-4347
ER -
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@article{1999_Chen,
author = {Yong Chen and Douglas Quinn and Emil Fu and Y.Karen Wang},
title = {Analysis of diagnostic metabolites by capillary electrophoresis–mass spectrometry},
journal = {Journal of Chromatography B Biomedical Sciences and Applications},
year = {1999},
volume = {727},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016/s0378-4347(99)00045-6},
number = {1-2},
pages = {43--52},
doi = {10.1016/s0378-4347(99)00045-6}
}
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MLA
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Chen, Yong, et al. “Analysis of diagnostic metabolites by capillary electrophoresis–mass spectrometry.” Journal of Chromatography B Biomedical Sciences and Applications, vol. 727, no. 1-2, Apr. 1999, pp. 43-52. https://doi.org/10.1016/s0378-4347(99)00045-6.