Toxicology in Vitro

, volume 2 , issue 1 , pages 1-6

Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests.

E. Borenfreund ¹

H. Babich ¹

N Martín Alguacil ¹

Hide authors affiliations Show authors affiliations: 1 affiliation

Laboratory Animal Research Center, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA |

Publication type: Journal Article

Publication date: 1988-01-01

Elsevier

Toxicology in Vitro

scimago Q2

wos Q3

SJR: 0.718

CiteScore: 5.5

Impact factor: 2.7

ISSN: 08872333, 18793177

DOI: 10.1016/0887-2333(88)90030-6

Copy DOI

PubMed ID: 20702351

General Medicine

Toxicology

Abstract

The neutral red (NR) and tetrazolium MTT in vitro cytotoxicity assays were compared for 28 test agents of widely varying potency using the BALB/c mouse 3T3 fibroblast cell line as the bioindicator. For any given cell density in the microtitre plate well, the optical density absorbance with the NR assay was about twice that obtained with the MTT assay. However, there was good agreement (r = 0.939) between the ranking of the test agents on the basis of midpoint cytotoxicity values (NR(50) and MTT(50)), although the assays were based on different physiological endpoints. Nevertheless, the two assays were found to differ in sensitivity for a few test agents, such as chloroquine sulphate and several antineoplastic drugs. Both assays were capable of demonstrating a reduction in the cytotoxicity of methotrexate by leucovorin.

Found

2 citations

Vieira Rosemary

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Universidade Federal Fluminense

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Simonova Yulia

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A.V. Topchiev Institute of Petrochemical Synthesis RAS

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Ivanov Aleksandr

DSc in Biological/biomedical sciences

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Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences

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HIV

Reactive oxygen species

1 citation

Isakova-Sivak Irina

DSc in Health sciences, associate member of the Russian Academy of Sciences

112 publications, 1 537 citations, 31 reviews

h-index: 22

Institute of Experimental Medicine

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Cite this

GOST |

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GOST Copy

Borenfreund E., Babich H., Martín Alguacil N. Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests. // Toxicology in Vitro. 1988. Vol. 2. No. 1. pp. 1-6.

GOST all authors (up to 50) Copy

Borenfreund E., Babich H., Martín Alguacil N. Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests. // Toxicology in Vitro. 1988. Vol. 2. No. 1. pp. 1-6.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1016/0887-2333(88)90030-6

UR - https://doi.org/10.1016/0887-2333(88)90030-6

TI - Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests.

T2 - Toxicology in Vitro

AU - Borenfreund, E.

AU - Babich, H.

AU - Martín Alguacil, N

PY - 1988

DA - 1988/01/01

PB - Elsevier

SP - 1-6

IS - 1

VL - 2

PMID - 20702351

SN - 0887-2333

SN - 1879-3177

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{1988_Borenfreund,

author = {E. Borenfreund and H. Babich and N Martín Alguacil},

title = {Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests.},

journal = {Toxicology in Vitro},

year = {1988},

volume = {2},

publisher = {Elsevier},

month = {jan},

url = {https://doi.org/10.1016/0887-2333(88)90030-6},

number = {1},

pages = {1--6},

doi = {10.1016/0887-2333(88)90030-6}

}

MLA

Cite this

MLA Copy

Borenfreund, E., et al. “Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests..” Toxicology in Vitro, vol. 2, no. 1, Jan. 1988, pp. 1-6. https://doi.org/10.1016/0887-2333(88)90030-6.

Publisher

Elsevier

Journal

Toxicology in Vitro

scimago Q2

wos Q3

SJR

0.718

CiteScore

5.5

Impact factor

2.7

ISSN

08872333 (Print)

18793177 (Electronic)